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mmu_circTtc27_741
Basic information of circular RNA
PanCircBase ID:
mmu_circTtc27_741
mm39_Chr. Position ID:
chr17|75054646|75106406|+
Gene name:
Ttc27
Transcript:
ENSMUST00000024882.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
8,9,10,11,12,13
Exon/intron offsets:
0,3410,22591,29992,33131,51532
Genomic length:
51760
Exon/intron sizes in nt:
1,13,67,11,49,61,23,220
Flanking exon/introns:
chr17:75050046-75054646|chr17:75106406-75136486
Splice length:
741
Other database IDs:
circAtlas: mmu-Ttc27_0035
Human Ortholog IDs:
Mature spliced Sequence:
AATCTTGAGCTCAATGATGATACTGTTCTGAATGAAATAAAGTTAGCAGATAGTGAACGGTTTCAGATGCCAGACCTGTGTGCGGAGGAGCTTGCCGTGGTCCTTGGAGTCTGCACAAATTTCCAGAAGAACAACCCAGTTCATAAGCTAACGGAGGAGGAGCTGCTGGCCTTCACATCGTGTTTACTTTCACAACCAAAGTTCTGGGCCATTCAGATGTCAGCCTTGATCCTCCGGACAAAACTTGAGAGAGGAAGCACACGGCGAGTGGAGCGGGCCATGAGGCAGACCCAGGCTCTTGCAGACCAATTCGAAGATAAAGCCACATCTGTACTGGAACGTCTGAAGATTTTCTATTGTTGTCAAGTACCCCCTCACTGGGCTGTTCAGCGCCAGCTCGCAGGTTTGCTGTTTGAGTTGGGATGCACCTCCTCAGCCCTGCAGATATTTGAGAAGCTGGAGATGTGGGAGGATGTGGTCATCTGCCACGAGAGGGCTGGGCGGCACGGGAAGGCAGAAGAAATACTTAGGCAAGAACTGGAGAAAAAGGAAACACCAGGTTTATATTGCTTACTTGGAGATGTCCTGCAGGACCATTCCTGCTACGACAAGGCCTGGGAGCTGTCCCGGCATCGCAGTGCACGTGCCCAGCGCTCCAAAGCTCTCCTCCATCTTCGGAACAAGGAGTTTCGGGAATGTGTGGAGTGCTTCGAGCGCTCAGTGAAGATCAATCCCATGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCTCTCCTCCATCTTCGGA
59.74
AGGTCTGGCATCTGAAACCG
60.04
159
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCCAAAGCTCTCCTCCAT
59.45
AGGTCTGGCATCTGAAACCG
60.04
166
PCR template sequence:
ACGTGCCCAGCGCTCCAAAGCTCTCCTCCATCTTCGGAACAAGGAGTTTCGGGAATGTGTGGAGTGCTTCGAGCGCTCAGTGAAGATCAATCCCATGCAGAATCTTGAGCTCAATGATGATACTGTTCTGAATGAAATAAAGTTAGCAGATAGTGAACGGTTTCAGATGCCAGACCTGTGTGCGGAGGAGCTTGCCGTGG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-9-5p(1), mmu-miR-431-5p(1), mmu-miR-182-5p(1), mmu-miR-218-5p(1), mmu-miR-188-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CAATCCCATGCAGAATCTTGAGCTCA
siRNA sets
AGCTCAAGATTCTGCATGG, GCTCAAGATTCTGCATGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTTC27_741_ORF_1
