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mmu_circUnc80_799
Basic information of circular RNA
PanCircBase ID:
mmu_circUnc80_799
mm39_Chr. Position ID:
chr1|66588886|66609161|+
Gene name:
Unc80
Transcript:
ENSMUST00000061620.17
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
19,20,21,22,23
Exon/intron offsets:
0,1218,2426,5199,20062
Genomic length:
20275
Exon/intron sizes in nt:
2,09,10,79,71,73,213
Flanking exon/introns:
chr1:66570012-66588886|chr1:66609161-66629353
Splice length:
799
Other database IDs:
CIRCpedia: MMU_CIRCpedia_26591, circAtlas: mmu-Unc80_0016
Human Ortholog IDs:
Mature spliced Sequence:
TGATGAACCAATGCAAGGGGGCAACCTGGGACGGAAAGATTTCTGGCGCAAGATGTTCAAATCCCAAAGCGCAGCAAGTGACACCAGCAGCCAGTCTGAGCAGGATACCTCGGAATGTACCACAGCCCACTCAGGGAACACCTCTGACCGGCGTGCCCGCTCACGATCCCGCAGAATCTCCCTGCGAAAGAAGTTGAAACTCCCCATAGGAAACTGGCTGAAGCGCTCCTCCCTCTCTGGCCTGGCAGATGGTGTAGAGGACCTCCTGGACATTAGCTCTGTGGATCGCCTGTCTTTCATCAGGCAAAGCTCCAAGGTCAAGTTCACCAGCGCTGTCAAGCTCTCAGAAGGTGGACCAGGGAGTGGGATGGAGAATGGACGAGAAGAGGAGGAGAATTTCTTCAAGCGTCTTGGCTGCCACAGTTTCGATGACCACCTTTCACCCAACCAAGATGGTGGGAAAAGCAAAAACGTGGTGAATCTGGGAGCAATCCGACAAGGCATGAAACGTTTTCAATTCCTGTTGAACTGCTGTGAGCCAGGGACAATTCCTGATGCATCCATCCTAGCTGCTGCCTTGGACTTAGAAGCTCCTGTGGTGGCCAGAGCAGCTCTGTTCCTCGAATGTGCCCGTTTTGTCCACCGGTGCAACCGTGGCAATTGGCCCGAGTGGATGAAAGGGCACCACGTGAACATCACCAAGAAAGGCCTTTCCCGGGGCAGGTCTCCCACCGTGGGCAACAAGCGGAACCAGAAGCTTCAGTGGAGTGCTGCCAAGCTCTTCTACCAGTGGGGAGAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAACAAGCGGAACCAGAAG
60.04
CTTGCTGCGCTTTGGGATTT
60.04
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAACAAGCGGAACCAGAAGC
60.04
GTGTCACTTGCTGCGCTTTG
60.93
145
PCR template sequence:
CAAGAAAGGCCTTTCCCGGGGCAGGTCTCCCACCGTGGGCAACAAGCGGAACCAGAAGCTTCAGTGGAGTGCTGCCAAGCTCTTCTACCAGTGGGGAGATTGATGAACCAATGCAAGGGGGCAACCTGGGACGGAAAGATTTCTGGCGCAAGATGTTCAAATCCCAAAGCGCAGCAAGTGACACCAGCAGCCAGTCTGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-33-5p(1), mmu-miR-666-5p(1), mmu-miR-540-3p(1), mmu-miR-670-5p(1), mmu-miR-3061-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CCAGTGGGGAGATTGATGAACCAATG
siRNA sets
ATTGGTTCATCAATCTCCC, TTGGTTCATCAATCTCCCC, CATTGGTTCATCAATCTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUNC80_799_ORF_1
0.6992
0.338426557
0.957660637
792
ORF sequence:
ATGCAAGGGGGCAACCTGGGACGGAAAGATTTCTGGCGCAAGATGTTCAAATCCCAAAGCGCAGCAAGTGACACCAGCAGCCAGTCTGAGCAGGATACCTCGGAATGTACCACAGCCCACTCAGGGAACACCTCTGACCGGCGTGCCCGCTCACGATCCCGCAGAATCTCCCTGCGAAAGAAGTTGAAACTCCCCATAGGAAACTGGCTGAAGCGCTCCTCCCTCTCTGGCCTGGCAGATGGTGTAGAGGACCTCCTGGACATTAGCTCTGTGGATCGCCTGTCTTTCATCAGGCAAAGCTCCAAGGTCAAGTTCACCAGCGCTGTCAAGCTCTCAGAAGGTGGACCAGGGAGTGGGATGGAGAATGGACGAGAAGAGGAGGAGAATTTCTTCAAGCGTCTTGGCTGCCACAGTTTCGATGACCACCTTTCACCCAACCAAGATGGTGGGAAAAGCAAAAACGTGGTGAATCTGGGAGCAATCCGACAAGGCATGAAACGTTTTCAATTCCTGTTGAACTGCTGTGAGCCAGGGACAATTCCTGATGCATCCATCCTAGCTGCTGCCTTGGACTTAGAAGCTCCTGTGGTGGCCAGAGCAGCTCTGTTCCTCGAATGTGCCCGTTTTGTCCACCGGTGCAACCGTGGCAATTGGCCCGAGTGGATGAAAGGGCACCACGTGAACATCACCAAGAAAGGCCTTTCCCGGGGCAGGTCTCCCACCGTGGGCAACAAGCGGAACCAGAAGCTTCAGTGGAGTGCTGCCAAGCTCTTCTACCAGTGGGGAGATTGA
Peptide sequence:
MQGGNLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGNTSDRRARSRSRRISLRKKLKLPIGNWLKRSSLSGLADGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGPGSGMENGREEEENFFKRLGCHSFDDHLSPNQDGGKSKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARAALFLECARFVHRCNRGNWPEWMKGHHVNITKKGLSRGRSPTVGNKRNQKLQWSAAKLFYQWGD
IRESfinder result
IRESfinder score
0.388459186
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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