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mmu_circUros_659
Basic information of circular RNA
PanCircBase ID:
mmu_circUros_659
mm39_Chr. Position ID:
chr7|133292236|133292895|-
Gene name:
Uros
Transcript:
ENSMUST00000135760.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
7
Exon/intron offsets:
0
Genomic length:
659
Exon/intron sizes in nt:
659
Flanking exon/introns:
chr7:133291409-133292236|chr7:133292895-133299397
Splice length:
659
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGCCATCTTCAGAGCTGCCTCTTCTCTTTCCGTGTGGAACTATCAAAGGAGATACTCTTCCAAAAATGCTCAAGGACAAAGGTGACTAGAATTAGCTAAGGACACCTCTAAAGATGGTCCTGAGACCCAGGAGGCAGACGTGACCATGTTCAGGCAGAGTTGGGATGCAGCGGGTCTCCTGATGATGCTAAGCCTTTTTTGAGAGCTGTCCTAGACTGGCTGCCAACATGGCTGTGGGATTTATGGAGCAGCCAAGATCAGAGCTGTGTGGACACTCACACAGACACGGGAACCAGCAGCAAGTGTGAGTATGGAGGAAGGCTGTGCTGTCTGGACCTCTCTTTGCTGACTCAGCCTTCCCACCTGTGGATTGATTCCATGGAGCTCTGCAGGTCACTCAGGGCTGGGCAAGGCCTTTTGCCCCCTAGTTGAGCTTCCCATTTCTGAGGAGCTGGTTCTCAGAGGTATGGTGACCGTCCCCATCAGCTACACACTGCAGAGGGTGCAGAGCCCCCATTCCCACTTCCATTCCCTGTAAAGTGAAGCCTGTCTGATGGTTTTAATTGTACCCAGGGATCCCCATGGAAAGCATGCATGTCTATCAGACAGTTCCACACCCTGGGATCCAAGGGAGCCTGAAGAGCTACTATGAAGATCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCCCCATGGAAAGCATGCAT
60.03
AGTCACCTTTGTCCTTGAGCA
59.51
170
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCCCCATGGAAAGCATGCA
60.03
AGTCACCTTTGTCCTTGAGCA
59.51
171
PCR template sequence:
TTAATTGTACCCAGGGATCCCCATGGAAAGCATGCATGTCTATCAGACAGTTCCACACCCTGGGATCCAAGGGAGCCTGAAGAGCTACTATGAAGATCAGAGCCATCTTCAGAGCTGCCTCTTCTCTTTCCGTGTGGAACTATCAAAGGAGATACTCTTCCAAAAATGCTCAAGGACAAAGGTGACTAGAATTAGCTAAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-351-5p(1), mmu-miR-673-5p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTATGAAGATCAGAGCCATCTTCAGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUROS_659_ORF_1
0.762
0.010182465
0.073239319
177
ORF sequence:
ATGGAAAGCATGCATGTCTATCAGACAGTTCCACACCCTGGGATCCAAGGGAGCCTGAAGAGCTACTATGAAGATCAGAGCCATCTTCAGAGCTGCCTCTTCTCTTTCCGTGTGGAACTATCAAAGGAGATACTCTTCCAAAAATGCTCAAGGACAAAGGTGACTAGAATTAGCTAA
Peptide sequence:
MESMHVYQTVPHPGIQGSLKSYYEDQSHLQSCLFSFRVELSKEILFQKCSRTKVTRIS
IRESfinder result
IRESfinder score
0.616015113
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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