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mmu_circTrip10_452
Basic information of circular RNA
PanCircBase ID:
mmu_circTrip10_452
mm39_Chr. Position ID:
chr17|57562017|57564344|+
Gene name:
Trip10
Transcript:
ENSMUST00000224947.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
8,9,10
Exon/intron offsets:
0,255,2217
Genomic length:
2327
Exon/intron sizes in nt:
14,71,95,110
Flanking exon/introns:
chr17:57561388-57562017|chr17:57564344-57568289
Splice length:
452
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AAGCTGCAGGACATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCTTATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAATGCTTGGAGGGCATGAAGGTGGCCGCCGAGTCTGTGGATGCTAAGAACGACTCACAGGTCCTCATCGAATTACACAAGTCCGGGTTTGCCCGCCCAGGGGACTTGGAATTTGAAGACTTTAGCCAAGTTATCAACCGAGTGCCTTCGGACAGCAGCCTGGGCACCCCGGATGGCAGGCCTGAGCTCCGAGCAGCCTCCAGCCGTAGCCGCGCCAAGCGTTGGCCTTTCGGGAAAAAGAACAAGACCGTGGCTACCGAAGATTTCAGTCACTTGCCCCCGGAGCAGCAGAGAAAGCGACTTCAGCAACAGCTGGAAGAGCGGAACCGAGAGTTGCAGAAGGAGGAGGACCAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGCAGCAGAGAAAGCGACTT
59.97
CCTGCAGTTCAGCCTCTGAT
59.75
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AAAGCGACTTCAGCAACAGC
59.69
CCTGCAGTTCAGCCTCTGAT
59.75
142
PCR template sequence:
CCGAAGATTTCAGTCACTTGCCCCCGGAGCAGCAGAGAAAGCGACTTCAGCAACAGCTGGAAGAGCGGAACCGAGAGTTGCAGAAGGAGGAGGACCAGAGAAGCTGCAGGACATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCTTATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAAT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-124-3p(1), mmu-miR-205-5p(1), mmu-miR-666-5p(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
AGGAGGACCAGAGAAGCTGCAGGACA
siRNA sets
TGTCCTGCAGCTTCTCTGG, CAGCTTCTCTGGTCCTCCT, CTGCAGCTTCTCTGGTCCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTRIP10_452_ORF_1
0.8274
0.356115043
0.850109269
555
ORF sequence:
ATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCTTATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAATGCTTGGAGGGCATGAAGGTGGCCGCCGAGTCTGTGGATGCTAAGAACGACTCACAGGTCCTCATCGAATTACACAAGTCCGGGTTTGCCCGCCCAGGGGACTTGGAATTTGAAGACTTTAGCCAAGTTATCAACCGAGTGCCTTCGGACAGCAGCCTGGGCACCCCGGATGGCAGGCCTGAGCTCCGAGCAGCCTCCAGCCGTAGCCGCGCCAAGCGTTGGCCTTTCGGGAAAAAGAACAAGACCGTGGCTACCGAAGATTTCAGTCACTTGCCCCCGGAGCAGCAGAGAAAGCGACTTCAGCAACAGCTGGAAGAGCGGAACCGAGAGTTGCAGAAGGAGGAGGACCAGAGAAGCTGCAGGACATGGACGAACGCCGGGCCACCCGCCTGGGGGCCGGGTATGGGCTCTTATCAGAGGCTGAACTGCAGGTGGTTCCCATTATTGGCAAATGCTTGGAGGGCATGA
Peptide sequence:
MDERRATRLGAGYGLLSEAELQVVPIIGKCLEGMKVAAESVDAKNDSQVLIELHKSGFARPGDLEFEDFSQVINRVPSDSSLGTPDGRPELRAASSRSRAKRWPFGKKNKTVATEDFSHLPPEQQRKRLQQQLEERNRELQKEEDQRSCRTWTNAGPPAWGPGMGSYQRLNCRWFPLLANAWRA
IRESfinder result
IRESfinder score
0.325302465
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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