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mmu_circUnc80_1350
Basic information of circular RNA
PanCircBase ID:
mmu_circUnc80_1350
mm39_Chr. Position ID:
chr1|66567030|66629489|+
Gene name:
Unc80
Transcript:
ENSMUST00000061620.17
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
16,17,18,19,20,21,22,23,24
Exon/intron offsets:
0,2100,2883,21856,23074,24282,27055,41918,62323
Genomic length:
62459
Exon/intron sizes in nt:
149,167,99,209,107,97,173,213,136
Flanking exon/introns:
chr1:66564949-66567030|chr1:66629489-66632823
Splice length:
1350
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACAAAGCTGGGTTTGGAAATAACTTCACCACAGTGGACAACAAGTCCACAGCCCAAAATGTGGAAGGCATTATTGTCGGTGCCATGTTCAAGTCTCTCATCACACGCTGTGCCTCAACCACACATGAACTCCACAGCCCTGAGAATCTGGGACTGTACTGTGACATCCGCCAGCTGGTTCAGTTTATCAAAGAAGCTCATGGGAATGTCTTCAGGAGAGTGGCTCTTAGTGCCCTGCTTGATAGTGCTGAAAAGTTAGCACCAGGGAAAAAGGTGGAAGAGAATGGACAGGAGTCTAAGCCTGTGGGCAGTAAAAGGTCAGAAGCAGGAAGCATTGCAGATAAAGGCCAGGTATCGTCAGCACCTGAGGAATGTCGAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAACATGATGAACCAATGCAAGGGGGCAACCTGGGACGGAAAGATTTCTGGCGCAAGATGTTCAAATCCCAAAGCGCAGCAAGTGACACCAGCAGCCAGTCTGAGCAGGATACCTCGGAATGTACCACAGCCCACTCAGGGAACACCTCTGACCGGCGTGCCCGCTCACGATCCCGCAGAATCTCCCTGCGAAAGAAGTTGAAACTCCCCATAGGAAACTGGCTGAAGCGCTCCTCCCTCTCTGGCCTGGCAGATGGTGTAGAGGACCTCCTGGACATTAGCTCTGTGGATCGCCTGTCTTTCATCAGGCAAAGCTCCAAGGTCAAGTTCACCAGCGCTGTCAAGCTCTCAGAAGGTGGACCAGGGAGTGGGATGGAGAATGGACGAGAAGAGGAGGAGAATTTCTTCAAGCGTCTTGGCTGCCACAGTTTCGATGACCACCTTTCACCCAACCAAGATGGTGGGAAAAGCAAAAACGTGGTGAATCTGGGAGCAATCCGACAAGGCATGAAACGTTTTCAATTCCTGTTGAACTGCTGTGAGCCAGGGACAATTCCTGATGCATCCATCCTAGCTGCTGCCTTGGACTTAGAAGCTCCTGTGGTGGCCAGAGCAGCTCTGTTCCTCGAATGTGCCCGTTTTGTCCACCGGTGCAACCGTGGCAATTGGCCCGAGTGGATGAAAGGGCACCACGTGAACATCACCAAGAAAGGCCTTTCCCGGGGCAGGTCTCCCACCGTGGGCAACAAGCGGAACCAGAAGCTTCAGTGGAGTGCTGCCAAGCTCTTCTACCAGTGGGGAGATGCAATTGGCATCCGATTGAATGAGCTGTGCCATGGAGAGAGCGAGAGTCCTGCCAACCTGCTCGGCCTTATCTATGATGAGGAAACCAAGAGAAGACTGCGGAAGGAGGATGAGGAGGAAGACTTTTTAGATGACA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAACCTGCTCGGCCTTATCT
59.82
GCCTTCCACATTTTGGGCTG
60.04
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAACCTGCTCGGCCTTATCT
59.82
ACTTGAACATGGCACCGACA
60.18
