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mmu_circTmem63a_1157
Basic information of circular RNA
PanCircBase ID:
mmu_circTmem63a_1157
mm39_Chr. Position ID:
chr1|180787990|180797850|+
Gene name:
Tmem63a
Transcript:
ENSMUST00000027800.15
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
10
Exon/intron indexes:
10,11,12,13,14,15,16,17,18,19
Exon/intron offsets:
0,309,622,1921,2573,5114,5318,6042,8319,9754
Genomic length:
9860
Exon/intron sizes in nt:
81,12,41,34,13,81,54,10,00,00,00,00,000
Flanking exon/introns:
chr1:180785612-180787990|chr1:180797850-180799531
Splice length:
1157
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
AGATGCGTATCCCACGTGTGAGGTGGTGGATGTCCAGCTGTGCTACAGCGTGGCTAAGCTGATCTACCTGTGCAAAGAGAGAAAGAAGACCGAGAAGAGCCTTACCTATTACACAAACCTGCAGGCGAAGACAGGCCGACGGACCCTCATCAACCCCAAGCCTTGTGGCCAGTTCTGCTGCTGTGAAGTTCAGGGTTGTGAGCGGGAAGATGCTATTTCTTACTACACTCGAATGAATGACAGCCTGCTGGAGAGGATCACAGCCGAAGAGAGCCGGGTCCAGGACCAGCCCCTGGGAATGGCCTTTGTCACCTTCCGTGAGAAGTCCATGGCTACCTACATACTGAAAGACTTCAATGCCTGCAAGTGCCAGGGCCTTCGGTGCAAAGGGGAGCCCCAGCCATCCTCCTATAGCAGAGAGCTCTGTGTCTCCAAGTGGACGGTCACTTTTGCCTCCTATCCTGAGGACATCTGCTGGAAGAACCTCTCCATCCAGGGCGTTCGCTGGTGGCTCCAGTGGCTGGGTATCAACTTCTCTCTGTTTGTGGTGCTGTTTTTCCTGACCACGCCCTCCATCATTATGTCTACCATGGACAAGTTCAATGTTACCAAACCCATCCACGCACTGAATAACCCCGTCATCAGCCAGTTCTTCCCCACACTCCTGCTGTGGTCCTTCTCTGCGCTGCTTCCGTCCATTGTCTACTACTCCACGCTGCTGGAGTCCCACTGGACAAGGTCAGGGGAAAACCGGATCATGGTGTCGAAGGTCTACATATTTTTGATCTTCATGGTGCTGATCCTGCCCTCCCTCGGTCTCACCAGCTTGGATTTCTTCTTCCGGTGGCTCTTTGACAAAACTTCCTCGGAAACCTCAATCAGGCTAGAGTGTGTCTTCCTGCCAGACCAGGGTGCCTTCTTTGTGAACTACGTCATCGCCTCAGCCTTCATCGGCAGTGGCATGGAGCTGTTGCGCCTGCCCGGCCTCATCCTCTATACCTTCCGCATGATCATGGCCAAGACGGCCGCTGACCGCAGGAATGTTAAGCAGAACCAGGCTTTTGAATACGAGTTTGGAGCCATGTATGCGTGGATGCTGTGTGTCTTCACCGTCATCATGGCTTACAGCATCACCTGCCCCATTATCGTGCCCTTTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TATGCGTGGATGCTGTGTGT
60.04
GTAGATCAGCTTAGCCACGCT
59.93
140
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGTATGCGTGGATGCTGTGT
60.04
GTAGATCAGCTTAGCCACGCT
59.93
142
PCR template sequence:
