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mmu_circTmem255a_690
Basic information of circular RNA
PanCircBase ID:
mmu_circTmem255a_690
mm39_Chr. Position ID:
chrX|37296886|37324780|-
Gene name:
Tmem255a
Transcript:
ENSMUST00000089056.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
9,8,7,6,5,4,3
Exon/intron offsets:
0,7168,16571,17044,18878,25093,27831
Genomic length:
27894
Exon/intron sizes in nt:
144,163,89,72,69,90,63
Flanking exon/introns:
chrX:37286175-37296886|chrX:37324780-37334592
Splice length:
690
Other database IDs:
circAtlas: mmu-Tmem255a_0002
circAtlas: hsa-TMEM255A_0004, CIRCpedia: HSA_CIRCpedia_65101
Human Ortholog IDs:
Mature spliced Sequence:
CTTGGCTTTGGATCATTCCTTGGGATCATCGGGTCAAACCTGATTGAGAACAAGCGGCAGATGCTGGTGGCTTCTATCGTGTTTATCAGCTTTGGTGTGATTGCAGCATTTTGTTGTGCTATAGTTGATGGGGTCTTTGCCGCCAGACACATTGATTTGAAACCACTCTATGCTAACCGGTGCCACTATGTTCCCAAGACATCACAGAGGGAAGCTGAGGAGGTCATAACTTCCTCATCCAAAATTACTCCTTCTACGAGGGCTTTGAGGAACCTTACCCAGGCAGTTAAAGAGGTGAACTGCCCTCAACTTAGCCGTGGACTTTGCACACCTCGCATCCGGGGCAACACCTGCTTCTGCTGTGACCTCTACAACTGTGGCAACAGGGTGGAAATCACCGGCGGCTACTATGAGTACATTGATGTCAGCAGCTGCCAGGACATCATCCACCTGTACCACCTACTCTGGTCTGCCACCATCCTCAACATCGTTGGCCTGTTCCTGGGTATCATTACTGCTGCGGTCCTTGGGGGATTTAAGGATATGAACCCTACTCTTCCGGCACTGAACTGCTCTGTTGAAAATGCCCATCCAACTGTTTCTTACTATGCGCGTCCCCAAGTGGCATCGTACAATACCTACTACCATAGCCCTCCTCATCTGCCACCGTATTCTGCTTATGACTTTCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTTACTATGCGCGTCCCC
59.9
ACACGATAGAAGCCACCAGC
60.11
173
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTCTTACTATGCGCGTCCCC
59.9
CACGATAGAAGCCACCAGCA
60.11
172
PCR template sequence:
TCCAACTGTTTCTTACTATGCGCGTCCCCAAGTGGCATCGTACAATACCTACTACCATAGCCCTCCTCATCTGCCACCGTATTCTGCTTATGACTTTCAGCTTGGCTTTGGATCATTCCTTGGGATCATCGGGTCAAACCTGATTGAGAACAAGCGGCAGATGCTGGTGGCTTCTATCGTGTTTATCAGCTTTGGTGTGA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
10
Interacting miRNAs (#binding sites)
mmu-miR-140-5p(1), mmu-miR-19b-3p(1), mmu-miR-211-5p(1), mmu-miR-19a-3p(1), mmu-miR-10b-5p(1), mmu-miR-10a-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-3065-5p(1), mmu-miR-376a-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
TTATGACTTTCAGCTTGGCTTTGGAT
siRNA sets
AAAGCCAAGCTGAAAGTCA,AAGCCAAGCTGAAAGTCAT,CAAAGCCAAGCTGAAAGTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMEM255A_690_ORF_1
0.9407
0.210803791
0.998953682
1320
ORF sequence:
ATGCTGGTGGCTTCTATCGTGTTTATCAGCTTTGGTGTGATTGCAGCATTTTGTTGTGCTATAGTTGATGGGGTCTTTGCCGCCAGACACATTGATTTGAAACCACTCTATGCTAACCGGTGCCACTATGTTCCCAAGACATCACAGAGGGAAGCTGAGGAGGTCATAACTTCCTCATCCAAAATTACTCCTTCTACGAGGGCTTTGAGGAACCTTACCCAGGCAGTTAAAGAGGTGAACTGCCCTCAACTTAGCCGTGGACTTTGCACACCTCGCATCCGGGGCAACACCTGCTTCTGCTGTGACCTCTACAACTGTGGCAACAGGGTGGAAATCACCGGCGGCTACTATGAGTACATTGATGTCAGCAGCTGCCAGGACATCATCCACCTGTACCACCTACTCTGGTCTGCCACCATCCTCAACATCGTTGGCCTGTTCCTGGGTATCATTACTGCTGCGGTCCTTGGGGGATTTAAGGATATGAACCCTACTCTTCCGGCACTGAACTGCTCTGTTGAAAATGCCCATCCAACTGTTTCTTACTATGCGCGTCCCCAAGTGGCATCGTACAATACCTACTACCATAGCCCTCCTCATCTGCCACCGTATTCTGCTTATGACTTTCAGCTTGGCTTTGGATCATTCCTTGGGATCATCGGGTCAAACCTGATTGAGAACAAGCGGCAGATGCTGGTGGCTTCTATCGTGTTTATCAGCTTTGGTGTGATTGCAGCATTTTGTTGTGCTATAGTTGATGGGGTCTTTGCCGCCAGACACATTGATTTGAAACCACTCTATGCTAACCGGTGCCACTATGTTCCCAAGACATCACAGAGGGAAGCTGAGGAGGTCATAACTTCCTCATCCAAAATTACTCCTTCTACGAGGGCTTTGAGGAACCTTACCCAGGCAGTTAAAGAGGTGAACTGCCCTCAACTTAGCCGTGGACTTTGCACACCTCGCATCCGGGGCAACACCTGCTTCTGCTGTGACCTCTACAACTGTGGCAACAGGGTGGAAATCACCGGCGGCTACTATGAGTACATTGATGTCAGCAGCTGCCAGGACATCATCCACCTGTACCACCTACTCTGGTCTGCCACCATCCTCAACATCGTTGGCCTGTTCCTGGGTATCATTACTGCTGCGGTCCTTGGGGGATTTAAGGATATGAACCCTACTCTTCCGGCACTGAACTGCTCTGTTGAAAATGCCCATCCAACTGTTTCTTACTATGCGCGTCCCCAAGTGGCATCGTACAATACCTACTACCATAGCCCTCCTCATCTGCCACCGTATTCTGCTTATGACTTTCAG
Peptide sequence:
MLVASIVFISFGVIAAFCCAIVDGVFAARHIDLKPLYANRCHYVPKTSQREAEEVITSSSKITPSTRALRNLTQAVKEVNCPQLSRGLCTPRIRGNTCFCCDLYNCGNRVEITGGYYEYIDVSSCQDIIHLYHLLWSATILNIVGLFLGIITAAVLGGFKDMNPTLPALNCSVENAHPTVSYYARPQVASYNTYYHSPPHLPPYSAYDFQLGFGSFLGIIGSNLIENKRQMLVASIVFISFGVIAAFCCAIVDGVFAARHIDLKPLYANRCHYVPKTSQREAEEVITSSSKITPSTRALRNLTQAVKEVNCPQLSRGLCTPRIRGNTCFCCDLYNCGNRVEITGGYYEYIDVSSCQDIIHLYHLLWSATILNIVGLFLGIITAAVLGGFKDMNPTLPALNCSVENAHPTVSYYARPQVASYNTYYHSPPHLPPYSAYDFQ
IRESfinder result
IRESfinder score
0.657432787
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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