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mmu_circTtbk2_1338
Basic information of circular RNA
PanCircBase ID:
mmu_circTtbk2_1338
mm39_Chr. Position ID:
chr2|120586310|120655796|-
Gene name:
Ttbk2
Transcript:
ENSMUST00000085840.11
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
11
Exon/intron indexes:
12,11,10,9,8,7,6,5,4,3,2
Exon/intron offsets:
0,4367,17399,17990,21704,27865,30149,34452,50929,66643,69412
Genomic length:
69486
Exon/intron sizes in nt:
217,158,126,93,66,105,141,74,148,136,74
Flanking exon/introns:
chr2:120580930-120586310|chr2:120655796-120680536
Splice length:
1338
Other database IDs:
CIRCpedia: MMU_CIRCpedia_5194, circAtlas: mmu-Ttbk2_0009
Human Ortholog IDs:
Mature spliced Sequence:
AATTGTAAAACCGGTAAGATCAAGTGCCTATTGAAAGAGACTCACTCTTGGCTAAGAAGAGAAATAGGAAATAGGTTAAATGGAATCCACCCTTGGGAGCTAGATGCCTGTGTAGCTGTTTTACCTCATCAGGGTTTTGCAATGAGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGTTAAGAAAGATTGGAGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGTGGCGCTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAACTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGAAATGATCGTTTCAACTACGTGGTCATGCAATTGCAGGGACGGAATCTGGCAGATCTCCGCCGTAGCCAATCCCGGGGCACATTCACTATTAGCACTACCCTTCGTCTTGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGAGACATCAAACCGTCAAACTTCGCCATGGGACGTTTCCCCAGTACATGTAGGAAATGTTTCATGCTTGATTTTGGCTTGGCTCGACAATTTACTAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTTCGAGGGACAGTTCGTTATGCATCAATCAATGCTCATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCTTTATTCTACATGTTGGTGGAGTTTGTGGTTGGCCAACTGCCTTGGAGAAAAATAAAGGACAAGGAGCAAGTAGGCTCCATTAAGGAGAGATATGACCACAGGCTCATGTTAAAACACCTCCCTCCAGAATTCAGCACCTTTCTTGACCATATTTCCTCTTTGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTTGACAATAGCATCAAGACCTTTGGAGTAATTGAGAGTGACCCGTTTGACTGGGAGAAGAGTGGAACTGATGGCTCCCTGACAACCACCACCACCTCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCTATCGGAATTGCAAATGCCACCCCCATCCCAGGAGACTTGCTTCGAGAAAATACAGATGAAGTGTTTCCAGATGAACAGCTTAGTGATGGGGAGAACGGAATCCCTGTTGGTGTATCACCAGATAAATTGCCTGGATCTCTGGGGCACCCACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAATTTGCAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGCCCTCAGGAAAAGGATGT
60.04
CAAGGGTGGATTCCATTTAACCT
58.65
167
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACGCCCTCAGGAAAAGGATG
60.04
CAAGGGTGGATTCCATTTAACCT
58.65
168
PCR template sequence:
TAAATTGCCTGGATCTCTGGGGCACCCACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAATTTGCAAAAATTGTAAAACCGGTAAGATCAAGTGCCTATTGAAAGAGACTCACTCTTGGCTAAGAAGAGAAATAGGAAATAGGTTAAATGGAATCCACCCTTGGGAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-let-7e-5p(1), mmu-miR-298-5p(1), mmu-let-7d-5p(1), mmu-miR-541-5p(1), mmu-miR-680(1), mmu-miR-697(1), mmu-miR-542-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GGGAATTTGCAAAAATTGTAAAACCG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTTBK2_1338_ORF_1
0.9386
0.179043862
0.999999808
2535
ORF sequence:
ATGAGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGTTAAGAAAGATTGGAGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGTGGCGCTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAACTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGAAATGATCGTTTCAACTACGTGGTCATGCAATTGCAGGGACGGAATCTGGCAGATCTCCGCCGTAGCCAATCCCGGGGCACATTCACTATTAGCACTACCCTTCGTCTTGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGAGACATCAAACCGTCAAACTTCGCCATGGGACGTTTCCCCAGTACATGTAGGAAATGTTTCATGCTTGATTTTGGCTTGGCTCGACAATTTACTAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTTCGAGGGACAGTTCGTTATGCATCAATCAATGCTCATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCTTTATTCTACATGTTGGTGGAGTTTGTGGTTGGCCAACTGCCTTGGAGAAAAATAAAGGACAAGGAGCAAGTAGGCTCCATTAAGGAGAGATATGACCACAGGCTCATGTTAAAACACCTCCCTCCAGAATTCAGCACCTTTCTTGACCATATTTCCTCTTTGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTTGACAATAGCATCAAGACCTTTGGAGTAATTGAGAGTGACCCGTTTGACTGGGAGAAGAGTGGAACTGATGGCTCCCTGACAACCACCACCACCTCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCTATCGGAATTGCAAATGCCACCCCCATCCCAGGAGACTTGCTTCGAGAAAATACAGATGAAGTGTTTCCAGATGAACAGCTTAGTGATGGGGAGAACGGAATCCCTGTTGGTGTATCACCAGATAAATTGCCTGGATCTCTGGGGCACCCACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAATTTGCAAAAATTGTAAAACCGGTAAGATCAAGTGCCTATTGAAAGAGACTCACTCTTGGCTAAGAAGAGAAATAGGAAATAGGTTAAATGGAATCCACCCTTGGGAGCTAGATGCCTGTGTAGCTGTTTTACCTCATCAGGGTTTTGCAATGAGTGGAGGAGGAGAGCAGCCAGATATCCTCAGTGTTGGAATCCTGGTCAAAGAAAGATGGAAAGTGTTAAGAAAGATTGGAGGTGGGGGCTTTGGAGAAATTTACGATGCCTTGGACATGCTCACCAGGGAGAATGTGGCGCTGAAGGTGGAGTCAGCTCAGCAGCCAAAGCAGGTTCTGAAGATGGAGGTTGCTGTGTTGAAGAAACTGCAAGGGAAAGACCATGTTTGTAGATTTATTGGCTGTGGGAGAAATGATCGTTTCAACTACGTGGTCATGCAATTGCAGGGACGGAATCTGGCAGATCTCCGCCGTAGCCAATCCCGGGGCACATTCACTATTAGCACTACCCTTCGTCTTGGGAAACAGATTCTGGAGTCTATTGAAAGCATACATTCTGTGGGATTCCTTCACAGAGACATCAAACCGTCAAACTTCGCCATGGGACGTTTCCCCAGTACATGTAGGAAATGTTTCATGCTTGATTTTGGCTTGGCTCGACAATTTACTAATTCCTGTGGTGACGTCAGACCACCTCGTGCTGTGGCAGGCTTTCGAGGGACAGTTCGTTATGCATCAATCAATGCTCATCGGAACAGGGAAATGGGAAGACATGATGACCTTTGGTCTTTATTCTACATGTTGGTGGAGTTTGTGGTTGGCCAACTGCCTTGGAGAAAAATAAAGGACAAGGAGCAAGTAGGCTCCATTAAGGAGAGATATGACCACAGGCTCATGTTAAAACACCTCCCTCCAGAATTCAGCACCTTTCTTGACCATATTTCCTCTTTGGATTATTTTACAAAACCGGACTACCAGCTTCTAACATCCGTGTTTGACAATAGCATCAAGACCTTTGGAGTAATTGAGAGTGACCCGTTTGACTGGGAGAAGAGTGGAACTGATGGCTCCCTGACAACCACCACCACCTCTGCCACCCCTCAGCTGCACACCCGCCTGACCCCTGCTGCTATCGGAATTGCAAATGCCACCCCCATCCCAGGAGACTTGCTTCGAGAAAATACAGATGAAGTGTTTCCAGATGAACAGCTTAGTGATGGGGAGAACGGAATCCCTGTTGGTGTATCACCAGATAAATTGCCTGGATCTCTGGGGCACCCACGCCCTCAGGAAAAGGATGTCTGGGAAGAGATGGATATCAACAAGAACAAGATAAAGCTGGGAATTTGCAAA
Peptide sequence:
MSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGKQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCFMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSTFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKSGTDGSLTTTTTSATPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDINKNKIKLGICKNCKTGKIKCLLKETHSWLRREIGNRLNGIHPWELDACVAVLPHQGFAMSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGKQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCFMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSTFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKSGTDGSLTTTTTSATPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSDGENGIPVGVSPDKLPGSLGHPRPQEKDVWEEMDINKNKIKLGICK
IRESfinder result
IRESfinder score
0.588570708
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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