top of page
mmu_circTmem67_1110
Basic information of circular RNA
PanCircBase ID:
mmu_circTmem67_1110
mm39_Chr. Position ID:
chr4|12051387|12063830|-
Gene name:
Tmem67
Transcript:
ENSMUST00000050686.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
21,20,19,18,17,16,15,14,13,12
Exon/intron offsets:
0,2059,2287,3358,3643,5928,7133,10367,11647,12286
Genomic length:
12443
Exon/intron sizes in nt:
141,140,100,87,99,99,57,106,124,157
Flanking exon/introns:
chr4:12047890-12051387|chr4:12063830-12068867
Splice length:
1110
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TGTGAAATCTCTCTCTCTAAACTCCTGGTTGACTTCAGCAGCCCTGTATTTTATGATGTTTACCTCGAATACACTGATGAAGAGCAGCACCGTTACCTTTGGCCTATCCCAGTGTTAAACCTAAATCTTCAACACAACAAACTGTTTGTGAACCAAGACAGTAGCTCCAGCAAGTGGCTTCTGACTCGGCGCATTTTCTTAGTGGATGCAGTAAGTGGGCGAGAAAATGACCTAGGAAATCAGCCAAGAGTAATTCGAGTTGCTACCCAGATTTCACTGAGCATACGCCTTGTACCCAACACAAAAAATGGAAACATATACACTCCCCTACTTACCATTGCCTACAGTGACATTGACATCAAAAATGCTCATAGCCAGTCTGCGAAGATTTCCTTCTCAGTGAAGTATGAGATGAATCAAGGAGACGCATCTGTGCACACAGATATTGCGTTGGGTGTGTTGGGTGGGCTGGCTGTGCTATCATCTCTTTTGAAGACAGCGGGATGGAAGAGGCGCATCGGGAGCCCCATGATTGATTTACAGACAGTTATGAAATTCCTGCTGTACTATGCTGGTGATCTGGCCAATGTGTTTTTTATCATCACAGTGGGAACAGGTCTTTATTGGCTTATTTTCTTCAAAGCACAGAAGTCAGTCTCTGTTTTGCTGCCGATGCCAGTTCAGGAAGAACGTTTCGTTACTTATGTGGGGTGCGCCTTTGCTATGAAGGCTCTCCAGTTTTTACATAAGTTCATATCCCAGATTTCCATAGATATATTCTTTATTGATTGGGAACGGCCGAAAGGAAAAGTTCTTAAAGCTGTTGAAGGTGAGGGTGGTGTGCGGAGTGCCACTGTTCCTGTGAGCATATGGAGAACGTATTTCGTGGCAAATGAATGGAATGAGATTCAGACTGTGAGAAAAATTAATCCACTCTTTCAAGTGCTTACCACACTCTTCTTCTTGGAGGTTGTGGGATTCAAAAATTTAGCGTTAATGGATTCATCCTCCAGCCTTTCTAGAAACCCATCAGACTACACAGCTCCTTACAGCCGCATTCTGAGATATGCCGTGGCTACTGCTATCTGGCTGGTGATTGGAATAATACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GATATGCCGTGGCTACTGCT
59.97
AACGGTGCTGCTCTTCATCA
59.96
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AGATATGCCGTGGCTACTGC
59.97
AACGGTGCTGCTCTTCATCA
59.96
142
PCR template sequence:
CAGCCTTTCTAGAAACCCATCAGACTACACAGCTCCTTACAGCCGCATTCTGAGATATGCCGTGGCTACTGCTATCTGGCTGGTGATTGGAATAATACAGTGTGAAATCTCTCTCTCTAAACTCCTGGTTGACTTCAGCAGCCCTGTATTTTATGATGTTTACCTCGAATACACTGATGAAGAGCAGCACCGTTACCTTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-30e-5p(1), mmu-miR-205-5p(1), mmu-miR-30a-5p(1), mmu-miR-30c-5p(1), mmu-miR-217-5p(1), mmu-miR-143-3p(1), mmu-miR-30d-5p(1), mmu-miR-30b-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-3470b(1), mmu-miR-574-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TGGAATAATACAGTGTGAAATCTCTC
siRNA sets
TTTCACACTGTATTATTCC, ATTTCACACTGTATTATTC, TTCACACTGTATTATTCCA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMEM67_1110_ORF_1
