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mmu_circUnkl_482
Basic information of circular RNA
PanCircBase ID:
mmu_circUnkl_482
mm39_Chr. Position ID:
chr17|25424572|25432080|+
Gene name:
Unkl
Transcript:
ENSMUST00000039734.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,2490,3955,5205,7448
Genomic length:
7508
Exon/intron sizes in nt:
136,118,85,83,60
Flanking exon/introns:
chr17:25420149-25424572|chr17:25432080-25437551
Splice length:
482
Other database IDs:
CIRCpedia: MMU_CIRCpedia_217521, circAtlas: mmu-Unkl_0004
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
ACAGTAACTTCGTATTGGGCAGCTATAAGACTGAGCAGTGCCCGAAGCCACCACGCCTATGTCGCCAGGGCTACGCCTGTCCACACTACCACAACAGCAGAGACCGCCGTCGGAACCCCAGGAGGTTCCAGTACAGGTCTACGCCCTGTCCTAGTGTGAAGCACGGTGACGAGTGGGGCGAGCCCTCACGGTGTGACGGTGGGGACAGCTGTCAGTACTGCCATTCCCGCACGGAGCAGCAGTTCCATCCTGAGATCTACAAATCTACAAAATGCAACGATATGCGCCAAACTGGCTACTGCCCTCGGGGCCCTTTCTGTGCCTTTGCACACACTGAGAAGAGCCTGGCTATGGTGAACGAGTGGAGCTGCCGTGATCTCAGTTCCAACAGCACCTCAGCTTATAGCAGCCAGCCTGGAAGTGCCAAAAGGAAAGACTCCCCCTCTGAAGGAAGCCAGAAAGCTACTGAGGACAGCAAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTTATAGCAGCCAGCCTG
59.89
TTGTGGTAGTGTGGACAGGC
59.89
179
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGCTTATAGCAGCCAGCCT
59.89
TTGTGGTAGTGTGGACAGGC
59.89
180
PCR template sequence:
TTCCAACAGCACCTCAGCTTATAGCAGCCAGCCTGGAAGTGCCAAAAGGAAAGACTCCCCCTCTGAAGGAAGCCAGAAAGCTACTGAGGACAGCAAGCAGACAGTAACTTCGTATTGGGCAGCTATAAGACTGAGCAGTGCCCGAAGCCACCACGCCTATGTCGCCAGGGCTACGCCTGTCCACACTACCACAACAGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-19b-3p(1), mmu-miR-19a-3p(1), mmu-miR-22-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGACAGCAAGCAGACAGTAACTTCGT
siRNA sets
TACTGTCTGCTTGCTGTCC, TTACTGTCTGCTTGCTGTC, AGTTACTGTCTGCTTGCTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUNKL_482_ORF_1
1.0414
0.19800432
0.519594375
360
ORF sequence:
ATGCGCCAAACTGGCTACTGCCCTCGGGGCCCTTTCTGTGCCTTTGCACACACTGAGAAGAGCCTGGCTATGGTGAACGAGTGGAGCTGCCGTGATCTCAGTTCCAACAGCACCTCAGCTTATAGCAGCCAGCCTGGAAGTGCCAAAAGGAAAGACTCCCCCTCTGAAGGAAGCCAGAAAGCTACTGAGGACAGCAAGCAGACAGTAACTTCGTATTGGGCAGCTATAAGACTGAGCAGTGCCCGAAGCCACCACGCCTATGTCGCCAGGGCTACGCCTGTCCACACTACCACAACAGCAGAGACCGCCGTCGGAACCCCAGGAGGTTCCAGTACAGGTCTACGCCCTGTCCTAGTGTGA
Peptide sequence:
MRQTGYCPRGPFCAFAHTEKSLAMVNEWSCRDLSSNSTSAYSSQPGSAKRKDSPSEGSQKATEDSKQTVTSYWAAIRLSSARSHHAYVARATPVHTTTTAETAVGTPGGSSTGLRPVLV
IRESfinder result
IRESfinder score
0.321676852
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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