1.0835
0.282789534
0.999700082
1416
ORF sequence:
ATGCCAGACCTGTGTGCGGAGGAGCTTGCCGTGGTCCTTGGAGTCTGCACAAATTTCCAGAAGAACAACCCAGTTCATAAGCTAACGGAGGAGGAGCTGCTGGCCTTCACATCGTGTTTACTTTCACAACCAAAGTTCTGGGCCATTCAGATGTCAGCCTTGATCCTCCGGACAAAACTTGAGAGAGGAAGCACACGGCGAGTGGAGCGGGCCATGAGGCAGACCCAGGCTCTTGCAGACCAATTCGAAGATAAAGCCACATCTGTACTGGAACGTCTGAAGATTTTCTATTGTTGTCAAGTACCCCCTCACTGGGCTGTTCAGCGCCAGCTCGCAGGTTTGCTGTTTGAGTTGGGATGCACCTCCTCAGCCCTGCAGATATTTGAGAAGCTGGAGATGTGGGAGGATGTGGTCATCTGCCACGAGAGGGCTGGGCGGCACGGGAAGGCAGAAGAAATACTTAGGCAAGAACTGGAGAAAAAGGAAACACCAGGTTTATATTGCTTACTTGGAGATGTCCTGCAGGACCATTCCTGCTACGACAAGGCCTGGGAGCTGTCCCGGCATCGCAGTGCACGTGCCCAGCGCTCCAAAGCTCTCCTCCATCTTCGGAACAAGGAGTTTCGGGAATGTGTGGAGTGCTTCGAGCGCTCAGTGAAGATCAATCCCATGCAGAATCTTGAGCTCAATGATGATACTGTTCTGAATGAAATAAAGTTAGCAGATAGTGAACGGTTTCAGATGCCAGACCTGTGTGCGGAGGAGCTTGCCGTGGTCCTTGGAGTCTGCACAAATTTCCAGAAGAACAACCCAGTTCATAAGCTAACGGAGGAGGAGCTGCTGGCCTTCACATCGTGTTTACTTTCACAACCAAAGTTCTGGGCCATTCAGATGTCAGCCTTGATCCTCCGGACAAAACTTGAGAGAGGAAGCACACGGCGAGTGGAGCGGGCCATGAGGCAGACCCAGGCTCTTGCAGACCAATTCGAAGATAAAGCCACATCTGTACTGGAACGTCTGAAGATTTTCTATTGTTGTCAAGTACCCCCTCACTGGGCTGTTCAGCGCCAGCTCGCAGGTTTGCTGTTTGAGTTGGGATGCACCTCCTCAGCCCTGCAGATATTTGAGAAGCTGGAGATGTGGGAGGATGTGGTCATCTGCCACGAGAGGGCTGGGCGGCACGGGAAGGCAGAAGAAATACTTAGGCAAGAACTGGAGAAAAAGGAAACACCAGGTTTATATTGCTTACTTGGAGATGTCCTGCAGGACCATTCCTGCTACGACAAGGCCTGGGAGCTGTCCCGGCATCGCAGTGCACGTGCCCAGCGCTCCAAAGCTCTCCTCCATCTTCGGAACAAGGAGTTTCGGGAATGTGTGGAGTGCTTCGAGCGCTCAGTGAAGATCAATCCCATGCAG
Peptide sequence:
MPDLCAEELAVVLGVCTNFQKNNPVHKLTEEELLAFTSCLLSQPKFWAIQMSALILRTKLERGSTRRVERAMRQTQALADQFEDKATSVLERLKIFYCCQVPPHWAVQRQLAGLLFELGCTSSALQIFEKLEMWEDVVICHERAGRHGKAEEILRQELEKKETPGLYCLLGDVLQDHSCYDKAWELSRHRSARAQRSKALLHLRNKEFRECVECFERSVKINPMQNLELNDDTVLNEIKLADSERFQMPDLCAEELAVVLGVCTNFQKNNPVHKLTEEELLAFTSCLLSQPKFWAIQMSALILRTKLERGSTRRVERAMRQTQALADQFEDKATSVLERLKIFYCCQVPPHWAVQRQLAGLLFELGCTSSALQIFEKLEMWEDVVICHERAGRHGKAEEILRQELEKKETPGLYCLLGDVLQDHSCYDKAWELSRHRSARAQRSKALLHLRNKEFRECVECFERSVKINPMQ
IRESfinder result
IRESfinder score
0.376779863
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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