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mmu_circTmprss2_733
Basic information of circular RNA
PanCircBase ID:
mmu_circTmprss2_733
mm39_Chr. Position ID:
chr16|97377415|97400522|-
Gene name:
Tmprss2
Transcript:
ENSMUST00000000395.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,2314,14604,15208,20524,23039
Genomic length:
23107
Exon/intron sizes in nt:
111,127,120,87,220,68
Flanking exon/introns:
chr16:97373248-97377415|chr16:97400522-97412218
Splice length:
733
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GTCACCTGGAGGAAGCCCCATACTGACCTCCTCATGCTGCTGACACAGGCAGGATGGCATTGAACTCAGGGTCACCTCCAGGAATCGGACCTTGCTATGAGAACCACGGGTATCAGTCTGAGCACATCTGTCCTCCGAGACCACCAGTGGCTCCCAATGGCTACAACTTGTATCCAGCCCAGTACTACCCATCTCCAGTGCCTCAGTATGCTCCGAGGATTACAACGCAAGCCTCAACATCTGTCATCCACACACATCCCAAGTCCTCAGGAGCACTGTGCACCTCAAAGTCTAAGAAATCGCTGTGTTTAGCCCTCGCCCTGGGCACTGTCCTCACGGGAGCTGCTGTGGCTGCTGTCTTGCTTTGGAGGTTCTGGGACAGCAACTGTTCTACGTCTGAGATGGAGTGTGGGTCTTCAGGCACATGCATCAGCTCTTCTCTCTGGTGTGACGGGGTAGCACATTGTCCCAACGGAGAAGATGAGAACCGTTGTGTTCGTCTCTACGGACAAAGCTTCATCCTCCAGGTTTACTCATCTCAGAGGAAAGCCTGGTATCCCGTGTGCCAGGATGATTGGAGTGAGAGCTACGGGAGAGCAGCATGTAAAGACATGGGATACAAGAACAATTTTTATTCTAGCCAAGGGATACCAGACCAGAGCGGGGCAACGAGCTTTATGAAGCTGAATGTGAGCTCAGGCAACGTTGACCTCTATAAAAAACTCTACCACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ATGTGAGCTCAGGCAACGTT
60.25
CAAGGTCCGATTCCTGGAGG
59.82
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AATGTGAGCTCAGGCAACGT
60.25
CAAGGTCCGATTCCTGGAGG
59.82
141
PCR template sequence:
ATTCTAGCCAAGGGATACCAGACCAGAGCGGGGCAACGAGCTTTATGAAGCTGAATGTGAGCTCAGGCAACGTTGACCTCTATAAAAAACTCTACCACAGGTCACCTGGAGGAAGCCCCATACTGACCTCCTCATGCTGCTGACACAGGCAGGATGGCATTGAACTCAGGGTCACCTCCAGGAATCGGACCTTGCTATGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
5
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-466a-5p(1), mmu-miR-1187(1), mmu-miR-466p-5p(1), mmu-miR-490-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AACTCTACCACAGGTCACCTGGAGGA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMPRSS2_733_ORF_1
0.69
0.108195009
0.84097762
723
ORF sequence:
ATGGCATTGAACTCAGGGTCACCTCCAGGAATCGGACCTTGCTATGAGAACCACGGGTATCAGTCTGAGCACATCTGTCCTCCGAGACCACCAGTGGCTCCCAATGGCTACAACTTGTATCCAGCCCAGTACTACCCATCTCCAGTGCCTCAGTATGCTCCGAGGATTACAACGCAAGCCTCAACATCTGTCATCCACACACATCCCAAGTCCTCAGGAGCACTGTGCACCTCAAAGTCTAAGAAATCGCTGTGTTTAGCCCTCGCCCTGGGCACTGTCCTCACGGGAGCTGCTGTGGCTGCTGTCTTGCTTTGGAGGTTCTGGGACAGCAACTGTTCTACGTCTGAGATGGAGTGTGGGTCTTCAGGCACATGCATCAGCTCTTCTCTCTGGTGTGACGGGGTAGCACATTGTCCCAACGGAGAAGATGAGAACCGTTGTGTTCGTCTCTACGGACAAAGCTTCATCCTCCAGGTTTACTCATCTCAGAGGAAAGCCTGGTATCCCGTGTGCCAGGATGATTGGAGTGAGAGCTACGGGAGAGCAGCATGTAAAGACATGGGATACAAGAACAATTTTTATTCTAGCCAAGGGATACCAGACCAGAGCGGGGCAACGAGCTTTATGAAGCTGAATGTGAGCTCAGGCAACGTTGACCTCTATAAAAAACTCTACCACAGGTCACCTGGAGGAAGCCCCATACTGACCTCCTCATGCTGCTGA
Peptide sequence:
MALNSGSPPGIGPCYENHGYQSEHICPPRPPVAPNGYNLYPAQYYPSPVPQYAPRITTQASTSVIHTHPKSSGALCTSKSKKSLCLALALGTVLTGAAVAAVLLWRFWDSNCSTSEMECGSSGTCISSSLWCDGVAHCPNGEDENRCVRLYGQSFILQVYSSQRKAWYPVCQDDWSESYGRAACKDMGYKNNFYSSQGIPDQSGATSFMKLNVSSGNVDLYKKLYHRSPGGSPILTSSCC
IRESfinder result
IRESfinder score
0.550050721
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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