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mmu_circUnc80_605
Basic information of circular RNA
PanCircBase ID:
mmu_circUnc80_605
mm39_Chr. Position ID:
chr1|66632823|66647651|+
Gene name:
Unc80
Transcript:
ENSMUST00000061620.17
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
25,26,27,28
Exon/intron offsets:
0,7390,12787,14727
Genomic length:
14828
Exon/intron sizes in nt:
1,34,16,62,04,101
Flanking exon/introns:
chr1:66629489-66632823|chr1:66647651-66651246
Splice length:
605
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTACTGTGAACCCCTCTAAATGTGGCTGTCCCTTTGCCTTGAAGATGGCAGCATGCCAACTTCTCCTGGAGATAACCACCTTCCTGAGGGAGACTTTTTCATGCCTGCCTAGACCTCGCACTGAGCCCCTCGTGGACTTGGAAAGCTGCAGGCTTCGTCTGGACCCTGAATTGGACCGGCACAGATATGAGAGGAAGATCAGCTTTGCAGGAGTTCTGGATGAAAATGAAGACTCAAAAGATTCTCTGCACAGCAGCAGCCACACCATCAAATCAGACGCAGGTGCTGAAGAGAAGAAAGTTCCCAGCAGGAAGATCAGGATAGGAGGTTCTCGACTGCTCCAGATTAAAGGAACCCGCAGTTTCCAGGTGAAGAAGGGGGGTTCCTTGTCCAGCATTCGCCGAGTCGGCAGCTTAAAGAGCAGCAAGTTATCACGGCAGGACTCAGAGTCTGAGGCTGAGGAGCTGCAGCTGTCCCAGAGCAGGGACACTGTCACTGACCTAGAAGGAAGTCCTTGGAGTGCAAGTGAACCCAGCATTGAGCCTGAGGGACTGAGCAATGCCGGCACAGAGGAAAACTACCACAGAAACATGTCGTGGCTTCAC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGTGCAAGTGAACCCAGCAT
60.18
GCATGCTGCCATCTTCAAGG
59.9
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGTGCAAGTGAACCCAG
60.18
GCATGCTGCCATCTTCAAGG
59.9
146
PCR template sequence:
AGGAAGTCCTTGGAGTGCAAGTGAACCCAGCATTGAGCCTGAGGGACTGAGCAATGCCGGCACAGAGGAAAACTACCACAGAAACATGTCGTGGCTTCACGTACTGTGAACCCCTCTAAATGTGGCTGTCCCTTTGCCTTGAAGATGGCAGCATGCCAACTTCTCCTGGAGATAACCACCTTCCTGAGGGAGACTTTTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-22-3p(1), mmu-miR-370-3p(1), mmu-miR-335-3p(1), mmu-miR-484(1), mmu-miR-1199-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTCGTGGCTTCACGTACTGTGAACCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUNC80_605_ORF_1
0.7895
0.253348267
0.79345964
570
ORF sequence:
ATGGCAGCATGCCAACTTCTCCTGGAGATAACCACCTTCCTGAGGGAGACTTTTTCATGCCTGCCTAGACCTCGCACTGAGCCCCTCGTGGACTTGGAAAGCTGCAGGCTTCGTCTGGACCCTGAATTGGACCGGCACAGATATGAGAGGAAGATCAGCTTTGCAGGAGTTCTGGATGAAAATGAAGACTCAAAAGATTCTCTGCACAGCAGCAGCCACACCATCAAATCAGACGCAGGTGCTGAAGAGAAGAAAGTTCCCAGCAGGAAGATCAGGATAGGAGGTTCTCGACTGCTCCAGATTAAAGGAACCCGCAGTTTCCAGGTGAAGAAGGGGGGTTCCTTGTCCAGCATTCGCCGAGTCGGCAGCTTAAAGAGCAGCAAGTTATCACGGCAGGACTCAGAGTCTGAGGCTGAGGAGCTGCAGCTGTCCCAGAGCAGGGACACTGTCACTGACCTAGAAGGAAGTCCTTGGAGTGCAAGTGAACCCAGCATTGAGCCTGAGGGACTGAGCAATGCCGGCACAGAGGAAAACTACCACAGAAACATGTCGTGGCTTCACGTACTGTGA
Peptide sequence:
MAACQLLLEITTFLRETFSCLPRPRTEPLVDLESCRLRLDPELDRHRYERKISFAGVLDENEDSKDSLHSSSHTIKSDAGAEEKKVPSRKIRIGGSRLLQIKGTRSFQVKKGGSLSSIRRVGSLKSSKLSRQDSESEAEELQLSQSRDTVTDLEGSPWSASEPSIEPEGLSNAGTEENYHRNMSWLHVL
IRESfinder result
IRESfinder score
0.429368288
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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