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mmu_circTrrap_745
Basic information of circular RNA
PanCircBase ID:
mmu_circTrrap_745
mm39_Chr. Position ID:
chr5|144756511|144761413|+
Gene name:
Trrap
Transcript:
ENSMUST00000100467.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
42,43,44,45,46
Exon/intron offsets:
0,546,2071,2989,4755
Genomic length:
4902
Exon/intron sizes in nt:
11,11,47,21,41,26,147
Flanking exon/introns:
chr5:144755130-144756511|chr5:144761413-144762028
Splice length:
745
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGTTCGGGAGGAGCCAGTCCCTGCCGGGAGCTGACTCTCTTCTTGCCAAGCCCATTGACAAGCAGCACACAGACACTGTGGTGAACTTCCTCATTCGAGTGGCTTGCCAGGTGAACGACAACACCAACACGGCAGGGTCGCCTGGGGAGGTGCTCTCTCGCCGCTGTGTGAATCTTCTCAAGACCGCTCTACGGCCAGATATGTGGTGCAAGTCTGAGCTCAAACTGCAGTGGTTTGACAAGCTGCTGATGACCGTGGAGCAGCCTAACCAGGTGAACTATGGGAATATCTGCACAGGGTTGGAAGTCCTGAACTTCTTGCTAACTGTGCTGCAGTCTCCGGCCATCCTCAGTAGCTTCAAGCCCCTGCAGCGGGGCATCGCAGCCTGCATGACATGTGGGAACACCAAGGTGCTGCGAGCTGTGCACAGCCTCCTCTCTCGCCTCATGAGCATTTTCCCTACAGAGCCAAGCACATCAAGCGTGGCCTCCAAGTATGAGGAGCTGGAGTGCCTCTATGCGGCCGTGGGGAAGGTCATCTACGAAGGCCTCACTAACTACGAGAAGGCCACCAGCGCCAACCCCTCCCAGCTCTTCGGAACACTTATGATCCTCAAGTCAGCCTGTTGCAACAACCCAAGCTACATTGACAGGCTCATCTCTGTCTTCATGCGCTCCCTACAGAAGATGGTCCGAGAGCATCTAAATCCACAGACAGCATCCGGGAGCACCGAAGCAACCGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCTGTCTTCATGCGCTCC
59.83
TGGGCTTGGCAAGAAGAGAG
59.96
142
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TCTCTGTCTTCATGCGCTCC
59.83
GGGCTTGGCAAGAAGAGAGT
59.96
141
PCR template sequence:
ATTGACAGGCTCATCTCTGTCTTCATGCGCTCCCTACAGAAGATGGTCCGAGAGCATCTAAATCCACAGACAGCATCCGGGAGCACCGAAGCAACCGCAGGTGTTCGGGAGGAGCCAGTCCCTGCCGGGAGCTGACTCTCTTCTTGCCAAGCCCATTGACAAGCAGCACACAGACACTGTGGTGAACTTCCTCATTCGAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-20b-5p(1), mmu-miR-143-3p(1), mmu-miR-127-5p(1), mmu-miR-182-5p(1), mmu-miR-377-3p(1), mmu-miR-673-5p(1), mmu-miR-188-3p(1), mmu-miR-376a-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GAAGCAACCGCAGGTGTTCGGGAGGA
siRNA sets
TCCTCCCGAACACCTGCGG, GAACACCTGCGGTTGCTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTRRAP_745_ORF_1
1.2164
0.40116468
0.942815121
579
ORF sequence:
ATGTGGTGCAAGTCTGAGCTCAAACTGCAGTGGTTTGACAAGCTGCTGATGACCGTGGAGCAGCCTAACCAGGTGAACTATGGGAATATCTGCACAGGGTTGGAAGTCCTGAACTTCTTGCTAACTGTGCTGCAGTCTCCGGCCATCCTCAGTAGCTTCAAGCCCCTGCAGCGGGGCATCGCAGCCTGCATGACATGTGGGAACACCAAGGTGCTGCGAGCTGTGCACAGCCTCCTCTCTCGCCTCATGAGCATTTTCCCTACAGAGCCAAGCACATCAAGCGTGGCCTCCAAGTATGAGGAGCTGGAGTGCCTCTATGCGGCCGTGGGGAAGGTCATCTACGAAGGCCTCACTAACTACGAGAAGGCCACCAGCGCCAACCCCTCCCAGCTCTTCGGAACACTTATGATCCTCAAGTCAGCCTGTTGCAACAACCCAAGCTACATTGACAGGCTCATCTCTGTCTTCATGCGCTCCCTACAGAAGATGGTCCGAGAGCATCTAAATCCACAGACAGCATCCGGGAGCACCGAAGCAACCGCAGGTGTTCGGGAGGAGCCAGTCCCTGCCGGGAGCTGA
Peptide sequence:
MWCKSELKLQWFDKLLMTVEQPNQVNYGNICTGLEVLNFLLTVLQSPAILSSFKPLQRGIAACMTCGNTKVLRAVHSLLSRLMSIFPTEPSTSSVASKYEELECLYAAVGKVIYEGLTNYEKATSANPSQLFGTLMILKSACCNNPSYIDRLISVFMRSLQKMVREHLNPQTASGSTEATAGVREEPVPAGS
IRESfinder result
IRESfinder score
0.368125876
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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