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mmu_circTrpm7_1110
Basic information of circular RNA
PanCircBase ID:
mmu_circTrpm7_1110
mm39_Chr. Position ID:
chr2|126672048|126688165|-
Gene name:
Trpm7
Transcript:
ENSMUST00000103224.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
15,14,13,12,11,10,9,8,7
Exon/intron offsets:
0,1068,3559,5327,7206,10615,12727,14171,15945
Genomic length:
16117
Exon/intron sizes in nt:
13,51,41,54,13,51,01,70,00,00,00,000
Flanking exon/introns:
chr2:126671210-126672048|chr2:126688165-126690416
Splice length:
1110
Other database IDs:
circAtlas: mmu-Trpm7_0089
Human Ortholog IDs:
Mature spliced Sequence:
GTGGTTGCTCCTTATCAAACCCTATTGAATCCCTTGAGCAAATTGAATGTTCTGAATAATCTACACTCCCATTTCATCTTGGTGGATGATGGCACTGTTGGAAAGTATGGGGCAGAAGTCAGACTGAGAAGAGAACTTGAAAAAACCATTAATCAGCAAAGAATTCATGCTAGAATTGGGCAAGGAGTTCCTGTGGTGGCTTTGATATTTGAAGGCGGGCCAAATGTCATCCTTACAGTACTGGAGTACCTTCAGGAAAGCCCCCCAGTTCCAGTTGTTGTGTGTGAAGGGACAGGCAGAGCTGCAGATTTACTAGCCTATATCCACAAACAGACAGAGGAAGGAGGAAATCTTCCTGATGCAGCAGAGCCTGATATTATATCAACTATCAAGAAAACATTTAACTTTGGCCAGAGTGAAGCAGTTCATTTATTTCAAACAATGATGGAGTGTATGAAAAAAAAAGAGCTTATCACTGTTTTTCACATTGGATCAGAGGATCATCAAGATATAGATGTGGCCATACTCACTGCACTGCTGAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACACTGGCATGGGACAGAGTTGATATTGCCAAAAATCATGTATTTGTTTATGGACAACAGTGGCTGGTTGGATCCTTGGAACAGGCTATGCTTGATGCTCTTGTAATGGACAGAGTTTCATTTGTAAAACTTCTTATTGAAAACGGAGTAAGCATGCATAAATTCCTTACCATTCCCAGACTGGAAGAACTTTATAACACTAAACAAGGTCCAACCAATCCAATGTTGTTCCATCTCATTCGGGATGTCAAGCAGGGTAATCTCCCCCCGGGGTACAAGATCACTTTAATTGATATAGGACTTGTGATTGAGTATCTCATGGGAGGAACCTACAGATGCACATACACACGAAAACGTTTTCGATTGATATATAATAGTCTTGGTGGAAATAACCGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAGAGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAGCCCAACCCTACAGACCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACTTTTGGCAATAGAGCTGA
55.77
ACAGTGCCATCATCCACCAA
59.59
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACTTTTGGCAATAGAGCTGAT
55.55
ACAGTGCCATCATCCACCAA
59.59
179
PCR template sequence:
GTTGCGAAAGAGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAGCCCAACCCTACAGACCAAAGGTGGTTGCTCCTTATCAAACCCTATTGAATCCCTTGAGCAAATTGAATGTTCTGAATAATCTACACTCCCATTTCATCTTGGTGGATGATGGCACTGTTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-335-5p(1), mmu-miR-217-5p(1), mmu-miR-203-3p(1), mmu-miR-181b-5p(1), mmu-miR-182-5p(1), mmu-miR-541-5p(1), mmu-miR-297a-5p(1), mmu-miR-377-3p(1), mmu-miR-296-3p(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTACAGACCAAAGGTGGTTGCTCCTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTRPM7_1110_ORF_1