GCTTTTGAATACGAGTTTGGAGCCATGTATGCGTGGATGCTGTGTGTCTTCACCGTCATCATGGCTTACAGCATCACCTGCCCCATTATCGTGCCCTTTGAGATGCGTATCCCACGTGTGAGGTGGTGGATGTCCAGCTGTGCTACAGCGTGGCTAAGCTGATCTACCTGTGCAAAGAGAGAAAGAAGACCGAGAAGAGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
11
Interacting miRNAs (#binding sites)
mmu-miR-503-5p(2), mmu-miR-15a-5p(1), mmu-miR-15b-5p(1), mmu-miR-29b-3p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-184-3p(1), mmu-miR-342-3p(1), mmu-miR-350-3p(1), mmu-miR-193b-3p(1), mmu-miR-202-3p(1)
siRNAs for circular RNA silencing
Junction sequence
ATCGTGCCCTTTGAGATGCGTATCCC
siRNA sets
GATACGCATCTCAAAGGGC, ATACGCATCTCAAAGGGCA, GGATACGCATCTCAAAGGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMEM63A_1157_ORF_1
1.2571
0.373154064
0.996627627
987
ORF sequence:
ATGAATGACAGCCTGCTGGAGAGGATCACAGCCGAAGAGAGCCGGGTCCAGGACCAGCCCCTGGGAATGGCCTTTGTCACCTTCCGTGAGAAGTCCATGGCTACCTACATACTGAAAGACTTCAATGCCTGCAAGTGCCAGGGCCTTCGGTGCAAAGGGGAGCCCCAGCCATCCTCCTATAGCAGAGAGCTCTGTGTCTCCAAGTGGACGGTCACTTTTGCCTCCTATCCTGAGGACATCTGCTGGAAGAACCTCTCCATCCAGGGCGTTCGCTGGTGGCTCCAGTGGCTGGGTATCAACTTCTCTCTGTTTGTGGTGCTGTTTTTCCTGACCACGCCCTCCATCATTATGTCTACCATGGACAAGTTCAATGTTACCAAACCCATCCACGCACTGAATAACCCCGTCATCAGCCAGTTCTTCCCCACACTCCTGCTGTGGTCCTTCTCTGCGCTGCTTCCGTCCATTGTCTACTACTCCACGCTGCTGGAGTCCCACTGGACAAGGTCAGGGGAAAACCGGATCATGGTGTCGAAGGTCTACATATTTTTGATCTTCATGGTGCTGATCCTGCCCTCCCTCGGTCTCACCAGCTTGGATTTCTTCTTCCGGTGGCTCTTTGACAAAACTTCCTCGGAAACCTCAATCAGGCTAGAGTGTGTCTTCCTGCCAGACCAGGGTGCCTTCTTTGTGAACTACGTCATCGCCTCAGCCTTCATCGGCAGTGGCATGGAGCTGTTGCGCCTGCCCGGCCTCATCCTCTATACCTTCCGCATGATCATGGCCAAGACGGCCGCTGACCGCAGGAATGTTAAGCAGAACCAGGCTTTTGAATACGAGTTTGGAGCCATGTATGCGTGGATGCTGTGTGTCTTCACCGTCATCATGGCTTACAGCATCACCTGCCCCATTATCGTGCCCTTTGAGATGCGTATCCCACGTGTGAGGTGGTGGATGTCCAGCTGTGCTACAGCGTGGCTAAGCTGA
Peptide sequence:
MNDSLLERITAEESRVQDQPLGMAFVTFREKSMATYILKDFNACKCQGLRCKGEPQPSSYSRELCVSKWTVTFASYPEDICWKNLSIQGVRWWLQWLGINFSLFVVLFFLTTPSIIMSTMDKFNVTKPIHALNNPVISQFFPTLLLWSFSALLPSIVYYSTLLESHWTRSGENRIMVSKVYIFLIFMVLILPSLGLTSLDFFFRWLFDKTSSETSIRLECVFLPDQGAFFVNYVIASAFIGSGMELLRLPGLILYTFRMIMAKTAADRRNVKQNQAFEYEFGAMYAWMLCVFTVIMAYSITCPIIVPFEMRIPRVRWWMSSCATAWLS
IRESfinder result
IRESfinder score
0.452900003
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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