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mmu_circTrpm7_2686
Basic information of circular RNA
PanCircBase ID:
mmu_circTrpm7_2686
mm39_Chr. Position ID:
chr2|126654615|126672183|-
Gene name:
Trpm7
Transcript:
ENSMUST00000103224.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
12
Exon/intron indexes:
26,25,24,23,22,21,20,19,18,17,16,15
Exon/intron offsets:
0,1992,4064,7141,8468,9774,11280,12716,12938,14019,16314,17433
Genomic length:
17568
Exon/intron sizes in nt:
7,13,13,31,85,12,71,75,00,00,00,00,00,00,00,00,00,000
Flanking exon/introns:
chr2:126652150-126654615|chr2:126672183-126673116
Splice length:
2686
Other database IDs:
circAtlas: mmu-Trpm7_0068
Human Ortholog IDs:
Mature spliced Sequence:
AGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAGAGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAGCCCAACCCTACAGACCAAAGATGGATGCATCTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAATTGTAGATATAGATGATCCAGAGACCAAGCGCTTTCCTTATCCTCTTAATGAATTATTAATTTGGGCTTGCCTTATGAAGAGGCAGGTCATGGCCCGCTTTTTATGGCAGCATGGTGAAGAATCAATGGCTAAAGCATTAGTTGCCTGTAAAATCTATCGTTCAATGGCTTATGAGGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGAGGAACTGAAGCAGTATTCCAATGATTTTGGCCAACTGGCAGTTGAATTACTGGAACAGTCCTTCAGACAGGATGAAACGATGGCTATGAAATTACTCACTTATGAACTCAAAAACTGGAGTAATTCAACCTGCCTCAAGTTAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACTTGTACACAGATGTTGTTATCTGATATGTGGATGGGACGGCTGAATATGAGAAAAAATTCCTGGTATAAGGTCATATTAAGCATTTTAGTTCCACCTGCCATATTAATGCTAGAGTATAAAACCAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAAATGACGATGGAGGATAGTGAAAACAATTTTCACAACATAACAGAAGAGATACCCATGGAAGTATTTAAAGAAGTAAAGATTTTGGACAGCAGTGATGGAAAGAATGAAATGGAGATACATATTAAATCAAAAAAGCTTCCAATCACACGAAAATTTTATGCCTTTTATCATGCACCAATTGTAAAGTTCTGGTTTAACACATTGGCATATTTAGGATTTCTGATGCTTTATACATTTGTAGTTCTTGTAAAAATGGAACAGTTACCTTCAGTTCAAGAATGGATTGTTATCGCTTATATTTTTACCTATGCTATTGAAAAAGTCCGTGAGGTCTTCATGTCTGAAGCTGGGAAAATCAGCCAGAAGATTAAAGTATGGTTTAGTGACTACTTCAATGTCAGTGACACAATTGCCATCATTTCTTTCTTTGTTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTATATTAATGCATATGATAATCATGTTTTTGTGGCTGGAAGATTAATTTACTGTCTTAATATAATATTTTGGTATGTGCGTTTGCTAGACTTTCTAGCCGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTGTAGTGATAATGGCTCTTGTATTGCTTAGTTTTGGTGTTCCCAGAAAAGCAATACTTTATCCACATGAAGAACCATCTTGGTCTCTTGCTAAAGATATAGTTTTTCATCCATACTGGATGATTTTTGGTGAAGTTTATGCATATGAAATTGATGTGTGTGCAAATGACTCCACTCTCCCGACAATCTGTGGTCCTGGAACTTGGTTGACTCCATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTCCTTATCGCATTTTTCAATAATGTATATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTATCAGCGGTATCATTTTATTATGGCTTATCATGAAAAACCAGTCCTGCCTCCTCCTCTTATCATCCTCAGCCATATAGTTTCACTGTTTTGCTGTGTATGCAAAAGAAGAAAGAAAGATAAGACTTCCGATGGGCCAAAACTTTTCTTAACAGAAGAAGATCAAAAGAAACTCCATGATTTTGAAGAGCAGTGTGTTGAGATGTACTTTGATGAGAAAGATGACAAATTCAATTCTGGGAGTGAAGAGAGAATCCGGGTCACTTTTGAAAGAGTGGAGCAGATGAGCATTCAGATTAAAGAAGTTGGAGATCGTGTCAACTACATAAAAAGATCATTACAGTCTTTAGATTCTCAAATTGGTCATCTGCAAGATCTCTCAGCCCTAACAGTAGATACATTGAAAACACTTACAGCCCAGAAAGCTTCAGAAGCTAGTAAAGTGCACAATGAGATCACACGAGAATTGAGTATTTCCAAACACTTGGCTCAGAATCTTATTGATGATGTTCCTGTAAGACCTTTGTGGAAGAAACCTAGTGCTGTAAACACACTGAGTTCCTCTCTTCCTCAAGGTGATCGGGAAAGTAATAATCCTTTTCTTTGTAATATTTTTATGAAAGATGAAAAAGACCCCCAATATAATCTGTTTGGACAAGATTTGCCCGTGATACCCCAGAGAAAAGAATTCAACATTCCAGAGGCTGGTTCCTCCTGTGGTGCCTTATTCCCAAGTGCTGTTTCTCCCCCAGAATTACGACAGAGACGACATGGGGTAGAAATGTTAAAAATATTTAATAAAAATCAAAAATTAGGCAGTTCACCTAATAGTTCACCACATATGTCCTCCCCACCAACCAAATTTTCTGTGAGTACCCCATCCCAGCCAAGTTGCAAAAGTCACTTGGAATCCACAACCAAAGATCAAGAACCCATTTTCTATAAAGCTGCAGAAGGGGATAACATAGAATTTGGAGCATTTGTGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCAAAAGTCACTTGGAATCCACA
59.93
CTCTTTCGCAACTGAGGGGT
59.96
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCAAAAGTCACTTGGAATCCACA
59.93
ACTCTTTCGCAACTGAGGGG
59.96
141
PCR template sequence:
CCAAGTTGCAAAAGTCACTTGGAATCCACAACCAAAGATCAAGAACCCATTTTCTATAAAGCTGCAGAAGGGGATAACATAGAATTTGGAGCATTTGTGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAGAGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
16
Interacting miRNAs (#binding sites)
mmu-miR-335-3p(3), mmu-miR-136-5p(2), mmu-let-7a-5p(1), mmu-let-7e-5p(1), mmu-miR-335-5p(1), mmu-miR-203-3p(1), mmu-miR-381-3p(1), mmu-let-7d-5p(1), mmu-miR-433-3p(1), mmu-miR-431-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-22-3p(1), mmu-miR-127-5p(1), mmu-miR-182-5p(1), mmu-miR-340-5p(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGAGCATTTGTGGAGGTCAGGTCGAA
siRNA sets
TGACCTCCACAAATGCTCC, CTGACCTCCACAAATGCTC, ACCTGACCTCCACAAATGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTRPM7_2686_ORF_1
0.8409
-0.028940516
0.999999753
2652
ORF sequence:
ATGAGACACAATCATTTCATTAAAACAGCCCAACCCTACAGACCAAAGATGGATGCATCTATGGAAGAAGGAAAGAAGAAAAGAACCAAAGATGAAATTGTAGATATAGATGATCCAGAGACCAAGCGCTTTCCTTATCCTCTTAATGAATTATTAATTTGGGCTTGCCTTATGAAGAGGCAGGTCATGGCCCGCTTTTTATGGCAGCATGGTGAAGAATCAATGGCTAAAGCATTAGTTGCCTGTAAAATCTATCGTTCAATGGCTTATGAGGCAAAGCAGAGTGACCTGGTAGATGATACTTCAGAGGAACTGAAGCAGTATTCCAATGATTTTGGCCAACTGGCAGTTGAATTACTGGAACAGTCCTTCAGACAGGATGAAACGATGGCTATGAAATTACTCACTTATGAACTCAAAAACTGGAGTAATTCAACCTGCCTCAAGTTAGCAGTTTCTTCAAGACTTAGACCTTTTGTAGCTCACACTTGTACACAGATGTTGTTATCTGATATGTGGATGGGACGGCTGAATATGAGAAAAAATTCCTGGTATAAGGTCATATTAAGCATTTTAGTTCCACCTGCCATATTAATGCTAGAGTATAAAACCAAGGCTGAAATGTCCCATATCCCACAATCTCAAGATGCTCATCAAATGACGATGGAGGATAGTGAAAACAATTTTCACAACATAACAGAAGAGATACCCATGGAAGTATTTAAAGAAGTAAAGATTTTGGACAGCAGTGATGGAAAGAATGAAATGGAGATACATATTAAATCAAAAAAGCTTCCAATCACACGAAAATTTTATGCCTTTTATCATGCACCAATTGTAAAGTTCTGGTTTAACACATTGGCATATTTAGGATTTCTGATGCTTTATACATTTGTAGTTCTTGTAAAAATGGAACAGTTACCTTCAGTTCAAGAATGGATTGTTATCGCTTATATTTTTACCTATGCTATTGAAAAAGTCCGTGAGGTCTTCATGTCTGAAGCTGGGAAAATCAGCCAGAAGATTAAAGTATGGTTTAGTGACTACTTCAATGTCAGTGACACAATTGCCATCATTTCTTTCTTTGTTGGATTTGGACTAAGATTTGGAGCAAAATGGAACTATATTAATGCATATGATAATCATGTTTTTGTGGCTGGAAGATTAATTTACTGTCTTAATATAATATTTTGGTATGTGCGTTTGCTAGACTTTCTAGCCGTAAATCAACAGGCAGGACCTTATGTAATGATGATTGGAAAAATGGTGGCCAATATGTTCTACATTGTAGTGATAATGGCTCTTGTATTGCTTAGTTTTGGTGTTCCCAGAAAAGCAATACTTTATCCACATGAAGAACCATCTTGGTCTCTTGCTAAAGATATAGTTTTTCATCCATACTGGATGATTTTTGGTGAAGTTTATGCATATGAAATTGATGTGTGTGCAAATGACTCCACTCTCCCGACAATCTGTGGTCCTGGAACTTGGTTGACTCCATTTCTTCAAGCAGTCTACCTCTTTGTACAGTATATCATTATGGTTAATCTCCTTATCGCATTTTTCAATAATGTATATTTACAAGTGAAGGCAATTTCCAATATTGTATGGAAGTATCAGCGGTATCATTTTATTATGGCTTATCATGAAAAACCAGTCCTGCCTCCTCCTCTTATCATCCTCAGCCATATAGTTTCACTGTTTTGCTGTGTATGCAAAAGAAGAAAGAAAGATAAGACTTCCGATGGGCCAAAACTTTTCTTAACAGAAGAAGATCAAAAGAAACTCCATGATTTTGAAGAGCAGTGTGTTGAGATGTACTTTGATGAGAAAGATGACAAATTCAATTCTGGGAGTGAAGAGAGAATCCGGGTCACTTTTGAAAGAGTGGAGCAGATGAGCATTCAGATTAAAGAAGTTGGAGATCGTGTCAACTACATAAAAAGATCATTACAGTCTTTAGATTCTCAAATTGGTCATCTGCAAGATCTCTCAGCCCTAACAGTAGATACATTGAAAACACTTACAGCCCAGAAAGCTTCAGAAGCTAGTAAAGTGCACAATGAGATCACACGAGAATTGAGTATTTCCAAACACTTGGCTCAGAATCTTATTGATGATGTTCCTGTAAGACCTTTGTGGAAGAAACCTAGTGCTGTAAACACACTGAGTTCCTCTCTTCCTCAAGGTGATCGGGAAAGTAATAATCCTTTTCTTTGTAATATTTTTATGAAAGATGAAAAAGACCCCCAATATAATCTGTTTGGACAAGATTTGCCCGTGATACCCCAGAGAAAAGAATTCAACATTCCAGAGGCTGGTTCCTCCTGTGGTGCCTTATTCCCAAGTGCTGTTTCTCCCCCAGAATTACGACAGAGACGACATGGGGTAGAAATGTTAAAAATATTTAATAAAAATCAAAAATTAGGCAGTTCACCTAATAGTTCACCACATATGTCCTCCCCACCAACCAAATTTTCTGTGAGTACCCCATCCCAGCCAAGTTGCAAAAGTCACTTGGAATCCACAACCAAAGATCAAGAACCCATTTTCTATAAAGCTGCAGAAGGGGATAACATAGAATTTGGAGCATTTGTGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAGAGTCATGA
Peptide sequence:
MRHNHFIKTAQPYRPKMDASMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILMLEYKTKAEMSHIPQSQDAHQMTMEDSENNFHNITEEIPMEVFKEVKILDSSDGKNEMEIHIKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVKMEQLPSVQEWIVIAYIFTYAIEKVREVFMSEAGKISQKIKVWFSDYFNVSDTIAIISFFVGFGLRFGAKWNYINAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEEPSWSLAKDIVFHPYWMIFGEVYAYEIDVCANDSTLPTICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCVCKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFDEKDDKFNSGSEERIRVTFERVEQMSIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDVPVRPLWKKPSAVNTLSSSLPQGDRESNNPFLCNIFMKDEKDPQYNLFGQDLPVIPQRKEFNIPEAGSSCGALFPSAVSPPELRQRRHGVEMLKIFNKNQKLGSSPNSSPHMSSPPTKFSVSTPSQPSCKSHLESTTKDQEPIFYKAAEGDNIEFGAFVEVRSKYLQQHPSVAKES
IRESfinder result
IRESfinder score
0.721960062
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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