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mmu_circTpr_745
Basic information of circular RNA
PanCircBase ID:
mmu_circTpr_745
mm39_Chr. Position ID:
chr1|150289394|150293267|+
Gene name:
Tpr
Transcript:
ENSMUST00000124973.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
16,17,18,19,20
Exon/intron offsets:
0,772,1022,2425,3738
Genomic length:
3873
Exon/intron sizes in nt:
16,41,34,14,91,63,135
Flanking exon/introns:
chr1:150288868-150289394|chr1:150293267-150293698
Splice length:
745
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GATTGCTGAACTTCAGCATAAACTTGAGAACTCTCTCGCTGAACTGGAACAGCTCCGTGAGTCACGACAACATCAGATGCAGCTTGTTGATTCCATAGTGCGTCAGCGTGACATGTACCGAATTTTATTGTCACAAACAACAGGAATGGCCATTCCCTTACAAGCTTCAAGTTTAGATGATATTTCTCTTCTCTCAACTCCAAAACGTTCAAGTACATCACAGACTGTTTCCACTCCTGCTCCGGAACCCGTCATTGACTCCACAGAGGCTATAGAAGCAAAGGCTGCCCTTAAACAGTTGCAGGAAATCTTTGAGAACTATAAAAAAGAAAAAATAGACAGTGAGAAACTTCAAAATGAGCAGCTAGAGAAACTTCAAGAGCAAGTCACAGACCTGCGCTCACAAAACACCAAAATTTCTACTCAGCTAGATTTTGCTTCTAAACGTTATGAAATGCTGCAAGATAATGTAGAAGGATATCGTCGAGAAATAACATCTCTACAAGAGAGAAATCAGAAACTCACTGCAACTACTCAGAAGCAGGAACAGATCATCAACACAATGACGCAAGACTTGAGAGGAGCCAATGAAAAGCTAGCAGTTGCAGAAGTAAGAGCAGAAAATTTAAAGAAGGAAAAAGAAATGCTTAAATTGTCTGAAGTCCGTCTTTCTCAGCAAAGAGAGTCTTTGTTAGCTGAACAGAGGGGGCAGAACTTGTTGCTGACTAATCTACAGACAATCCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAAGTCCGTCTTTCTCAGCA
59.31
GCTGTTCCAGTTCAGCGAGA
60.32
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTTAGCTGAACAGAGGGGGC
60.39
AAGCTGCATCTGATGTTGTCG
59.26
140
PCR template sequence:
GCTTAAATTGTCTGAAGTCCGTCTTTCTCAGCAAAGAGAGTCTTTGTTAGCTGAACAGAGGGGGCAGAACTTGTTGCTGACTAATCTACAGACAATCCAGGATTGCTGAACTTCAGCATAAACTTGAGAACTCTCTCGCTGAACTGGAACAGCTCCGTGAGTCACGACAACATCAGATGCAGCTTGTTGATTCCATAGTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-103-3p(1), mmu-miR-221-3p(1), mmu-miR-107-3p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACAGACAATCCAGGATTGCTGAACTT
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTPR_745_ORF_1
1.0438
0.103196359
0.878442291
678
ORF sequence:
ATGCAGCTTGTTGATTCCATAGTGCGTCAGCGTGACATGTACCGAATTTTATTGTCACAAACAACAGGAATGGCCATTCCCTTACAAGCTTCAAGTTTAGATGATATTTCTCTTCTCTCAACTCCAAAACGTTCAAGTACATCACAGACTGTTTCCACTCCTGCTCCGGAACCCGTCATTGACTCCACAGAGGCTATAGAAGCAAAGGCTGCCCTTAAACAGTTGCAGGAAATCTTTGAGAACTATAAAAAAGAAAAAATAGACAGTGAGAAACTTCAAAATGAGCAGCTAGAGAAACTTCAAGAGCAAGTCACAGACCTGCGCTCACAAAACACCAAAATTTCTACTCAGCTAGATTTTGCTTCTAAACGTTATGAAATGCTGCAAGATAATGTAGAAGGATATCGTCGAGAAATAACATCTCTACAAGAGAGAAATCAGAAACTCACTGCAACTACTCAGAAGCAGGAACAGATCATCAACACAATGACGCAAGACTTGAGAGGAGCCAATGAAAAGCTAGCAGTTGCAGAAGTAAGAGCAGAAAATTTAAAGAAGGAAAAAGAAATGCTTAAATTGTCTGAAGTCCGTCTTTCTCAGCAAAGAGAGTCTTTGTTAGCTGAACAGAGGGGGCAGAACTTGTTGCTGACTAATCTACAGACAATCCAGGATTGCTGA
Peptide sequence:
MQLVDSIVRQRDMYRILLSQTTGMAIPLQASSLDDISLLSTPKRSSTSQTVSTPAPEPVIDSTEAIEAKAALKQLQEIFENYKKEKIDSEKLQNEQLEKLQEQVTDLRSQNTKISTQLDFASKRYEMLQDNVEGYRREITSLQERNQKLTATTQKQEQIINTMTQDLRGANEKLAVAEVRAENLKKEKEMLKLSEVRLSQQRESLLAEQRGQNLLLTNLQTIQDC
IRESfinder result
IRESfinder score
0.710104133
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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