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mmu_circUnc80_1313
Basic information of circular RNA
PanCircBase ID:
mmu_circUnc80_1313
mm39_Chr. Position ID:
chr1|66708254|66718902|+
Gene name:
Unc80
Transcript:
ENSMUST00000061620.17
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
52,53,54,55,56,57,58,59
Exon/intron offsets:
0,2490,3561,4891,5791,8220,8969,10468
Genomic length:
10648
Exon/intron sizes in nt:
2,01,12,41,26,12,42,07,00,00,00,000
Flanking exon/introns:
chr1:66693761-66708254|chr1:66718902-66722307
Splice length:
1313
Other database IDs:
circAtlas: mmu-Unc80_0053
Human Ortholog IDs:
Mature spliced Sequence:
GCTGGCAGAAATTGCACACTCCCTCCTGAAGCTGGCACCATATGACACCCAGACAATGGAGAGCCGAGGCCTTCGGCGCTACATCATGGAGATGCTCCCCATTACTGATTGGTCAGCAGAGGCTGTGAGGCCAGCTCTTATCCTCATTCTAAAGAGACTGGATAGAATGTTCAACAAAATCCATAAGATGCCCACCTTGAGGCGACAGGTTGAATGGGAGCCTGCCAGCAGTTTGATTGAAGGGGTTTGTTTGACACTTCAGAGGCAGCCAATCATATCCTTCCTGCCTCACCTTAGGTCACTGATCAATGTCTGTGTCAATCTGGTGATGGGAGTGGTAGGACCTTCCAGTGTTGCGGATGGATTACCCCTTCTTCATCTCAGCCCTTATCTCTCACCACCTCTGCCCTTCAGCACAGCTGTTGTCCGGCTTGTAGCATTGCAGATACAGGCTTTAAAAGAAGATTTTCCTTTAAGCCATGTGATCTCCCCATTCACCAATCAAGAGCGAAGGGAGGGGATGCTTTTAAATCTGCTCATCCCATTTGTGCTCACAGTAGGATCTGGAAGCAAAGATAGCCCGTGGCTGGAGCAGCCCGAGGTACAGCTACTGCTGCAGACTGTCATTAATGTGCTCCTGCCCCCTCGGATCATCAGCACATCCAGGAGCAAGAACTTCATGCTGGAGAGCTCCCCAGCACACTGCTCTACCCCCGGGGATGCAGGCAAAGACTTGCGCAAGGAAGGGCTGGCTGAGTCCACCAGCCAGGCAGCATATTTAGCTCTCAAGGTTATTCTCGTCTGCTTCGAGAGGCAGCTGGGCAGCCAGTGGTACTGGCTGAGCCTGCAGGTGAAGGAGATGGCTCTGCGAAAGGTGGGCGGCCTGGCCCTGTGGGACTTCCTCGACTTCATCGTGCGCACCCGGATACCCATCTTCGTTCTCTTGCGCCCTTTCATCCAATGCAAGCTATTGGCCCAACCAGCAGAAAATCACGAGGAGCTTTCTGCCCGGCAACACATCTCCGACCAGCTGGAGCGGCGCTTCATCCCCCGCCCTTTATGCAAGAGCTCTCTCATTGCAGAGTTCAATAGCGAGCTGAAAATTCTGAAAGAGGCAGTCCACAGTGGATCGGCCTACCAAGGAAAGACATCTATCAGCACTGTAGGCACATCCACTTCTGCCTACCGCCTGAGCCTGGCCACCATGTCTCGCTCCAACACAGGCACAGGCACTGTCTGGGAGCAGGACAGCGAACCATCCCAACAGGCTTCCCAAGACACCCTCAGTCGGACCGATGAGGAGGACGAGGAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATCCCAACAGGCTTCCCAAG
59.96
ATCTCCATGATGTAGCGCCG
60.04
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACAGGCTTCCCAAGACACC
60.18
ATCTCCATGATGTAGCGCCG
60.04
144
PCR template sequence:
TCCAACACAGGCACAGGCACTGTCTGGGAGCAGGACAGCGAACCATCCCAACAGGCTTCCCAAGACACCCTCAGTCGGACCGATGAGGAGGACGAGGAAAGCTGGCAGAAATTGCACACTCCCTCCTGAAGCTGGCACCATATGACACCCAGACAATGGAGAGCCGAGGCCTTCGGCGCTACATCATGGAGATGCTCCCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