0.988
0.071389481
0.999949251
1809
ORF sequence:
ATGAATCAAGGAGACGCATCTGTGCACACAGATATTGCGTTGGGTGTGTTGGGTGGGCTGGCTGTGCTATCATCTCTTTTGAAGACAGCGGGATGGAAGAGGCGCATCGGGAGCCCCATGATTGATTTACAGACAGTTATGAAATTCCTGCTGTACTATGCTGGTGATCTGGCCAATGTGTTTTTTATCATCACAGTGGGAACAGGTCTTTATTGGCTTATTTTCTTCAAAGCACAGAAGTCAGTCTCTGTTTTGCTGCCGATGCCAGTTCAGGAAGAACGTTTCGTTACTTATGTGGGGTGCGCCTTTGCTATGAAGGCTCTCCAGTTTTTACATAAGTTCATATCCCAGATTTCCATAGATATATTCTTTATTGATTGGGAACGGCCGAAAGGAAAAGTTCTTAAAGCTGTTGAAGGTGAGGGTGGTGTGCGGAGTGCCACTGTTCCTGTGAGCATATGGAGAACGTATTTCGTGGCAAATGAATGGAATGAGATTCAGACTGTGAGAAAAATTAATCCACTCTTTCAAGTGCTTACCACACTCTTCTTCTTGGAGGTTGTGGGATTCAAAAATTTAGCGTTAATGGATTCATCCTCCAGCCTTTCTAGAAACCCATCAGACTACACAGCTCCTTACAGCCGCATTCTGAGATATGCCGTGGCTACTGCTATCTGGCTGGTGATTGGAATAATACAGTGTGAAATCTCTCTCTCTAAACTCCTGGTTGACTTCAGCAGCCCTGTATTTTATGATGTTTACCTCGAATACACTGATGAAGAGCAGCACCGTTACCTTTGGCCTATCCCAGTGTTAAACCTAAATCTTCAACACAACAAACTGTTTGTGAACCAAGACAGTAGCTCCAGCAAGTGGCTTCTGACTCGGCGCATTTTCTTAGTGGATGCAGTAAGTGGGCGAGAAAATGACCTAGGAAATCAGCCAAGAGTAATTCGAGTTGCTACCCAGATTTCACTGAGCATACGCCTTGTACCCAACACAAAAAATGGAAACATATACACTCCCCTACTTACCATTGCCTACAGTGACATTGACATCAAAAATGCTCATAGCCAGTCTGCGAAGATTTCCTTCTCAGTGAAGTATGAGATGAATCAAGGAGACGCATCTGTGCACACAGATATTGCGTTGGGTGTGTTGGGTGGGCTGGCTGTGCTATCATCTCTTTTGAAGACAGCGGGATGGAAGAGGCGCATCGGGAGCCCCATGATTGATTTACAGACAGTTATGAAATTCCTGCTGTACTATGCTGGTGATCTGGCCAATGTGTTTTTTATCATCACAGTGGGAACAGGTCTTTATTGGCTTATTTTCTTCAAAGCACAGAAGTCAGTCTCTGTTTTGCTGCCGATGCCAGTTCAGGAAGAACGTTTCGTTACTTATGTGGGGTGCGCCTTTGCTATGAAGGCTCTCCAGTTTTTACATAAGTTCATATCCCAGATTTCCATAGATATATTCTTTATTGATTGGGAACGGCCGAAAGGAAAAGTTCTTAAAGCTGTTGAAGGTGAGGGTGGTGTGCGGAGTGCCACTGTTCCTGTGAGCATATGGAGAACGTATTTCGTGGCAAATGAATGGAATGAGATTCAGACTGTGAGAAAAATTAATCCACTCTTTCAAGTGCTTACCACACTCTTCTTCTTGGAGGTTGTGGGATTCAAAAATTTAGCGTTAATGGATTCATCCTCCAGCCTTTCTAGAAACCCATCAGACTACACAGCTCCTTACAGCCGCATTCTGAGATATGCCGTGGCTACTGCTATCTGGCTGGTGATTGGAATAATACAG
Peptide sequence:
MNQGDASVHTDIALGVLGGLAVLSSLLKTAGWKRRIGSPMIDLQTVMKFLLYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPVQEERFVTYVGCAFAMKALQFLHKFISQISIDIFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINPLFQVLTTLFFLEVVGFKNLALMDSSSSLSRNPSDYTAPYSRILRYAVATAIWLVIGIIQCEISLSKLLVDFSSPVFYDVYLEYTDEEQHRYLWPIPVLNLNLQHNKLFVNQDSSSSKWLLTRRIFLVDAVSGRENDLGNQPRVIRVATQISLSIRLVPNTKNGNIYTPLLTIAYSDIDIKNAHSQSAKISFSVKYEMNQGDASVHTDIALGVLGGLAVLSSLLKTAGWKRRIGSPMIDLQTVMKFLLYYAGDLANVFFIITVGTGLYWLIFFKAQKSVSVLLPMPVQEERFVTYVGCAFAMKALQFLHKFISQISIDIFFIDWERPKGKVLKAVEGEGGVRSATVPVSIWRTYFVANEWNEIQTVRKINPLFQVLTTLFFLEVVGFKNLALMDSSSSLSRNPSDYTAPYSRILRYAVATAIWLVIGIIQ
IRESfinder result
IRESfinder score
0.784639477
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page