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mmu_circTtyh2_390
Basic information of circular RNA
PanCircBase ID:
mmu_circTtyh2_390
mm39_Chr. Position ID:
chr11|114587414|114593134|+
Gene name:
Ttyh2
Transcript:
ENSMUST00000045779.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,5,6
Exon/intron offsets:
0,5192,5647
Genomic length:
5720
Exon/intron sizes in nt:
221,96,73
Flanking exon/introns:
chr11:114581176-114587414|chr11:114593134-114598161
Splice length:
390
Other database IDs:
circAtlas: mmu-Ttyh2_0011
Human Ortholog IDs:
Mature spliced Sequence:
GTGTCTGCAAACACCCAGAGGATGAAGGTAGACCTAGAACAGCACCTGGCCCGGCTCAGCGAGATCATTGCTGCCCGGGGTGACTACATCCAGACCCTGAAGTTTATGCAACAGATGGCAGGCAATGTCGTCAGCCAGCTCTCGGGGCTGCCCGTGTGGAGGGAGGTCACCACGCAGCTGACCAAGCTGTCCCACCAGACTGCCTATGTGGAATACTACAGGTGGCTGTCCTACCTCCTGCTTTTCATCCTTGACCTGGTCATCTGCCTTGTCACCTGCCTGGGACTGGCCAGGCGGTCCAAGTGTCTCCTAGCCTCCATGCTGTGCTGTGGAATACTGACCCTGATCCTCAGCTGGGCTTCTCTGGCTGCTGATGCTGCTGCAGCAGTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCCTAGCCTCCATGCTGT
60.03
GTCTGGATGTAGTCACCCCG
59.54
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCCATGCTGTGCTGTGGAAT
59.96
GTCTGGATGTAGTCACCCCG
59.54
170
PCR template sequence:
CAGGCGGTCCAAGTGTCTCCTAGCCTCCATGCTGTGCTGTGGAATACTGACCCTGATCCTCAGCTGGGCTTCTCTGGCTGCTGATGCTGCTGCAGCAGTGGTGTCTGCAAACACCCAGAGGATGAAGGTAGACCTAGAACAGCACCTGGCCCGGCTCAGCGAGATCATTGCTGCCCGGGGTGACTACATCCAGACCCTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-205-5p(1), mmu-let-7c-5p(1), mmu-miR-107-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TGCTGCAGCAGTGGTGTCTGCAAACA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTTYH2_390_ORF_1
1.323
0.373204693
0.985255663
759
ORF sequence:
ATGAAGGTAGACCTAGAACAGCACCTGGCCCGGCTCAGCGAGATCATTGCTGCCCGGGGTGACTACATCCAGACCCTGAAGTTTATGCAACAGATGGCAGGCAATGTCGTCAGCCAGCTCTCGGGGCTGCCCGTGTGGAGGGAGGTCACCACGCAGCTGACCAAGCTGTCCCACCAGACTGCCTATGTGGAATACTACAGGTGGCTGTCCTACCTCCTGCTTTTCATCCTTGACCTGGTCATCTGCCTTGTCACCTGCCTGGGACTGGCCAGGCGGTCCAAGTGTCTCCTAGCCTCCATGCTGTGCTGTGGAATACTGACCCTGATCCTCAGCTGGGCTTCTCTGGCTGCTGATGCTGCTGCAGCAGTGGTGTCTGCAAACACCCAGAGGATGAAGGTAGACCTAGAACAGCACCTGGCCCGGCTCAGCGAGATCATTGCTGCCCGGGGTGACTACATCCAGACCCTGAAGTTTATGCAACAGATGGCAGGCAATGTCGTCAGCCAGCTCTCGGGGCTGCCCGTGTGGAGGGAGGTCACCACGCAGCTGACCAAGCTGTCCCACCAGACTGCCTATGTGGAATACTACAGGTGGCTGTCCTACCTCCTGCTTTTCATCCTTGACCTGGTCATCTGCCTTGTCACCTGCCTGGGACTGGCCAGGCGGTCCAAGTGTCTCCTAGCCTCCATGCTGTGCTGTGGAATACTGACCCTGATCCTCAGCTGGGCTTCTCTGGCTGCTGATGCTGCTGCAGCAGTG
Peptide sequence:
MKVDLEQHLARLSEIIAARGDYIQTLKFMQQMAGNVVSQLSGLPVWREVTTQLTKLSHQTAYVEYYRWLSYLLLFILDLVICLVTCLGLARRSKCLLASMLCCGILTLILSWASLAADAAAAVVSANTQRMKVDLEQHLARLSEIIAARGDYIQTLKFMQQMAGNVVSQLSGLPVWREVTTQLTKLSHQTAYVEYYRWLSYLLLFILDLVICLVTCLGLARRSKCLLASMLCCGILTLILSWASLAADAAAAV
IRESfinder result
IRESfinder score
0.460346911
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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