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mmu_circTmem131_663
Basic information of circular RNA
PanCircBase ID:
mmu_circTmem131_663
mm39_Chr. Position ID:
chr1|36854535|36858296|-
Gene name:
Tmem131
Transcript:
ENSMUST00000027290.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
25,24,23,22,21,20
Exon/intron offsets:
0,432,654,3203,3438,3608
Genomic length:
3761
Exon/intron sizes in nt:
6,71,10,13,91,18,76,150
Flanking exon/introns:
chr1:36851789-36854535|chr1:36858296-36862147
Splice length:
663
Other database IDs:
CIRCpedia: MMU_CIRCpedia_18215, circAtlas: mmu-Tmem131_0016
Human Ortholog IDs:
Mature spliced Sequence:
GGGAAAATAGTTCATCAGAGCTTAAATATTATGAATTCCTTCTCACAGAAGGTGAAGATACAGCAAATAAGGTCTTTGTCAGAAGACGTACGATTCTACTACAAACGATTACGGGGTAATAGGGAAGACTTGGAGCCAGGGAAGAAGTCTAAGATTGCAAACATTTATTTTGACCCTGGGCTGCAGTGTGGGGATCACTGCTATATCGGCTTGCCTTTCCTATCCAAATCTGAACCCAAAGTACAGCCTGGGGTGGCCATGCAGGAGGACTTGTGGGATGCCGACTGGGACGCACATCAGAGCTTGTTCAAGGCTTGGATGGGGATAAAGGAAAACGCAGGACATAGGTTGAATGCTATGTTTGAAGTAAATACAGATCTTCAGAAAAATATCGTATCAAAAGTCAGTGCCGAGCTCTCCTGGCCGTCTGTCCTTAGCTCGCCCCGGCTCCTGAAGTTCCCACTCACTAACACAAACTGTTCTTCGGAAGAAGAGATTAGCTTAGAAAACCCTGCAGACGTCCCTGTCTACGTCCAGTTCATCCCACTGGCTTTGTATTCCAACCCCTCCGTCTTTGCAGATAAGTTAGTCTCAAGGTTTAACTTGAGTAAGGTGGCCAAGCTAGATTTGAGAACACTTGAATTCCAAGTCTACAGGAACAGC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CCCCTCCGTCTTTGCAGATA
59.17
TGCTGTATCTTCACCTTCTGTG
58.07
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGAGTAAGGTGGCCAAGCT
59.23
TCGTACGTCTTCTGACAAAGA
56.75
153
PCR template sequence:
CCCCTCCGTCTTTGCAGATAAGTTAGTCTCAAGGTTTAACTTGAGTAAGGTGGCCAAGCTAGATTTGAGAACACTTGAATTCCAAGTCTACAGGAACAGCGGGAAAATAGTTCATCAGAGCTTAAATATTATGAATTCCTTCTCACAGAAGGTGAAGATACAGCAAATAAGGTCTTTGTCAGAAGACGTACGATTCTACT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-210-3p(1), mmu-miR-324-5p(1), mmu-miR-149-5p(1)
siRNAs for circular RNA silencing
Junction sequence
CTACAGGAACAGCGGGAAAATAGTTC
siRNA sets
TTTCCCGCTGTTCCTGTAG, TATTTTCCCGCTGTTCCTG, ACTATTTTCCCGCTGTTCC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMEM131_663_ORF_1
0.993
0.150983047
0.998560742
1296
ORF sequence:
ATGAATTCCTTCTCACAGAAGGTGAAGATACAGCAAATAAGGTCTTTGTCAGAAGACGTACGATTCTACTACAAACGATTACGGGGTAATAGGGAAGACTTGGAGCCAGGGAAGAAGTCTAAGATTGCAAACATTTATTTTGACCCTGGGCTGCAGTGTGGGGATCACTGCTATATCGGCTTGCCTTTCCTATCCAAATCTGAACCCAAAGTACAGCCTGGGGTGGCCATGCAGGAGGACTTGTGGGATGCCGACTGGGACGCACATCAGAGCTTGTTCAAGGCTTGGATGGGGATAAAGGAAAACGCAGGACATAGGTTGAATGCTATGTTTGAAGTAAATACAGATCTTCAGAAAAATATCGTATCAAAAGTCAGTGCCGAGCTCTCCTGGCCGTCTGTCCTTAGCTCGCCCCGGCTCCTGAAGTTCCCACTCACTAACACAAACTGTTCTTCGGAAGAAGAGATTAGCTTAGAAAACCCTGCAGACGTCCCTGTCTACGTCCAGTTCATCCCACTGGCTTTGTATTCCAACCCCTCCGTCTTTGCAGATAAGTTAGTCTCAAGGTTTAACTTGAGTAAGGTGGCCAAGCTAGATTTGAGAACACTTGAATTCCAAGTCTACAGGAACAGCGGGAAAATAGTTCATCAGAGCTTAAATATTATGAATTCCTTCTCACAGAAGGTGAAGATACAGCAAATAAGGTCTTTGTCAGAAGACGTACGATTCTACTACAAACGATTACGGGGTAATAGGGAAGACTTGGAGCCAGGGAAGAAGTCTAAGATTGCAAACATTTATTTTGACCCTGGGCTGCAGTGTGGGGATCACTGCTATATCGGCTTGCCTTTCCTATCCAAATCTGAACCCAAAGTACAGCCTGGGGTGGCCATGCAGGAGGACTTGTGGGATGCCGACTGGGACGCACATCAGAGCTTGTTCAAGGCTTGGATGGGGATAAAGGAAAACGCAGGACATAGGTTGAATGCTATGTTTGAAGTAAATACAGATCTTCAGAAAAATATCGTATCAAAAGTCAGTGCCGAGCTCTCCTGGCCGTCTGTCCTTAGCTCGCCCCGGCTCCTGAAGTTCCCACTCACTAACACAAACTGTTCTTCGGAAGAAGAGATTAGCTTAGAAAACCCTGCAGACGTCCCTGTCTACGTCCAGTTCATCCCACTGGCTTTGTATTCCAACCCCTCCGTCTTTGCAGATAAGTTAGTCTCAAGGTTTAACTTGAGTAAGGTGGCCAAGCTAGATTTGAGAACACTTGAATTCCAAGTCTACAGGAACAGC
Peptide sequence:
MNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNSGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSEPKVQPGVAMQEDLWDADWDAHQSLFKAWMGIKENAGHRLNAMFEVNTDLQKNIVSKVSAELSWPSVLSSPRLLKFPLTNTNCSSEEEISLENPADVPVYVQFIPLALYSNPSVFADKLVSRFNLSKVAKLDLRTLEFQVYRNS
IRESfinder result
IRESfinder score
0.660404942
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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