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mmu_circUnc80_266
Basic information of circular RNA
PanCircBase ID:
mmu_circUnc80_266
mm39_Chr. Position ID:
chr1|66569130|66570012|+
Gene name:
Unc80
Transcript:
ENSMUST00000061620.17
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
17,18
Exon/intron offsets:
0,783
Genomic length:
882
Exon/intron sizes in nt:
167,99
Flanking exon/introns:
chr1:66567179-66569130|chr1:66570012-66588886
Splice length:
266
Other database IDs:
circAtlas: mmu-Unc80_0031
Human Ortholog IDs:
Mature spliced Sequence:
GGACTGTACTGTGACATCCGCCAGCTGGTTCAGTTTATCAAAGAAGCTCATGGGAATGTCTTCAGGAGAGTGGCTCTTAGTGCCCTGCTTGATAGTGCTGAAAAGTTAGCACCAGGGAAAAAGGTGGAAGAGAATGGACAGGAGTCTAAGCCTGTGGGCAGTAAAAGGTCAGAAGCAGGAAGCATTGCAGATAAAGGCCAGGTATCGTCAGCACCTGAGGAATGTCGAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAACA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGTCAGCACCTGAGGAATGT
60.04
TCAAGCAGGGCACTAAGAGC
60.04
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTATCGTCAGCACCTGAGG
59.9
TCAAGCAGGGCACTAAGAGC
60.04
158
PCR template sequence:
GGTCAGAAGCAGGAAGCATTGCAGATAAAGGCCAGGTATCGTCAGCACCTGAGGAATGTCGAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAACAGGACTGTACTGTGACATCCGCCAGCTGGTTCAGTTTATCAAAGAAGCTCATGGGAATGTCTTCAGGAGAGTGGCTCTTAGTGCCCTGCTTGATAGTGCTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
AGACCCCAGAACAGGACTGTACTGTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUNC80_266_ORF_1
0.7202
0.237925365
0.328792731
309
ORF sequence:
ATGTCGAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAACAGGACTGTACTGTGACATCCGCCAGCTGGTTCAGTTTATCAAAGAAGCTCATGGGAATGTCTTCAGGAGAGTGGCTCTTAGTGCCCTGCTTGATAGTGCTGAAAAGTTAGCACCAGGGAAAAAGGTGGAAGAGAATGGACAGGAGTCTAAGCCTGTGGGCAGTAAAAGGTCAGAAGCAGGAAGCATTGCAGATAAAGGCCAGGTATCGTCAGCACCTGAGGAATGTCGAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAA
Peptide sequence:
MSKLHVWSPLTDPRTGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKLAPGKKVEENGQESKPVGSKRSEAGSIADKGQVSSAPEECRSFMSGRPSQTPE
IRESfinder result
IRESfinder score
0.454498423
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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