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mmu_circTial1_575
Basic information of circular RNA
PanCircBase ID:
mmu_circTial1_575
mm39_Chr. Position ID:
chr7|128047374|128056756|-
Gene name:
Tial1
Transcript:
ENSMUST00000106226.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
6
Exon/intron indexes:
7,6,5,4,3,2
Exon/intron offsets:
0,544,2470,2691,2970,9285
Genomic length:
9382
Exon/intron sizes in nt:
109,76,88,55,150,97
Flanking exon/introns:
chr7:128046631-128047374|chr7:128056756-128062848
Splice length:
575
Other database IDs:
circAtlas: mmu-Tial1_0008
circBase: hsa_circ_0020181, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_635
Human Ortholog IDs:
Mature spliced Sequence:
ATACGTAGGTAACCTCTCCAGAGATGTGACAGAAGTCCTTATTCTTCAGTTATTCAGTCAGATCGGACCCTGTAAAAGCTGTAAAATGATAACAGAGCAACCCGATAGCAGAAGGGTCAACTCTTCTGTTGGATTTTCTGTTTTGCAGCATACAAGCAATGACCCATATTGCTTTGTGGAATTTTATGAACACAGAGATGCAGCTGCTGCATTAGCTGCTATGAATGGGAGAAAAATTTTGGGAAAGGAGGTCAAAGTAAACTGGGCAACAACACCAAGTAGCCAGAAAAAAGATACTTCCAATCACTTCCATGTGTTTGTTGGGGATTTGAGTCCAGAAATTACAACAGAAGATATCAAATCAGCATTTGCCCCCTTTGGTAAAATATCGGATGCCCGGGTAGTTAAAGACATGGCAACTGGAAAGTCCAAAGGCTATGGTTTTGTATCTTTTTATAACAAACTGGATGCAGAGAATGCAATTGTGCATATGGGGGGTCAGTGGTTGGGAGGTCGACAAATCAGAACCAATTGGGCCACACGTAAACCACCTGCCCCTAAAAGTACACAAGAAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGTTGGGAGGTCGACAAA
60.18
ACAGGGTCCGATCTGACTGA
59.96
147
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGTTGGGAGGTCGACAAAT
59.23
ACAGGGTCCGATCTGACTGA
59.96
146
PCR template sequence:
AATGCAATTGTGCATATGGGGGGTCAGTGGTTGGGAGGTCGACAAATCAGAACCAATTGGGCCACACGTAAACCACCTGCCCCTAAAAGTACACAAGAAAATACGTAGGTAACCTCTCCAGAGATGTGACAGAAGTCCTTATTCTTCAGTTATTCAGTCAGATCGGACCCTGTAAAAGCTGTAAAATGATAACAGAGCAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-150-5p(1), mmu-miR-495-3p(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGTACACAAGAAAATACGTAGGTAAC
siRNA sets
TTACCTACGTATTTTCTTG, TACCTACGTATTTTCTTGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTIAL1_575_ORF_1
1.0544
0.003421265
0.574087352
498
ORF sequence:
ATGATAACAGAGCAACCCGATAGCAGAAGGGTCAACTCTTCTGTTGGATTTTCTGTTTTGCAGCATACAAGCAATGACCCATATTGCTTTGTGGAATTTTATGAACACAGAGATGCAGCTGCTGCATTAGCTGCTATGAATGGGAGAAAAATTTTGGGAAAGGAGGTCAAAGTAAACTGGGCAACAACACCAAGTAGCCAGAAAAAAGATACTTCCAATCACTTCCATGTGTTTGTTGGGGATTTGAGTCCAGAAATTACAACAGAAGATATCAAATCAGCATTTGCCCCCTTTGGTAAAATATCGGATGCCCGGGTAGTTAAAGACATGGCAACTGGAAAGTCCAAAGGCTATGGTTTTGTATCTTTTTATAACAAACTGGATGCAGAGAATGCAATTGTGCATATGGGGGGTCAGTGGTTGGGAGGTCGACAAATCAGAACCAATTGGGCCACACGTAAACCACCTGCCCCTAAAAGTACACAAGAAAATACGTAG
Peptide sequence:
MITEQPDSRRVNSSVGFSVLQHTSNDPYCFVEFYEHRDAAAALAAMNGRKILGKEVKVNWATTPSSQKKDTSNHFHVFVGDLSPEITTEDIKSAFAPFGKISDARVVKDMATGKSKGYGFVSFYNKLDAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQENT
IRESfinder result
IRESfinder score
0.606075767
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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