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mmu_circUggt1_696
Basic information of circular RNA
PanCircBase ID:
mmu_circUggt1_696
mm39_Chr. Position ID:
chr1|36247022|36266695|-
Gene name:
Uggt1
Transcript:
ENSMUST00000046875.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
9,8,7,6,5,4
Exon/intron offsets:
0,2044,3448,8190,13270,19542
Genomic length:
19673
Exon/intron sizes in nt:
1,01,79,97,17,51,13,130
Flanking exon/introns:
chr1:36241959-36247022|chr1:36266695-36269738
Splice length:
696
Other database IDs:
CIRCpedia: MMU_CIRCpedia_18067, circAtlas: mmu-Uggt1_0033
Human Ortholog IDs:
Mature spliced Sequence:
ATACTGATCACTCCTATTACGATGCCGTATTGGAAGCTGCGTTTCGCTTCCTGTCCCCTCTGCAGCAGAATTTGTTGAAGTTTTGTCTCTCTCTCCGTTCCTACTCAGCCTCAATTCAAGCCTTCCAGCAGATAGCAGTTGACGAGCCTCCACCAGAAGGGTGCAAGTCGTTTCTCTCAGTGCATGGAAAGCAGACTTGTGATCTAGACACTCTGGAGAGCCTTCTGCTGACTGCCGCTGACAGACCGAAACCATTATTGTTCAAAGGAGATCACAGATATCCCTCATCAAATCCTGAGAGTCCAGTGGTCATCCTTTATTCTGAGATTGGCCATGAAGAATTTTCTAATATTCACCACCAACTTATATCAAAAAGCAACGAAGGCAAAATTAATTATGTATTCCGACATTATATATCTAATCCCAGCAAGGAGCCGGTTTACCTTTCTGGCTATGGAGTGGAACTGGCGATCAAGAGCACGGAGTACAAGGCCAAGGATGATACTCAGGTGAAAGGAACTGAGGTAAATGCCACGGTCATCGGGGAGAGCGACCCTATTGATGAAGTGCAGGGATTCCTCTTTGGAAAATTAAGAGAACTGTACCCCGCCTTGGAAGGACAGCTGAAAGAGTTCCGGAAGCACCTTGTGGAGAGCACCAACGAGATGGCCCCCCTGAAAGTCTGGCAGCTGCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGTTCCGGAAGCACCTTG
59.96
AATTCTGCTGCAGAGGGGAC
60.03
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGTTCCGGAAGCACCTTG
59.96
AAATTCTGCTGCAGAGGGGA
59.3
141
PCR template sequence:
GAACTGTACCCCGCCTTGGAAGGACAGCTGAAAGAGTTCCGGAAGCACCTTGTGGAGAGCACCAACGAGATGGCCCCCCTGAAAGTCTGGCAGCTGCAAGATACTGATCACTCCTATTACGATGCCGTATTGGAAGCTGCGTTTCGCTTCCTGTCCCCTCTGCAGCAGAATTTGTTGAAGTTTTGTCTCTCTCTCCGTTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-18a-5p(1), mmu-miR-185-5p(1), mmu-miR-22-3p(1), mmu-miR-3065-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
TGGCAGCTGCAAGATACTGATCACTC
siRNA sets
AGTGATCAGTATCTTGCAG, GTGATCAGTATCTTGCAGC, GAGTGATCAGTATCTTGCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCUGGT1_696_ORF_1
1.0988
0.242628788
0.952519473
726
ORF sequence:
ATGGCCCCCCTGAAAGTCTGGCAGCTGCAAGATACTGATCACTCCTATTACGATGCCGTATTGGAAGCTGCGTTTCGCTTCCTGTCCCCTCTGCAGCAGAATTTGTTGAAGTTTTGTCTCTCTCTCCGTTCCTACTCAGCCTCAATTCAAGCCTTCCAGCAGATAGCAGTTGACGAGCCTCCACCAGAAGGGTGCAAGTCGTTTCTCTCAGTGCATGGAAAGCAGACTTGTGATCTAGACACTCTGGAGAGCCTTCTGCTGACTGCCGCTGACAGACCGAAACCATTATTGTTCAAAGGAGATCACAGATATCCCTCATCAAATCCTGAGAGTCCAGTGGTCATCCTTTATTCTGAGATTGGCCATGAAGAATTTTCTAATATTCACCACCAACTTATATCAAAAAGCAACGAAGGCAAAATTAATTATGTATTCCGACATTATATATCTAATCCCAGCAAGGAGCCGGTTTACCTTTCTGGCTATGGAGTGGAACTGGCGATCAAGAGCACGGAGTACAAGGCCAAGGATGATACTCAGGTGAAAGGAACTGAGGTAAATGCCACGGTCATCGGGGAGAGCGACCCTATTGATGAAGTGCAGGGATTCCTCTTTGGAAAATTAAGAGAACTGTACCCCGCCTTGGAAGGACAGCTGAAAGAGTTCCGGAAGCACCTTGTGGAGAGCACCAACGAGATGGCCCCCCTGAAAGTCTGGCAGCTGCAA
Peptide sequence:
MAPLKVWQLQDTDHSYYDAVLEAAFRFLSPLQQNLLKFCLSLRSYSASIQAFQQIAVDEPPPEGCKSFLSVHGKQTCDLDTLESLLLTAADRPKPLLFKGDHRYPSSNPESPVVILYSEIGHEEFSNIHHQLISKSNEGKINYVFRHYISNPSKEPVYLSGYGVELAIKSTEYKAKDDTQVKGTEVNATVIGESDPIDEVQGFLFGKLRELYPALEGQLKEFRKHLVESTNEMAPLKVWQLQ
IRESfinder result
IRESfinder score
0.570227668
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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