0.8751
0.033140884
0.999909106
1779
ORF sequence:
ATGATGGAGTGTATGAAAAAAAAAGAGCTTATCACTGTTTTTCACATTGGATCAGAGGATCATCAAGATATAGATGTGGCCATACTCACTGCACTGCTGAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACACTGGCATGGGACAGAGTTGATATTGCCAAAAATCATGTATTTGTTTATGGACAACAGTGGCTGGTTGGATCCTTGGAACAGGCTATGCTTGATGCTCTTGTAATGGACAGAGTTTCATTTGTAAAACTTCTTATTGAAAACGGAGTAAGCATGCATAAATTCCTTACCATTCCCAGACTGGAAGAACTTTATAACACTAAACAAGGTCCAACCAATCCAATGTTGTTCCATCTCATTCGGGATGTCAAGCAGGGTAATCTCCCCCCGGGGTACAAGATCACTTTAATTGATATAGGACTTGTGATTGAGTATCTCATGGGAGGAACCTACAGATGCACATACACACGAAAACGTTTTCGATTGATATATAATAGTCTTGGTGGAAATAACCGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAGAGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAGCCCAACCCTACAGACCAAAGGTGGTTGCTCCTTATCAAACCCTATTGAATCCCTTGAGCAAATTGAATGTTCTGAATAATCTACACTCCCATTTCATCTTGGTGGATGATGGCACTGTTGGAAAGTATGGGGCAGAAGTCAGACTGAGAAGAGAACTTGAAAAAACCATTAATCAGCAAAGAATTCATGCTAGAATTGGGCAAGGAGTTCCTGTGGTGGCTTTGATATTTGAAGGCGGGCCAAATGTCATCCTTACAGTACTGGAGTACCTTCAGGAAAGCCCCCCAGTTCCAGTTGTTGTGTGTGAAGGGACAGGCAGAGCTGCAGATTTACTAGCCTATATCCACAAACAGACAGAGGAAGGAGGAAATCTTCCTGATGCAGCAGAGCCTGATATTATATCAACTATCAAGAAAACATTTAACTTTGGCCAGAGTGAAGCAGTTCATTTATTTCAAACAATGATGGAGTGTATGAAAAAAAAAGAGCTTATCACTGTTTTTCACATTGGATCAGAGGATCATCAAGATATAGATGTGGCCATACTCACTGCACTGCTGAAAGGTACTAATGCATCTGCATTTGACCAGCTTATCCTTACACTGGCATGGGACAGAGTTGATATTGCCAAAAATCATGTATTTGTTTATGGACAACAGTGGCTGGTTGGATCCTTGGAACAGGCTATGCTTGATGCTCTTGTAATGGACAGAGTTTCATTTGTAAAACTTCTTATTGAAAACGGAGTAAGCATGCATAAATTCCTTACCATTCCCAGACTGGAAGAACTTTATAACACTAAACAAGGTCCAACCAATCCAATGTTGTTCCATCTCATTCGGGATGTCAAGCAGGGTAATCTCCCCCCGGGGTACAAGATCACTTTAATTGATATAGGACTTGTGATTGAGTATCTCATGGGAGGAACCTACAGATGCACATACACACGAAAACGTTTTCGATTGATATATAATAGTCTTGGTGGAAATAACCGGAGGTCAGGTCGAAATACCTCCAGCAGCACCCCTCAGTTGCGAAAGAGTCATGAAACTTTTGGCAATAGAGCTGATAAAAAGGAAAAAATGAGACACAATCATTTCATTAAAACAGCCCAACCCTACAGACCAAAG
Peptide sequence:
MMECMKKKELITVFHIGSEDHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVSFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLIRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHETFGNRADKKEKMRHNHFIKTAQPYRPKVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQSEAVHLFQTMMECMKKKELITVFHIGSEDHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVSFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLIRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHETFGNRADKKEKMRHNHFIKTAQPYRPK
IRESfinder result
IRESfinder score
0.737467965
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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