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mmu_circTpr_1497
Basic information of circular RNA
PanCircBase ID:
mmu_circTpr_1497
mm39_Chr. Position ID:
chr1|150306912|150315949|+
Gene name:
Tpr
Transcript:
ENSMUST00000124973.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
34,35,36,37,38,39,40,41,42
Exon/intron offsets:
0,1249,1657,2516,4481,5556,6241,7920,8905
Genomic length:
9037
Exon/intron sizes in nt:
18,72,06,62,20,92,73,10,00,00,00,000
Flanking exon/introns:
chr1:150306455-150306912|chr1:150315949-150316189
Splice length:
1497
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ACATTATCTGAAAAAGAGACAGAAGCAAGAAGTCTCCAGGAGCAGACGGTGCAGCTTCAGTCTGAACTGTCACGCCTTCGTCAGGACCTCCAGGATAAAACCACAGAGGAGCAGCTCCGACAGCAAATGAATGAAAAGACTTGGAAGACACTTGCACTAGCCAAATCAAAAATTACACATCTATCTGGTGTGAAAGATCAGCTGACTAAAGAAATTGAAGAACTTAAACAAAGGAACGGAGCTCTAGATCAGCAGAAAGATGAGCTTGATGTCCGAATGACTGCTCTTAAGTCCCAGTATGAAGGTCGGATTAGTCGCTTAGAGAGAGAGCTCAGAGAGCATCAAGAGCGACACCTTGAGCAGAGGGATGAGCCTCAAGAACCCACTAATAAGGCTCCAGAACAGCAAAGACAAATCACACTGAAAACCACTCCAGCTTCTGGTGAAAGAGGAATTGCCAGCACATCAGACCCACCAACAGCCAATATCAAGCCAACTCCTGTTGTTTCTACTCCAAGTAAAGTGACAGCTGCAGCTATGGCTGGGAATAAGTCGACACCCAGGGCAAGTATCCGCCCAATGGTTACACCTGCAACGGTCACAAATCCGACCACTACCCCAACAGCAACCGTAATGCCTACTACACAGGTGGAGTCACAGGAAGCTATGCAGTCAGAAGGGCCTGTGGAACATGTTCCAGTATTTGGAAACGCAAGTGGATCTGTTCGTTCTACTAGTCCTAATGTTCAGCCCTCCATTTCTCAGCCCATCCTAACTGTACAGCAGCAAACACAAGCTACAGCTTTTGTACAACCCACTCAACAGAGCCACCCGCAGATTGAGCCTACAAATCAAGAATTGTCCCCAAACATAGTTGAGGTAGTACAAAGTTCCCCAGTGGAGCGTCCATCTACTTCCACAGCAGTATTTGGCACTGTTTCAGCTACCCCAAGTTCCTCCTTGCCAAAGCGCACTCGGGAGGAGGAAGAGGACAGCACCATGGAAGCAGGAGACCAAGTCTCTGAGGACACAGTGGAAATGCCTCTCCCGAAGAAGCTGAAGATGGTCACACCCGTTGGAACCGAGGAAGAAGTTATGGCAGAAGAAAGTACTGATGGAGAGGCAGAAACTCAAGCCTATAATCAGGACTCTCAAGATTCCATTGGAGAAGGAGTTACCCAGGGAGATTATACACCAATGGAAGACAGTGAAGAAACATCACAATCTCTACAAATCGATCTTGGACCACTTCAGTCAGATCAACAGACTACTTCCTCTCAAGATGGTCAAGGCAAAGGAGATGATGTAATTGTAATTGACAGTGATGATGAAGATGATGATGAAGAAAATGATGGCGAACATGAAGATTATGAAGAAGACGAAGATGATGATGATGATGAAGAAGATGACACGGGGATGGGAGATGAGGGTGAAGATAGTAATGAAGGAACTGGTAGTGCAGATGGTAATGATGGATATGAAGCTGATGATGCTGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGGATGGGAGATGAGGGTGA
60.4
ATCCTGGAGGTCCTGACGAA
59.96
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAAGATGACACGGGGATGGG
60.18
CGAAGGCGTGACAGTTCAGA
60.32
176
PCR template sequence:
