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mmu_circTmem131l_419
Basic information of circular RNA
PanCircBase ID:
mmu_circTmem131l_419
mm39_Chr. Position ID:
chr3|83844728|83846052|-
Gene name:
Tmem131l
Transcript:
ENSMUST00000191758.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
14,13,12
Exon/intron offsets:
0,592,1230
Genomic length:
1324
Exon/intron sizes in nt:
171,154,94
Flanking exon/introns:
chr3:83843373-83844728|chr3:83846052-83847803
Splice length:
419
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CAGGTACCTCGTGTGACAGTGGAATCATGGGCCTGAGGAAAAAAAAAGCTAGCCCAGCAATGCAAGCCTGCCTGTCCTCTCCTGTGGTGCAGGGGTATTTCAGAACGGATGCCTCTACAGCCCAGTTTCACATAGAGAGTCACGAGACTGCAACAGGAGTCTGGTCAATATGGTACCGCAGCCATTTTGACCAGAGCATTGTATTAAAAGATGTGTTTGTTTCCAAGGAGACCAAGCACATTTTAAAGGTTCTGAGTTTCCGAGGCCCTTTATTTCTACCCCCAGGCTGTTGGAATATATTTTCTCTGAAGCTTGCTGTTAAAGGCATTGTCTTAAACCTGTTCACCAATGTATTTCTGACTACAAACACAGGTGCCATTTTTGCAATACCTCTGCAGATTTTCTCGGCCCCAACCAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACACAGGTGCCATTTTTGCA
59.17
GCACCACAGGAGAGGACAG
59.71
143
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACACAGGTGCCATTTTTGCA
59.17
CTGCACCACAGGAGAGGAC
59.71
145
PCR template sequence:
TAAAGGCATTGTCTTAAACCTGTTCACCAATGTATTTCTGACTACAAACACAGGTGCCATTTTTGCAATACCTCTGCAGATTTTCTCGGCCCCAACCAAGCAGGTACCTCGTGTGACAGTGGAATCATGGGCCTGAGGAAAAAAAAAGCTAGCCCAGCAATGCAAGCCTGCCTGTCCTCTCCTGTGGTGCAGGGGTATTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-218-5p(1), mmu-miR-183-5p(1), mmu-miR-532-5p(1), mmu-miR-202-3p(1)
siRNAs for circular RNA silencing
Junction sequence
GGCCCCAACCAAGCAGGTACCTCGTG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMEM131L_419_ORF_1
0.7138
-0.009216606
0.29376146
429
ORF sequence:
ATGGGCCTGAGGAAAAAAAAAGCTAGCCCAGCAATGCAAGCCTGCCTGTCCTCTCCTGTGGTGCAGGGGTATTTCAGAACGGATGCCTCTACAGCCCAGTTTCACATAGAGAGTCACGAGACTGCAACAGGAGTCTGGTCAATATGGTACCGCAGCCATTTTGACCAGAGCATTGTATTAAAAGATGTGTTTGTTTCCAAGGAGACCAAGCACATTTTAAAGGTTCTGAGTTTCCGAGGCCCTTTATTTCTACCCCCAGGCTGTTGGAATATATTTTCTCTGAAGCTTGCTGTTAAAGGCATTGTCTTAAACCTGTTCACCAATGTATTTCTGACTACAAACACAGGTGCCATTTTTGCAATACCTCTGCAGATTTTCTCGGCCCCAACCAAGCAGGTACCTCGTGTGACAGTGGAATCATGGGCCTGA
Peptide sequence:
MGLRKKKASPAMQACLSSPVVQGYFRTDASTAQFHIESHETATGVWSIWYRSHFDQSIVLKDVFVSKETKHILKVLSFRGPLFLPPGCWNIFSLKLAVKGIVLNLFTNVFLTTNTGAIFAIPLQIFSAPTKQVPRVTVESWA
IRESfinder result
IRESfinder score
0.717171846
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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