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mmu_circTjp2_282
Basic information of circular RNA
PanCircBase ID:
mmu_circTjp2_282
mm39_Chr. Position ID:
chr19|24109969|24116196|-
Gene name:
Tjp2
Transcript:
ENSMUST00000233027.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
4,3,2
Exon/intron offsets:
0,2244,6173
Genomic length:
6227
Exon/intron sizes in nt:
103,125,54
Flanking exon/introns:
chr19:24108837-24109969|chr19:24116196-24151318
Splice length:
282
Other database IDs:
CIRCpedia: MMU_CIRCpedia_14064, circAtlas: mmu-Tjp2_0004
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_111423
Human Ortholog IDs:
Mature spliced Sequence:
GGTCCAGGCATGGAGGAGGTGATATGGGAGCAGTACACCGTGACCCTGCAGAAGGATTCCAAAAGAGGATTTGGAATTGCCGTGTCGGGAGGCAGAGACAACCCCCACTTTGAAAATGGGGAGACGTCAATCGTCATCTCAGATGTGCTCCCAGGTGGACCCGCTGATGGCTTGCTTCAAGAAAATGACAGGGTGGTCATGGTCAATGGCACCCCCATGGAAGATGTGCTCCATTCGTTTGCCGTTCAGCAGCTTAGGAAAAGTGGGAAGATAGCAGCCATC
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGGGTGGTCATGGTCAATGG
59.67
AATCCTTCTGCAGGGTCACG
60.04
151
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACAGGGTGGTCATGGTCAA
59.6
AATCCTTCTGCAGGGTCACG
60.04
154
PCR template sequence:
AAATGACAGGGTGGTCATGGTCAATGGCACCCCCATGGAAGATGTGCTCCATTCGTTTGCCGTTCAGCAGCTTAGGAAAAGTGGGAAGATAGCAGCCATCGGTCCAGGCATGGAGGAGGTGATATGGGAGCAGTACACCGTGACCCTGCAGAAGGATTCCAAAAGAGGATTTGGAATTGCCGTGTCGGGAGGCAGAGACA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-342-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GATAGCAGCCATCGGTCCAGGCATGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTJP2_282_ORF_1
1.1556
0.378886465
0.920332111
555
ORF sequence:
ATGGAGGAGGTGATATGGGAGCAGTACACCGTGACCCTGCAGAAGGATTCCAAAAGAGGATTTGGAATTGCCGTGTCGGGAGGCAGAGACAACCCCCACTTTGAAAATGGGGAGACGTCAATCGTCATCTCAGATGTGCTCCCAGGTGGACCCGCTGATGGCTTGCTTCAAGAAAATGACAGGGTGGTCATGGTCAATGGCACCCCCATGGAAGATGTGCTCCATTCGTTTGCCGTTCAGCAGCTTAGGAAAAGTGGGAAGATAGCAGCCATCGGTCCAGGCATGGAGGAGGTGATATGGGAGCAGTACACCGTGACCCTGCAGAAGGATTCCAAAAGAGGATTTGGAATTGCCGTGTCGGGAGGCAGAGACAACCCCCACTTTGAAAATGGGGAGACGTCAATCGTCATCTCAGATGTGCTCCCAGGTGGACCCGCTGATGGCTTGCTTCAAGAAAATGACAGGGTGGTCATGGTCAATGGCACCCCCATGGAAGATGTGCTCCATTCGTTTGCCGTTCAGCAGCTTAGGAAAAGTGGGAAGATAGCAGCCATC
Peptide sequence:
MEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAI
IRESfinder result
IRESfinder score
0.362077612
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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