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mmu_circUsp32_1185
Basic information of circular RNA
PanCircBase ID:
mmu_circUsp32_1185
mm39_Chr. Position ID:
chr11|84917601|84933506|-
Gene name:
Usp32
Transcript:
ENSMUST00000108075.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
21,20,19,18,17,16,15,14,13
Exon/intron offsets:
0,325,2285,3707,5293,9746,12425,13219,15712
Genomic length:
15905
Exon/intron sizes in nt:
10,61,39,75,82,15,51,10,00,00,00,000
Flanking exon/introns:
chr11:84916518-84917601|chr11:84933506-84935535
Splice length:
1185
Other database IDs:
CIRCpedia: HSA_CIRCpedia_375672
Human Ortholog IDs:
Mature spliced Sequence:
GATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGCGATTTGGTGCAAGAAC
59.69
AGGATGTGCTGCTGTTCCAA
59.89
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGCGATTTGGTGCAAGAACT
59.4
AGGATGTGCTGCTGTTCCAA
59.89
141
PCR template sequence:
TCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGGGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
17
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-106a-5p(1), mmu-miR-93-5p(1), mmu-miR-20a-5p(1), mmu-miR-17-5p(1), mmu-miR-135a-5p(1), mmu-miR-106b-5p(1), mmu-miR-196a-5p(1), mmu-miR-143-3p(1), mmu-miR-24-3p(1), mmu-miR-136-5p(1), mmu-miR-1198-5p(1), mmu-miR-344d-3p(1), mmu-miR-207(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTTAAAGCTCCGGGATGCCAGCCCTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUSP32_1185_ORF_1
0.8649
0.085598968
0.999997493
2250
ORF sequence:
ATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGGGATGCCAGCCCTGTTGTAATTGAGCCATCATCTGTTCTAAATGGAGGAAAATTTTCATTTGGAACAGCAGCACATCCTATAGAGCATGGAGAAGATAGAATCAGTAATAACCTTGGTTATATGAATACCACAGAAGAGAAATACTCAGACAACATTTCTTCTGCATCTGAAGCCTCAGAGTCTACTGGCAGTGGCTTTCTGTATTCTGGCACACCAGGGGCAGATATGTGCTTTGCAAGGCAGCATAACACTTCTGACAATAACAACCAGTGTTTACTAGGAGCCAATGGGAATATCCTTTTGCACCTTAATCCTCAGAAGCCAGGAGCAATTGATAACCAGCCACTAGTAACTCAGGAACCAGTAAAGGCTACATCATTAACACTAGAAGGAGGGCGATTAAAACGAACTCCACAGCTAATCCATGGAAGAGATTATGAAATGGTCCCAGAACCCGTGTGGAGAGCACTTTATCATTGGTATGGATCAAACCTGGCCCTTCCTAGACCAGTAATCAAGAATAGCAAAACGGACATCCCAGAACTAGAATTATTTCCTCGCTATCTTCTCTTCCTTCGACAGCAGCCTGCCACACGGACACAGCAATCTAACATCTGGGTGAATATGGGAAATGTACCCTCTCCAAATGCGCCTCTAAAGCGAGTGTTAGCCTATACAGGCTGTTTTAGTAGAATGCAAACCATCAAGGAAATTCATGAATATCTGTCTCAAAGGCTTCGCATTAAAGAGGAAGATATGCGGCTGTGGCTGTACAATAGTGAGAACTACCTGACTCTTCTGGATGATGAGGATCACAGATTGGAATATTTGAAAATCCAGGATGAGCAGCACCTGGTAATTGAAGTTCGAAACAAAGATATGAGTTGGCCTGAAGAAATGTCTTTTATAGCAAACAGTAGCAAAATAGATAGACATAAAGTTCCCACAGAAAAGGGCGCCACAGGTCTGAGTAACCTGGGAAACACATGCTTCATGAACTCAAGCATCCAGTGTGTCAGCAATACACAACCACTGACACAGTACTTTATCTCAGGGAGGCATCTTTATGAACTCAACAGGACAAATCCCATTGGTATGAAAGGCCATATGGCTAAATGTTATGGCGATTTGGTGCAAGAACTTTGGAGTGGAACTCAGAAGAATGTTGCCCCCTTAAAGCTCCGG
Peptide sequence:
MNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLRDASPVVIEPSSVLNGGKFSFGTAAHPIEHGEDRISNNLGYMNTTEEKYSDNISSASEASESTGSGFLYSGTPGADMCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAIDNQPLVTQEPVKATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLALPRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGNVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLWLYNSENYLTLLDDEDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANSSKIDRHKVPTEKGATGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKLR
IRESfinder result
IRESfinder score
0.635008663
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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