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mmu_circTmem131_556
Basic information of circular RNA
PanCircBase ID:
mmu_circTmem131_556
mm39_Chr. Position ID:
chr1|36857973|36864427|-
Gene name:
Tmem131
Transcript:
ENSMUST00000027290.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
21,20,19,18,17
Exon/intron offsets:
0,170,4174,5823,6319
Genomic length:
6454
Exon/intron sizes in nt:
76,15,38,71,05,135
Flanking exon/introns:
chr1:36857856-36857973|chr1:36864427-36864509
Splice length:
556
Other database IDs:
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
CTGGCAATAAAAAGTTGGCATATCATAGGAGATGGCTTATCAATAGAACTTGTAGCTACAGAGAGAGGCAACAGAAGCACGGTGATTGCAAGCCTTCCAGAACTGGAGCGGTCCTCTTTGCCCGACCAGTCCCCGGTAACATTAGCTTCAGGACACTTTGCAGTGTTCAGAGTCAAACTTACTGCCAAGAAGCTAGAAGGGGTCCATGATGGGGCCATACAGATCACAACCGACTACGAGATCCTGACCATCCCTGTGAAAGCTGTGATCGCCGTGGGCTCGCTGACCTGCTTCCCCAAGCACATGGTTCTTCCCCCTTCCTTCCCAGGGAAAATAGTTCATCAGAGCTTAAATATTATGAATTCCTTCTCACAGAAGGTGAAGATACAGCAAATAAGGTCTTTGTCAGAAGACGTACGATTCTACTACAAACGATTACGGGGTAATAGGGAAGACTTGGAGCCAGGGAAGAAGTCTAAGATTGCAAACATTTATTTTGACCCTGGGCTGCAGTGTGGGGATCACTGCTATATCGGCTTGCCTTTCCTATCCAAAT
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGGAGCCAGGGAAGAAGTCT
60.18
CGTGCTTCTGTTGCCTCTCT
60.32
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TATTTTGACCCTGGGCTGCA
59.59
GAAGGCTTGCAATCACCGTG
60.11
161
PCR template sequence:
TTGGAGCCAGGGAAGAAGTCTAAGATTGCAAACATTTATTTTGACCCTGGGCTGCAGTGTGGGGATCACTGCTATATCGGCTTGCCTTTCCTATCCAAATCTGGCAATAAAAAGTTGGCATATCATAGGAGATGGCTTATCAATAGAACTTGTAGCTACAGAGAGAGGCAACAGAAGCACGGTGATTGCAAGCCTTCCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-149-5p(1)
siRNAs for circular RNA silencing
Junction sequence
TTCCTATCCAAATCTGGCAATAAAAA
siRNA sets
TTTTTATTGCCAGATTTGG, TTGCCAGATTTGGATAGGA, ATTGCCAGATTTGGATAGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTMEM131_556_ORF_1
0.5174
-0.030662155
0.250043565
462
ORF sequence:
ATGGTTCTTCCCCCTTCCTTCCCAGGGAAAATAGTTCATCAGAGCTTAAATATTATGAATTCCTTCTCACAGAAGGTGAAGATACAGCAAATAAGGTCTTTGTCAGAAGACGTACGATTCTACTACAAACGATTACGGGGTAATAGGGAAGACTTGGAGCCAGGGAAGAAGTCTAAGATTGCAAACATTTATTTTGACCCTGGGCTGCAGTGTGGGGATCACTGCTATATCGGCTTGCCTTTCCTATCCAAATCTGGCAATAAAAAGTTGGCATATCATAGGAGATGGCTTATCAATAGAACTTGTAGCTACAGAGAGAGGCAACAGAAGCACGGTGATTGCAAGCCTTCCAGAACTGGAGCGGTCCTCTTTGCCCGACCAGTCCCCGGTAACATTAGCTTCAGGACACTTTGCAGTGTTCAGAGTCAAACTTACTGCCAAGAAGCTAGAAGGGGTCCATGA
Peptide sequence:
MVLPPSFPGKIVHQSLNIMNSFSQKVKIQQIRSLSEDVRFYYKRLRGNREDLEPGKKSKIANIYFDPGLQCGDHCYIGLPFLSKSGNKKLAYHRRWLINRTCSYRERQQKHGDCKPSRTGAVLFARPVPGNISFRTLCSVQSQTYCQEARRGP
IRESfinder result
IRESfinder score
0.617800814
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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