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mmu_circTmem175_380
Basic information of circular RNA
PanCircBase ID:
mmu_circTmem175_380
mm39_Chr. Position ID:
chr5|108790904|108792558|+
Gene name:
Tmem175
Transcript:
ENSMUST00000063272.13
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
8,9,10
Exon/intron offsets:
0,1129,1518
Genomic length:
1654
Exon/intron sizes in nt:
1,65,79,136
Flanking exon/introns:
chr5:108790645-108790904|chr5:108792558-108793704
Splice length:
380
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
GCAATGATTGTGGGCTATGCCTTCCACTTCCCACATCTGCTGAATCCACAAATCCAGTGCTCTACACATAGGGATCTATCCCGGCGGCATATCCTGCACCTGGTCCTCCGTGGCCCAGCACTGTGTTTTGTTGCGGCTGTCTTTTCCCTCTTCTTCTTCCCCTTGTCATACCTGCTGATGGTGACTGTCATCTTCCTCCCTCACATCAGCAAGGCTACCACCTGGTGCAAAGACAAGTTGATGGGCCAAAGGGAGTCTCCAGCTCACGACATGGAGCCCTTCAGTATTGACCTGCATGCGCCCCTCAGCAAAGAGCGGGTGGAAGCTTTCAGCGACGGTGTCTATGCCATCGTGGCCACACTCCTCATCCTGGACATCTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CAGTATTGACCTGCATGCGC
59.97
CAGCAGATGTGGGAAGTGGA
59.67
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CAGTATTGACCTGCATGCGC
59.97
TCAGCAGATGTGGGAAGTGG
59.67
142
PCR template sequence:
TCAGTATTGACCTGCATGCGCCCCTCAGCAAAGAGCGGGTGGAAGCTTTCAGCGACGGTGTCTATGCCATCGTGGCCACACTCCTCATCCTGGACATCTGGCAATGATTGTGGGCTATGCCTTCCACTTCCCACATCTGCTGAATCCACAAATCCAGTGCTCTACACATAGGGATCTATCCCGGCGGCATATCCTGCACC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-200c-3p(1), mmu-miR-122-5p(1), mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-214-3p(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TCCTGGACATCTGGCAATGATTGTGG
siRNA sets
CAATCATTGCCAGATGTCC, AATCATTGCCAGATGTCCA, ATCATTGCCAGATGTCCAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMEM175_380_ORF_1
1.1497
0.274526964
0.683661202
384
ORF sequence:
ATGATTGTGGGCTATGCCTTCCACTTCCCACATCTGCTGAATCCACAAATCCAGTGCTCTACACATAGGGATCTATCCCGGCGGCATATCCTGCACCTGGTCCTCCGTGGCCCAGCACTGTGTTTTGTTGCGGCTGTCTTTTCCCTCTTCTTCTTCCCCTTGTCATACCTGCTGATGGTGACTGTCATCTTCCTCCCTCACATCAGCAAGGCTACCACCTGGTGCAAAGACAAGTTGATGGGCCAAAGGGAGTCTCCAGCTCACGACATGGAGCCCTTCAGTATTGACCTGCATGCGCCCCTCAGCAAAGAGCGGGTGGAAGCTTTCAGCGACGGTGTCTATGCCATCGTGGCCACACTCCTCATCCTGGACATCTGGCAATGA
Peptide sequence:
MIVGYAFHFPHLLNPQIQCSTHRDLSRRHILHLVLRGPALCFVAAVFSLFFFPLSYLLMVTVIFLPHISKATTWCKDKLMGQRESPAHDMEPFSIDLHAPLSKERVEAFSDGVYAIVATLLILDIWQ
IRESfinder result
IRESfinder score
0.503843188
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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