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mmu_circUlk2_131
Basic information of circular RNA
PanCircBase ID:
mmu_circUlk2_131
mm39_Chr. Position ID:
chr11|61716667|61718510|-
Gene name:
Ulk2
Transcript:
ENSMUST00000004920.4
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
11,10
Exon/intron offsets:
0,1760
Genomic length:
1843
Exon/intron sizes in nt:
48,83
Flanking exon/introns:
chr11:61715553-61716667|chr11:61718510-61720217
Splice length:
131
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TATTCCCAGAGAAACATCACCTTACTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAATCAAAAGGATAGAATGGACTTTGAAGCATTTTTCAGCCATCCTTTCCTTGAGCAAGTTCCAGTTAAAAAAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGACTTTGAAGCATTTTTCAGCC
59.26
TTGATTTCTCTGAAGCAAACCCA
58.79
120
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGACTTTGAAGCATTTTTCAGC
58.63
TGATTTCTCTGAAGCAAACCCA
58.17
120
PCR template sequence:
GGATAGAATGGACTTTGAAGCATTTTTCAGCCATCCTTTCCTTGAGCAAGTTCCAGTTAAAAAATTATTCCCAGAGAAACATCACCTTACTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAATCAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
CCAGTTAAAAAATTATTCCCAGAGAA
siRNA sets
GGAATAATTTTTTAACTGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCULK2_131_ORF_1
0.5898
-0.21846836
0.024559725
189
ORF sequence:
ATGGACTTTGAAGCATTTTTCAGCCATCCTTTCCTTGAGCAAGTTCCAGTTAAAAAATTATTCCCAGAGAAACATCACCTTACTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAATCAAAAGGATAGAATGGACTTTGAAGCATTTTTCAGCCATCCTTTCCTTGAGCAAGTTCCAGTTAAAAAAT
Peptide sequence:
MDFEAFFSHPFLEQVPVKKLFPEKHHLTWLISFWVCFREIKRIEWTLKHFSAILSLSKFQLKN
IRESfinder result
IRESfinder score
0.938142448
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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