176
PCR template sequence:
GAGAGCGAGAGTCCTGCCAACCTGCTCGGCCTTATCTATGATGAGGAAACCAAGAGAAGACTGCGGAAGGAGGATGAGGAGGAAGACTTTTTAGATGACAACAAAGCTGGGTTTGGAAATAACTTCACCACAGTGGACAACAAGTCCACAGCCCAAAATGTGGAAGGCATTATTGTCGGTGCCATGTTCAAGTCTCTCAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-183-5p(2), mmu-miR-27a-3p(1), mmu-miR-33-5p(1), mmu-miR-666-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-540-3p(1), mmu-miR-670-5p(1), mmu-miR-3061-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTTTTAGATGACAACAAAGCTGGGTT
siRNA sets
AACCCAGCTTTGTTGTCAT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUNC80_1350_ORF_1
0.8631
0.350647653
0.999999939
2616
ORF sequence:
ATGTTCAAGTCTCTCATCACACGCTGTGCCTCAACCACACATGAACTCCACAGCCCTGAGAATCTGGGACTGTACTGTGACATCCGCCAGCTGGTTCAGTTTATCAAAGAAGCTCATGGGAATGTCTTCAGGAGAGTGGCTCTTAGTGCCCTGCTTGATAGTGCTGAAAAGTTAGCACCAGGGAAAAAGGTGGAAGAGAATGGACAGGAGTCTAAGCCTGTGGGCAGTAAAAGGTCAGAAGCAGGAAGCATTGCAGATAAAGGCCAGGTATCGTCAGCACCTGAGGAATGTCGAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAACATGATGAACCAATGCAAGGGGGCAACCTGGGACGGAAAGATTTCTGGCGCAAGATGTTCAAATCCCAAAGCGCAGCAAGTGACACCAGCAGCCAGTCTGAGCAGGATACCTCGGAATGTACCACAGCCCACTCAGGGAACACCTCTGACCGGCGTGCCCGCTCACGATCCCGCAGAATCTCCCTGCGAAAGAAGTTGAAACTCCCCATAGGAAACTGGCTGAAGCGCTCCTCCCTCTCTGGCCTGGCAGATGGTGTAGAGGACCTCCTGGACATTAGCTCTGTGGATCGCCTGTCTTTCATCAGGCAAAGCTCCAAGGTCAAGTTCACCAGCGCTGTCAAGCTCTCAGAAGGTGGACCAGGGAGTGGGATGGAGAATGGACGAGAAGAGGAGGAGAATTTCTTCAAGCGTCTTGGCTGCCACAGTTTCGATGACCACCTTTCACCCAACCAAGATGGTGGGAAAAGCAAAAACGTGGTGAATCTGGGAGCAATCCGACAAGGCATGAAACGTTTTCAATTCCTGTTGAACTGCTGTGAGCCAGGGACAATTCCTGATGCATCCATCCTAGCTGCTGCCTTGGACTTAGAAGCTCCTGTGGTGGCCAGAGCAGCTCTGTTCCTCGAATGTGCCCGTTTTGTCCACCGGTGCAACCGTGGCAATTGGCCCGAGTGGATGAAAGGGCACCACGTGAACATCACCAAGAAAGGCCTTTCCCGGGGCAGGTCTCCCACCGTGGGCAACAAGCGGAACCAGAAGCTTCAGTGGAGTGCTGCCAAGCTCTTCTACCAGTGGGGAGATGCAATTGGCATCCGATTGAATGAGCTGTGCCATGGAGAGAGCGAGAGTCCTGCCAACCTGCTCGGCCTTATCTATGATGAGGAAACCAAGAGAAGACTGCGGAAGGAGGATGAGGAGGAAGACTTTTTAGATGACAACAAAGCTGGGTTTGGAAATAACTTCACCACAGTGGACAACAAGTCCACAGCCCAAAATGTGGAAGGCATTATTGTCGGTGCCATGTTCAAGTCTCTCATCACACGCTGTGCCTCAACCACACATGAACTCCACAGCCCTGAGAATCTGGGACTGTACTGTGACATCCGCCAGCTGGTTCAGTTTATCAAAGAAGCTCATGGGAATGTCTTCAGGAGAGTGGCTCTTAGTGCCCTGCTTGATAGTGCTGAAAAGTTAGCACCAGGGAAAAAGGTGGAAGAGAATGGACAGGAGTCTAAGCCTGTGGGCAGTAAAAGGTCAGAAGCAGGAAGCATTGCAGATAAAGGCCAGGTATCGTCAGCACCTGAGGAATGTCGAAGCTTCATGTCTGGTCGCCCCTCACAGACCCCAGAACATGATGAACCAATGCAAGGGGGCAACCTGGGACGGAAAGATTTCTGGCGCAAGATGTTCAAATCCCAAAGCGCAGCAAGTGACACCAGCAGCCAGTCTGAGCAGGATACCTCGGAATGTACCACAGCCCACTCAGGGAACACCTCTGACCGGCGTGCCCGCTCACGATCCCGCAGAATCTCCCTGCGAAAGAAGTTGAAACTCCCCATAGGAAACTGGCTGAAGCGCTCCTCCCTCTCTGGCCTGGCAGATGGTGTAGAGGACCTCCTGGACATTAGCTCTGTGGATCGCCTGTCTTTCATCAGGCAAAGCTCCAAGGTCAAGTTCACCAGCGCTGTCAAGCTCTCAGAAGGTGGACCAGGGAGTGGGATGGAGAATGGACGAGAAGAGGAGGAGAATTTCTTCAAGCGTCTTGGCTGCCACAGTTTCGATGACCACCTTTCACCCAACCAAGATGGTGGGAAAAGCAAAAACGTGGTGAATCTGGGAGCAATCCGACAAGGCATGAAACGTTTTCAATTCCTGTTGAACTGCTGTGAGCCAGGGACAATTCCTGATGCATCCATCCTAGCTGCTGCCTTGGACTTAGAAGCTCCTGTGGTGGCCAGAGCAGCTCTGTTCCTCGAATGTGCCCGTTTTGTCCACCGGTGCAACCGTGGCAATTGGCCCGAGTGGATGAAAGGGCACCACGTGAACATCACCAAGAAAGGCCTTTCCCGGGGCAGGTCTCCCACCGTGGGCAACAAGCGGAACCAGAAGCTTCAGTGGAGTGCTGCCAAGCTCTTCTACCAGTGGGGAGATGCAATTGGCATCCGATTGAATGAGCTGTGCCATGGAGAGAGCGAGAGTCCTGCCAACCTGCTCGGCCTTATCTATGATGAGGAAACCAAGAGAAGACTGCGGAAGGAGGATGAGGAGGAAGACTTTTTAGATGAC
Peptide sequence:
MFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKLAPGKKVEENGQESKPVGSKRSEAGSIADKGQVSSAPEECRSFMSGRPSQTPEHDEPMQGGNLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGNTSDRRARSRSRRISLRKKLKLPIGNWLKRSSLSGLADGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGPGSGMENGREEEENFFKRLGCHSFDDHLSPNQDGGKSKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARAALFLECARFVHRCNRGNWPEWMKGHHVNITKKGLSRGRSPTVGNKRNQKLQWSAAKLFYQWGDAIGIRLNELCHGESESPANLLGLIYDEETKRRLRKEDEEEDFLDDNKAGFGNNFTTVDNKSTAQNVEGIIVGAMFKSLITRCASTTHELHSPENLGLYCDIRQLVQFIKEAHGNVFRRVALSALLDSAEKLAPGKKVEENGQESKPVGSKRSEAGSIADKGQVSSAPEECRSFMSGRPSQTPEHDEPMQGGNLGRKDFWRKMFKSQSAASDTSSQSEQDTSECTTAHSGNTSDRRARSRSRRISLRKKLKLPIGNWLKRSSLSGLADGVEDLLDISSVDRLSFIRQSSKVKFTSAVKLSEGGPGSGMENGREEEENFFKRLGCHSFDDHLSPNQDGGKSKNVVNLGAIRQGMKRFQFLLNCCEPGTIPDASILAAALDLEAPVVARAALFLECARFVHRCNRGNWPEWMKGHHVNITKKGLSRGRSPTVGNKRNQKLQWSAAKLFYQWGDAIGIRLNELCHGESESPANLLGLIYDEETKRRLRKEDEEEDFLDD
IRESfinder result
IRESfinder score
0.404793793
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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