TGAAGAAGATGACACGGGGATGGGAGATGAGGGTGAAGATAGTAATGAAGGAACTGGTAGTGCAGATGGTAATGATGGATATGAAGCTGATGATGCTGAGACATTATCTGAAAAAGAGACAGAAGCAAGAAGTCTCCAGGAGCAGACGGTGCAGCTTCAGTCTGAACTGTCACGCCTTCGTCAGGACCTCCAGGATAAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
15
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-200c-3p(1), mmu-miR-326-3p(1), mmu-miR-181b-5p(1), mmu-miR-182-5p(1), mmu-miR-301b-3p(1), mmu-miR-758-3p(1), mmu-miR-541-5p(1), mmu-miR-301a-3p(1), mmu-miR-335-3p(1), mmu-miR-669d-5p(1), mmu-miR-1904(1), mmu-miR-181d-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGATGATGCTGAGACATTATCTGAAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTPR_1497_ORF_1
1.1486
0.251768919
0.999999991
2868
ORF sequence:
ATGAATGAAAAGACTTGGAAGACACTTGCACTAGCCAAATCAAAAATTACACATCTATCTGGTGTGAAAGATCAGCTGACTAAAGAAATTGAAGAACTTAAACAAAGGAACGGAGCTCTAGATCAGCAGAAAGATGAGCTTGATGTCCGAATGACTGCTCTTAAGTCCCAGTATGAAGGTCGGATTAGTCGCTTAGAGAGAGAGCTCAGAGAGCATCAAGAGCGACACCTTGAGCAGAGGGATGAGCCTCAAGAACCCACTAATAAGGCTCCAGAACAGCAAAGACAAATCACACTGAAAACCACTCCAGCTTCTGGTGAAAGAGGAATTGCCAGCACATCAGACCCACCAACAGCCAATATCAAGCCAACTCCTGTTGTTTCTACTCCAAGTAAAGTGACAGCTGCAGCTATGGCTGGGAATAAGTCGACACCCAGGGCAAGTATCCGCCCAATGGTTACACCTGCAACGGTCACAAATCCGACCACTACCCCAACAGCAACCGTAATGCCTACTACACAGGTGGAGTCACAGGAAGCTATGCAGTCAGAAGGGCCTGTGGAACATGTTCCAGTATTTGGAAACGCAAGTGGATCTGTTCGTTCTACTAGTCCTAATGTTCAGCCCTCCATTTCTCAGCCCATCCTAACTGTACAGCAGCAAACACAAGCTACAGCTTTTGTACAACCCACTCAACAGAGCCACCCGCAGATTGAGCCTACAAATCAAGAATTGTCCCCAAACATAGTTGAGGTAGTACAAAGTTCCCCAGTGGAGCGTCCATCTACTTCCACAGCAGTATTTGGCACTGTTTCAGCTACCCCAAGTTCCTCCTTGCCAAAGCGCACTCGGGAGGAGGAAGAGGACAGCACCATGGAAGCAGGAGACCAAGTCTCTGAGGACACAGTGGAAATGCCTCTCCCGAAGAAGCTGAAGATGGTCACACCCGTTGGAACCGAGGAAGAAGTTATGGCAGAAGAAAGTACTGATGGAGAGGCAGAAACTCAAGCCTATAATCAGGACTCTCAAGATTCCATTGGAGAAGGAGTTACCCAGGGAGATTATACACCAATGGAAGACAGTGAAGAAACATCACAATCTCTACAAATCGATCTTGGACCACTTCAGTCAGATCAACAGACTACTTCCTCTCAAGATGGTCAAGGCAAAGGAGATGATGTAATTGTAATTGACAGTGATGATGAAGATGATGATGAAGAAAATGATGGCGAACATGAAGATTATGAAGAAGACGAAGATGATGATGATGATGAAGAAGATGACACGGGGATGGGAGATGAGGGTGAAGATAGTAATGAAGGAACTGGTAGTGCAGATGGTAATGATGGATATGAAGCTGATGATGCTGAGACATTATCTGAAAAAGAGACAGAAGCAAGAAGTCTCCAGGAGCAGACGGTGCAGCTTCAGTCTGAACTGTCACGCCTTCGTCAGGACCTCCAGGATAAAACCACAGAGGAGCAGCTCCGACAGCAAATGAATGAAAAGACTTGGAAGACACTTGCACTAGCCAAATCAAAAATTACACATCTATCTGGTGTGAAAGATCAGCTGACTAAAGAAATTGAAGAACTTAAACAAAGGAACGGAGCTCTAGATCAGCAGAAAGATGAGCTTGATGTCCGAATGACTGCTCTTAAGTCCCAGTATGAAGGTCGGATTAGTCGCTTAGAGAGAGAGCTCAGAGAGCATCAAGAGCGACACCTTGAGCAGAGGGATGAGCCTCAAGAACCCACTAATAAGGCTCCAGAACAGCAAAGACAAATCACACTGAAAACCACTCCAGCTTCTGGTGAAAGAGGAATTGCCAGCACATCAGACCCACCAACAGCCAATATCAAGCCAACTCCTGTTGTTTCTACTCCAAGTAAAGTGACAGCTGCAGCTATGGCTGGGAATAAGTCGACACCCAGGGCAAGTATCCGCCCAATGGTTACACCTGCAACGGTCACAAATCCGACCACTACCCCAACAGCAACCGTAATGCCTACTACACAGGTGGAGTCACAGGAAGCTATGCAGTCAGAAGGGCCTGTGGAACATGTTCCAGTATTTGGAAACGCAAGTGGATCTGTTCGTTCTACTAGTCCTAATGTTCAGCCCTCCATTTCTCAGCCCATCCTAACTGTACAGCAGCAAACACAAGCTACAGCTTTTGTACAACCCACTCAACAGAGCCACCCGCAGATTGAGCCTACAAATCAAGAATTGTCCCCAAACATAGTTGAGGTAGTACAAAGTTCCCCAGTGGAGCGTCCATCTACTTCCACAGCAGTATTTGGCACTGTTTCAGCTACCCCAAGTTCCTCCTTGCCAAAGCGCACTCGGGAGGAGGAAGAGGACAGCACCATGGAAGCAGGAGACCAAGTCTCTGAGGACACAGTGGAAATGCCTCTCCCGAAGAAGCTGAAGATGGTCACACCCGTTGGAACCGAGGAAGAAGTTATGGCAGAAGAAAGTACTGATGGAGAGGCAGAAACTCAAGCCTATAATCAGGACTCTCAAGATTCCATTGGAGAAGGAGTTACCCAGGGAGATTATACACCAATGGAAGACAGTGAAGAAACATCACAATCTCTACAAATCGATCTTGGACCACTTCAGTCAGATCAACAGACTACTTCCTCTCAAGATGGTCAAGGCAAAGGAGATGATGTAATTGTAATTGACAGTGATGATGAAGATGATGATGAAGAAAATGATGGCGAACATGAAGATTATGAAGAAGACGAAGATGATGATGATGATGAAGAAGATGACACGGGGATGGGAGATGAGGGTGAAGATAGTAATGAAGGAACTGGTAGTGCAGATGGTAATGATGGATATGAAGCTGATGATGCTGAG
Peptide sequence:
MNEKTWKTLALAKSKITHLSGVKDQLTKEIEELKQRNGALDQQKDELDVRMTALKSQYEGRISRLERELREHQERHLEQRDEPQEPTNKAPEQQRQITLKTTPASGERGIASTSDPPTANIKPTPVVSTPSKVTAAAMAGNKSTPRASIRPMVTPATVTNPTTTPTATVMPTTQVESQEAMQSEGPVEHVPVFGNASGSVRSTSPNVQPSISQPILTVQQQTQATAFVQPTQQSHPQIEPTNQELSPNIVEVVQSSPVERPSTSTAVFGTVSATPSSSLPKRTREEEEDSTMEAGDQVSEDTVEMPLPKKLKMVTPVGTEEEVMAEESTDGEAETQAYNQDSQDSIGEGVTQGDYTPMEDSEETSQSLQIDLGPLQSDQQTTSSQDGQGKGDDVIVIDSDDEDDDEENDGEHEDYEEDEDDDDDEEDDTGMGDEGEDSNEGTGSADGNDGYEADDAETLSEKETEARSLQEQTVQLQSELSRLRQDLQDKTTEEQLRQQMNEKTWKTLALAKSKITHLSGVKDQLTKEIEELKQRNGALDQQKDELDVRMTALKSQYEGRISRLERELREHQERHLEQRDEPQEPTNKAPEQQRQITLKTTPASGERGIASTSDPPTANIKPTPVVSTPSKVTAAAMAGNKSTPRASIRPMVTPATVTNPTTTPTATVMPTTQVESQEAMQSEGPVEHVPVFGNASGSVRSTSPNVQPSISQPILTVQQQTQATAFVQPTQQSHPQIEPTNQELSPNIVEVVQSSPVERPSTSTAVFGTVSATPSSSLPKRTREEEEDSTMEAGDQVSEDTVEMPLPKKLKMVTPVGTEEEVMAEESTDGEAETQAYNQDSQDSIGEGVTQGDYTPMEDSEETSQSLQIDLGPLQSDQQTTSSQDGQGKGDDVIVIDSDDEDDDEENDGEHEDYEEDEDDDDDEEDDTGMGDEGEDSNEGTGSADGNDGYEADDAE
IRESfinder result
IRESfinder score
0.367215009
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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