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mmu_circUhrf1bp1l_633
Basic information of circular RNA
PanCircBase ID:
mmu_circUhrf1bp1l_633
mm39_Chr. Position ID:
chr10|89609941|89618602|+
Gene name:
Uhrf1bp1l
Transcript:
ENSMUST00000020112.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
3,4,5,6,7
Exon/intron offsets:
0,1859,5755,7047,8490
Genomic length:
8661
Exon/intron sizes in nt:
39,92,19,01,41,171
Flanking exon/introns:
chr10:89608611-89609941|chr10:89618602-89622966
Splice length:
633
Other database IDs:
circAtlas: mmu-Uhrf1bp1l_0006
circBase: hsa_circ_0027850, circAtlas: hsa-UHRF1BP1L_0002, CIRCpedia: HSA_CIRCpedia_119278
Human Ortholog IDs:
Mature spliced Sequence:
ATTCCATGGACAAAACTGAAAACACAGCCTATCTGTTTGTCTCTAGATAAGGTGATAATGGAGATGAGTACATGTGAAGAGCCACGAGCCCCTAACGGTCCGTCACCGATTGCAACTGCCTCAGGACAGAGTGAGTACGGCTTTGCTGAGAAGGTTGTTGAGGGAATTACTGTTTCTGTCAACTCTATCGTCATCAGAATTGGAGCGAAAGCTTTCAATGCTTCCTTTGAGCTTTCTCAGCTCCGTATCTACAGTGTCAATGCACAGTGGGAGCACGGAGACCTGAGATTTACTCGGATTCAGGATCCGCAGAGAGGAGAGGTTTTGACATTTAAAGAAATAAATTGGCAGATGATTCGAATTGAGGCAGATGCTACCCAAAGTTCACATCTTGAAATTATGTGTGCTCCTGTTCGATTAATAACTAATCAGTCAAAAATCCGAGTCACCCTTAAAAGAAGATTAAAAGACTGCAATGTCATTGCAACAAAGTTAGTTCTAATTCTGGATGATTTGTTATGGGTTTTAACGGATTCCCAGTTGAAGGCTATGGTGCAATATGCAAAGTCTCTTAGTGAAGCAATAGAGAAATCAACGGAGCAAAGGAAGAGCATGGCTCCTGAACCTACTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACGGAGCAAAGGAAGAGCA
59.89
GACCGTTAGGGGCTCGTG
59.82
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAACGGAGCAAAGGAAGAGC
59.48
GACCGTTAGGGGCTCGTG
59.82
141
PCR template sequence:
TTCCCAGTTGAAGGCTATGGTGCAATATGCAAAGTCTCTTAGTGAAGCAATAGAGAAATCAACGGAGCAAAGGAAGAGCATGGCTCCTGAACCTACTCAGATTCCATGGACAAAACTGAAAACACAGCCTATCTGTTTGTCTCTAGATAAGGTGATAATGGAGATGAGTACATGTGAAGAGCCACGAGCCCCTAACGGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-137-3p(1), mmu-miR-709(1), mmu-miR-127-3p(1), mmu-miR-363-3p(1), mmu-miR-676-3p(1), mmu-miR-7a-1-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGAACCTACTCAGATTCCATGGACAA
siRNA sets
TTGTCCATGGAATCTGAGT, TCCATGGAATCTGAGTAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUHRF1BP1L_633_ORF_1
1.095
0.089049555
0.997134793
1209
ORF sequence:
ATGGAGATGAGTACATGTGAAGAGCCACGAGCCCCTAACGGTCCGTCACCGATTGCAACTGCCTCAGGACAGAGTGAGTACGGCTTTGCTGAGAAGGTTGTTGAGGGAATTACTGTTTCTGTCAACTCTATCGTCATCAGAATTGGAGCGAAAGCTTTCAATGCTTCCTTTGAGCTTTCTCAGCTCCGTATCTACAGTGTCAATGCACAGTGGGAGCACGGAGACCTGAGATTTACTCGGATTCAGGATCCGCAGAGAGGAGAGGTTTTGACATTTAAAGAAATAAATTGGCAGATGATTCGAATTGAGGCAGATGCTACCCAAAGTTCACATCTTGAAATTATGTGTGCTCCTGTTCGATTAATAACTAATCAGTCAAAAATCCGAGTCACCCTTAAAAGAAGATTAAAAGACTGCAATGTCATTGCAACAAAGTTAGTTCTAATTCTGGATGATTTGTTATGGGTTTTAACGGATTCCCAGTTGAAGGCTATGGTGCAATATGCAAAGTCTCTTAGTGAAGCAATAGAGAAATCAACGGAGCAAAGGAAGAGCATGGCTCCTGAACCTACTCAGATTCCATGGACAAAACTGAAAACACAGCCTATCTGTTTGTCTCTAGATAAGGTGATAATGGAGATGAGTACATGTGAAGAGCCACGAGCCCCTAACGGTCCGTCACCGATTGCAACTGCCTCAGGACAGAGTGAGTACGGCTTTGCTGAGAAGGTTGTTGAGGGAATTACTGTTTCTGTCAACTCTATCGTCATCAGAATTGGAGCGAAAGCTTTCAATGCTTCCTTTGAGCTTTCTCAGCTCCGTATCTACAGTGTCAATGCACAGTGGGAGCACGGAGACCTGAGATTTACTCGGATTCAGGATCCGCAGAGAGGAGAGGTTTTGACATTTAAAGAAATAAATTGGCAGATGATTCGAATTGAGGCAGATGCTACCCAAAGTTCACATCTTGAAATTATGTGTGCTCCTGTTCGATTAATAACTAATCAGTCAAAAATCCGAGTCACCCTTAAAAGAAGATTAAAAGACTGCAATGTCATTGCAACAAAGTTAGTTCTAATTCTGGATGATTTGTTATGGGTTTTAACGGATTCCCAGTTGAAGGCTATGGTGCAATATGCAAAGTCTCTTAGTGAAGCAATAGAGAAATCAACGGAGCAAAGGAAGAGCATGGCTCCTGAACCTACTCAG
Peptide sequence:
MEMSTCEEPRAPNGPSPIATASGQSEYGFAEKVVEGITVSVNSIVIRIGAKAFNASFELSQLRIYSVNAQWEHGDLRFTRIQDPQRGEVLTFKEINWQMIRIEADATQSSHLEIMCAPVRLITNQSKIRVTLKRRLKDCNVIATKLVLILDDLLWVLTDSQLKAMVQYAKSLSEAIEKSTEQRKSMAPEPTQIPWTKLKTQPICLSLDKVIMEMSTCEEPRAPNGPSPIATASGQSEYGFAEKVVEGITVSVNSIVIRIGAKAFNASFELSQLRIYSVNAQWEHGDLRFTRIQDPQRGEVLTFKEINWQMIRIEADATQSSHLEIMCAPVRLITNQSKIRVTLKRRLKDCNVIATKLVLILDDLLWVLTDSQLKAMVQYAKSLSEAIEKSTEQRKSMAPEPTQ
IRESfinder result
IRESfinder score
0.649802348
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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