14
Interacting miRNAs (#binding sites)
mmu-miR-324-5p(2), mmu-miR-484(2), mmu-miR-181a-5p(1), mmu-miR-122-5p(1), mmu-miR-143-3p(1), mmu-miR-138-5p(1), mmu-miR-139-5p(1), mmu-miR-136-5p(1), mmu-miR-22-3p(1), mmu-miR-215-5p(1), mmu-miR-1198-5p(1), mmu-miR-673-5p(1), mmu-miR-452-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAGGACGAGGAAAGCTGGCAGAAATT
siRNA sets
TTTCTGCCAGCTTTCCTCG, AATTTCTGCCAGCTTTCCT, TTCTGCCAGCTTTCCTCGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUNC80_1313_ORF_1
1.0372
0.262958562
0.999076928
1287
ORF sequence:
ATGGAGAGCCGAGGCCTTCGGCGCTACATCATGGAGATGCTCCCCATTACTGATTGGTCAGCAGAGGCTGTGAGGCCAGCTCTTATCCTCATTCTAAAGAGACTGGATAGAATGTTCAACAAAATCCATAAGATGCCCACCTTGAGGCGACAGGTTGAATGGGAGCCTGCCAGCAGTTTGATTGAAGGGGTTTGTTTGACACTTCAGAGGCAGCCAATCATATCCTTCCTGCCTCACCTTAGGTCACTGATCAATGTCTGTGTCAATCTGGTGATGGGAGTGGTAGGACCTTCCAGTGTTGCGGATGGATTACCCCTTCTTCATCTCAGCCCTTATCTCTCACCACCTCTGCCCTTCAGCACAGCTGTTGTCCGGCTTGTAGCATTGCAGATACAGGCTTTAAAAGAAGATTTTCCTTTAAGCCATGTGATCTCCCCATTCACCAATCAAGAGCGAAGGGAGGGGATGCTTTTAAATCTGCTCATCCCATTTGTGCTCACAGTAGGATCTGGAAGCAAAGATAGCCCGTGGCTGGAGCAGCCCGAGGTACAGCTACTGCTGCAGACTGTCATTAATGTGCTCCTGCCCCCTCGGATCATCAGCACATCCAGGAGCAAGAACTTCATGCTGGAGAGCTCCCCAGCACACTGCTCTACCCCCGGGGATGCAGGCAAAGACTTGCGCAAGGAAGGGCTGGCTGAGTCCACCAGCCAGGCAGCATATTTAGCTCTCAAGGTTATTCTCGTCTGCTTCGAGAGGCAGCTGGGCAGCCAGTGGTACTGGCTGAGCCTGCAGGTGAAGGAGATGGCTCTGCGAAAGGTGGGCGGCCTGGCCCTGTGGGACTTCCTCGACTTCATCGTGCGCACCCGGATACCCATCTTCGTTCTCTTGCGCCCTTTCATCCAATGCAAGCTATTGGCCCAACCAGCAGAAAATCACGAGGAGCTTTCTGCCCGGCAACACATCTCCGACCAGCTGGAGCGGCGCTTCATCCCCCGCCCTTTATGCAAGAGCTCTCTCATTGCAGAGTTCAATAGCGAGCTGAAAATTCTGAAAGAGGCAGTCCACAGTGGATCGGCCTACCAAGGAAAGACATCTATCAGCACTGTAGGCACATCCACTTCTGCCTACCGCCTGAGCCTGGCCACCATGTCTCGCTCCAACACAGGCACAGGCACTGTCTGGGAGCAGGACAGCGAACCATCCCAACAGGCTTCCCAAGACACCCTCAGTCGGACCGATGAGGAGGACGAGGAAAGCTGGCAGAAATTGCACACTCCCTCCTGA
Peptide sequence:
MESRGLRRYIMEMLPITDWSAEAVRPALILILKRLDRMFNKIHKMPTLRRQVEWEPASSLIEGVCLTLQRQPIISFLPHLRSLINVCVNLVMGVVGPSSVADGLPLLHLSPYLSPPLPFSTAVVRLVALQIQALKEDFPLSHVISPFTNQERREGMLLNLLIPFVLTVGSGSKDSPWLEQPEVQLLLQTVINVLLPPRIISTSRSKNFMLESSPAHCSTPGDAGKDLRKEGLAESTSQAAYLALKVILVCFERQLGSQWYWLSLQVKEMALRKVGGLALWDFLDFIVRTRIPIFVLLRPFIQCKLLAQPAENHEELSARQHISDQLERRFIPRPLCKSSLIAEFNSELKILKEAVHSGSAYQGKTSISTVGTSTSAYRLSLATMSRSNTGTGTVWEQDSEPSQQASQDTLSRTDEEDEESWQKLHTPS
IRESfinder result
IRESfinder score
0.51773648
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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