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mmu_circTnr_2125
Basic information of circular RNA
PanCircBase ID:
mmu_circTnr_2125
mm39_Chr. Position ID:
chr1|159565080|159701863|+
Gene name:
Tnr
Transcript:
ENSMUST00000192069.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
8
Exon/intron indexes:
2,3,4,5,6,7,8,9
Exon/intron offsets:
0,112475,114446,120666,123699,126127,130505,136597
Genomic length:
136783
Exon/intron sizes in nt:
1,01,56,04,77,26,41,16,00,00,00,000
Flanking exon/introns:
chr1:159351785-159565080|chr1:159701863-159712340
Splice length:
2125
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTGTCTGCATCAACTCATTGCTCAGAGGTCAGATCTCATCTGGAGTTAGAGTCACAGAGCACGTGGTCAAGAGAAACCATCAGAGAGGACGACATCACGAGATTCCTATAACTCCATGCTGGCTACCACTGAGAGCTCTTGAACCAAAGTCTGGCTGAGAAGATGGGGATCGATGGGGAAACAGTAGTCTTGAAGAACATGCTCATTGGTGTAAACCTGATCCTGTTGGGATCCATGCTCAAGCCTTCTGAGTGTCGGCTGGAGGTGACTACAGAAAGGGCTCAGAGACAAACTGTGGAGGAGGAAGGAGGGGCTTCCAGCTACAATACTTCCAGCAAGGAACAGCCTATGGTCTTCAACCATGTGTATAACATCAACGTGCCACTTGAAAGCCTCTGCTCCTCGGGGCTGGAGGCCTCAGCTGAGCAGGACATGAGTGCTGAAGATGATACTCTGGCCGAATACATAGGCCAAACCTCAGACCATGAAAGCCAGGTTACCTTCACCCACAAGATCAACCTCCCCAAAAAAGCCTGCCCATGTGCAAGCTCTTCCCAGGTACTGCAGGAACTGCTGAGCCGGATCGAGATGCTGGAGAGGGAGGTGTCCTTGCTGCGAGACCAGTGCAATACCAACTGCTGTCAGGAAAGCGCTGCCACAGGACAACTGGACTATGTCCCTCACTGCAGCGGCCATGGCAACTTTAGCTTCGAGTCCTGTGGCTGCATCTGCAATGAAGGCTGGTTTGGCAAGAACTGCTCAGAGCCCTACTGCCCACTGGGCTGCTCCAGTCGGGGTGTGTGTGTCGATGGCCAGTGCATTTGTGACAGTGAATACAGCGGAGATGACTGTTCAGAGCTCCGGTGCCCAACAGACTGCAGTTCCCGAGGGCTCTGTGTGGATGGGGAATGTGTCTGTGAAGAGCCCTACACAGGCGAGGACTGCAGGGAGCTGCGCTGCCCTGGGGACTGTTCAGGGAAGGGGCAATGTGCCAATGGTACCTGCCTGTGCCAAGAGGGCTATGCTGGTGAGGACTGCAGCCAGCGACGGTGTCTGAATGCCTGCAGTGGGCGAGGTCACTGCCAGGAGGGGCTCTGCATCTGTGAGGAAGGCTACCAGGGCCCTGACTGCTCAGCAGTTGCCCCTCCAGAGGACTTGCGAGTGGCTGGTATCAGCGACAGGTCCATTGAGCTGGAATGGGACGGGCCGATGGCAGTGACGGAATATGTGATCTCTTACCAGCCGACGGCCCTGGGGGGCCTTCAGCTCCAGCAGCGGGTGCCTGGAGATTGGAGTGGTGTCACCATCATGGAGCTGGAGCCAGGTCTCACCTACAACATCAGCGTCTACGCTGTCATTAGCAACATCCTCAGCCTTCCCATCACTGCCAAGGTGGCCACTCATCTCTCCACTCCTCAAGGGCTACAGTTCAAGACGATCACAGAGACCACCGTGGAGGTGCAGTGGGAGCCCTTCTCTTTCTCCTTCGACGGGTGGGAGATCAGCTTCATTCCAAAGAACAATGAAGGAGGGGTGATTGCTCAGCTCCCCAGCGATGTGACGTCCTTTAACCAGACAGGACTGAAACCTGGGGAGGAGTACATTGTGAATGTTGTGGCTCTAAAGGAACAAGCCCGGAGCCCTCCTACCTCTGCCAGCGTCTCCACTGTCATTGACGGGCCCACACAGATCCTGGTTCGAGATGTCTCTGATACTGTGGCTTTTGTGGAATGGACCCCACCTCGAGCCAAAGTTGATTTCATTCTTTTAAAATATGGCTTGGTGGGTGGCGAAGGCGGGAAGACGACCTTCCGGCTGCAGCCTCCCTTAAGCCAGTACTCCGTGCAAGCCCTTAGACCTGGCTCGCGCTACGAGGTGTCCATCAGCGCAGTCCGGGGGACCAATGAGAGTGAAGCCTCAAGCACCCAATTTACAACAGAGATTGATGCTCCCAAGAATTTGCGAGTGGGTTCCCGCACAGCAACTAGCCTTGACCTGGAATGGGATAACAGCGAGGCCGAAGCTCAGGAGTACAAGGTTGTGTACAGCACCTTAGCTGGTGAACAGTACCACGAAGTGCTGGTACCCAAGGGCATTGGCCCAACTACCAAGACTACCCTCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACGAAGTGCTGGTACCCAAG
59.97
CGTGATGTCGTCCTCTCTGA
58.91
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACGAAGTGCTGGTACCCAAG
59.97
TCGTGATGTCGTCCTCTCTG
58.91
154
PCR template sequence:
CAGGAGTACAAGGTTGTGTACAGCACCTTAGCTGGTGAACAGTACCACGAAGTGCTGGTACCCAAGGGCATTGGCCCAACTACCAAGACTACCCTCACAGGTGTCTGCATCAACTCATTGCTCAGAGGTCAGATCTCATCTGGAGTTAGAGTCACAGAGCACGTGGTCAAGAGAAACCATCAGAGAGGACGACATCACGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
27
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(3), mmu-miR-338-3p(3), mmu-miR-96-5p(2), mmu-miR-9-5p(1), mmu-let-7a-5p(1), mmu-miR-181a-5p(1), mmu-let-7e-5p(1), mmu-let-7b-5p(1), mmu-miR-34a-5p(1), mmu-miR-92a-3p(1), mmu-miR-205-5p(1), mmu-let-7c-5p(1), mmu-miR-298-5p(1), mmu-miR-132-3p(1), mmu-miR-326-3p(1), mmu-miR-23a-3p(1), mmu-miR-25-3p(1), mmu-miR-138-5p(1), mmu-let-7d-5p(1), mmu-miR-24-3p(1), mmu-miR-149-5p(1), mmu-miR-363-3p(1), mmu-miR-382-3p(1), mmu-miR-212-3p(1), mmu-miR-34b-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-193b-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ACTACCCTCACAGGTGTCTGCATCAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTNR_2125_ORF_1
0.8693
0.326117029
0.999997535
2121
ORF sequence:
ATGGGGATCGATGGGGAAACAGTAGTCTTGAAGAACATGCTCATTGGTGTAAACCTGATCCTGTTGGGATCCATGCTCAAGCCTTCTGAGTGTCGGCTGGAGGTGACTACAGAAAGGGCTCAGAGACAAACTGTGGAGGAGGAAGGAGGGGCTTCCAGCTACAATACTTCCAGCAAGGAACAGCCTATGGTCTTCAACCATGTGTATAACATCAACGTGCCACTTGAAAGCCTCTGCTCCTCGGGGCTGGAGGCCTCAGCTGAGCAGGACATGAGTGCTGAAGATGATACTCTGGCCGAATACATAGGCCAAACCTCAGACCATGAAAGCCAGGTTACCTTCACCCACAAGATCAACCTCCCCAAAAAAGCCTGCCCATGTGCAAGCTCTTCCCAGGTACTGCAGGAACTGCTGAGCCGGATCGAGATGCTGGAGAGGGAGGTGTCCTTGCTGCGAGACCAGTGCAATACCAACTGCTGTCAGGAAAGCGCTGCCACAGGACAACTGGACTATGTCCCTCACTGCAGCGGCCATGGCAACTTTAGCTTCGAGTCCTGTGGCTGCATCTGCAATGAAGGCTGGTTTGGCAAGAACTGCTCAGAGCCCTACTGCCCACTGGGCTGCTCCAGTCGGGGTGTGTGTGTCGATGGCCAGTGCATTTGTGACAGTGAATACAGCGGAGATGACTGTTCAGAGCTCCGGTGCCCAACAGACTGCAGTTCCCGAGGGCTCTGTGTGGATGGGGAATGTGTCTGTGAAGAGCCCTACACAGGCGAGGACTGCAGGGAGCTGCGCTGCCCTGGGGACTGTTCAGGGAAGGGGCAATGTGCCAATGGTACCTGCCTGTGCCAAGAGGGCTATGCTGGTGAGGACTGCAGCCAGCGACGGTGTCTGAATGCCTGCAGTGGGCGAGGTCACTGCCAGGAGGGGCTCTGCATCTGTGAGGAAGGCTACCAGGGCCCTGACTGCTCAGCAGTTGCCCCTCCAGAGGACTTGCGAGTGGCTGGTATCAGCGACAGGTCCATTGAGCTGGAATGGGACGGGCCGATGGCAGTGACGGAATATGTGATCTCTTACCAGCCGACGGCCCTGGGGGGCCTTCAGCTCCAGCAGCGGGTGCCTGGAGATTGGAGTGGTGTCACCATCATGGAGCTGGAGCCAGGTCTCACCTACAACATCAGCGTCTACGCTGTCATTAGCAACATCCTCAGCCTTCCCATCACTGCCAAGGTGGCCACTCATCTCTCCACTCCTCAAGGGCTACAGTTCAAGACGATCACAGAGACCACCGTGGAGGTGCAGTGGGAGCCCTTCTCTTTCTCCTTCGACGGGTGGGAGATCAGCTTCATTCCAAAGAACAATGAAGGAGGGGTGATTGCTCAGCTCCCCAGCGATGTGACGTCCTTTAACCAGACAGGACTGAAACCTGGGGAGGAGTACATTGTGAATGTTGTGGCTCTAAAGGAACAAGCCCGGAGCCCTCCTACCTCTGCCAGCGTCTCCACTGTCATTGACGGGCCCACACAGATCCTGGTTCGAGATGTCTCTGATACTGTGGCTTTTGTGGAATGGACCCCACCTCGAGCCAAAGTTGATTTCATTCTTTTAAAATATGGCTTGGTGGGTGGCGAAGGCGGGAAGACGACCTTCCGGCTGCAGCCTCCCTTAAGCCAGTACTCCGTGCAAGCCCTTAGACCTGGCTCGCGCTACGAGGTGTCCATCAGCGCAGTCCGGGGGACCAATGAGAGTGAAGCCTCAAGCACCCAATTTACAACAGAGATTGATGCTCCCAAGAATTTGCGAGTGGGTTCCCGCACAGCAACTAGCCTTGACCTGGAATGGGATAACAGCGAGGCCGAAGCTCAGGAGTACAAGGTTGTGTACAGCACCTTAGCTGGTGAACAGTACCACGAAGTGCTGGTACCCAAGGGCATTGGCCCAACTACCAAGACTACCCTCACAGGTGTCTGCATCAACTCATTGCTCAGAGGTCAGATCTCATCTGGAGTTAGAGTCACAGAGCACGTGGTCAAGAGAAACCATCAGAGAGGACGACATCACGAGATTCCTATAACTCCATGCTGGCTACCACTGAGAGCTCTTGAACCAAAGTCTGGCTGA
Peptide sequence:
MGIDGETVVLKNMLIGVNLILLGSMLKPSECRLEVTTERAQRQTVEEEGGASSYNTSSKEQPMVFNHVYNINVPLESLCSSGLEASAEQDMSAEDDTLAEYIGQTSDHESQVTFTHKINLPKKACPCASSSQVLQELLSRIEMLEREVSLLRDQCNTNCCQESAATGQLDYVPHCSGHGNFSFESCGCICNEGWFGKNCSEPYCPLGCSSRGVCVDGQCICDSEYSGDDCSELRCPTDCSSRGLCVDGECVCEEPYTGEDCRELRCPGDCSGKGQCANGTCLCQEGYAGEDCSQRRCLNACSGRGHCQEGLCICEEGYQGPDCSAVAPPEDLRVAGISDRSIELEWDGPMAVTEYVISYQPTALGGLQLQQRVPGDWSGVTIMELEPGLTYNISVYAVISNILSLPITAKVATHLSTPQGLQFKTITETTVEVQWEPFSFSFDGWEISFIPKNNEGGVIAQLPSDVTSFNQTGLKPGEEYIVNVVALKEQARSPPTSASVSTVIDGPTQILVRDVSDTVAFVEWTPPRAKVDFILLKYGLVGGEGGKTTFRLQPPLSQYSVQALRPGSRYEVSISAVRGTNESEASSTQFTTEIDAPKNLRVGSRTATSLDLEWDNSEAEAQEYKVVYSTLAGEQYHEVLVPKGIGPTTKTTLTGVCINSLLRGQISSGVRVTEHVVKRNHQRGRHHEIPITPCWLPLRALEPKSG
IRESfinder result
IRESfinder score
0.45904888
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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