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mmu_circThada_432
Basic information of circular RNA
PanCircBase ID:
mmu_circThada_432
mm39_Chr. Position ID:
chr17|84733566|84736632|-
Gene name:
Thada
Transcript:
ENSMUST00000047524.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
20,19,18
Exon/intron offsets:
0,2547,2930
Genomic length:
3066
Exon/intron sizes in nt:
15,91,37,136
Flanking exon/introns:
chr17:84730617-84733566|chr17:84736632-84739606
Splice length:
432
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
TTATTAAATGCTTGATGGAAAACCTTGAGGATGAAATATCTCAAGCTGAGAACTCTCTGCTTCAGGCTGCGTCTTCATTTCCAATGTATGGGAGAGTCCACTGTATAACAAGAGCTTTTCAGAGGTTGCCTCTAAATGACCTGCGGCTGGCGAGCGAGTGGAGGCCGCTGCTGGGGAGGCTTCTGCTGCTGTCCTACAGGCTTTCCACTGTGGTGGCCCCTGTCATCCAGAGCTCCTCTCCTGAAGGCCTCATCCCTGTGGACACTGACTCAGCGTCAGCAAGCCGCTTGCAGCTCATTCTCAATGAGATCCAGCCACGGGATACTAACGATTACTTCAACCACACCAAAATACTGAAAGAATGTGATAGCTTTGATTTGGAGGACTTGAGTACCAGTGTCTCAAATATTGATAGTTCTGCAGAAGTTAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTTTGATTTGGAGGACTTGAGT
59.18
TGGAAATGAAGACGCAGCCT
59.96
146
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGATTTGGAGGACTTGAGTACCA
59.09
TGGAAATGAAGACGCAGCCT
59.96
142
PCR template sequence:
TACTTCAACCACACCAAAATACTGAAAGAATGTGATAGCTTTGATTTGGAGGACTTGAGTACCAGTGTCTCAAATATTGATAGTTCTGCAGAAGTTAAAGTTATTAAATGCTTGATGGAAAACCTTGAGGATGAAATATCTCAAGCTGAGAACTCTCTGCTTCAGGCTGCGTCTTCATTTCCAATGTATGGGAGAGTCCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-544-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCAGAAGTTAAAGTTATTAAATGCTT
siRNA sets
TTTAATAACTTTAACTTCT, TAATAACTTTAACTTCTGC, ATTTAATAACTTTAACTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTHADA_432_ORF_1
1.0949
0.19972736
0.976248704
849
ORF sequence:
ATGGAAAACCTTGAGGATGAAATATCTCAAGCTGAGAACTCTCTGCTTCAGGCTGCGTCTTCATTTCCAATGTATGGGAGAGTCCACTGTATAACAAGAGCTTTTCAGAGGTTGCCTCTAAATGACCTGCGGCTGGCGAGCGAGTGGAGGCCGCTGCTGGGGAGGCTTCTGCTGCTGTCCTACAGGCTTTCCACTGTGGTGGCCCCTGTCATCCAGAGCTCCTCTCCTGAAGGCCTCATCCCTGTGGACACTGACTCAGCGTCAGCAAGCCGCTTGCAGCTCATTCTCAATGAGATCCAGCCACGGGATACTAACGATTACTTCAACCACACCAAAATACTGAAAGAATGTGATAGCTTTGATTTGGAGGACTTGAGTACCAGTGTCTCAAATATTGATAGTTCTGCAGAAGTTAAAGTTATTAAATGCTTGATGGAAAACCTTGAGGATGAAATATCTCAAGCTGAGAACTCTCTGCTTCAGGCTGCGTCTTCATTTCCAATGTATGGGAGAGTCCACTGTATAACAAGAGCTTTTCAGAGGTTGCCTCTAAATGACCTGCGGCTGGCGAGCGAGTGGAGGCCGCTGCTGGGGAGGCTTCTGCTGCTGTCCTACAGGCTTTCCACTGTGGTGGCCCCTGTCATCCAGAGCTCCTCTCCTGAAGGCCTCATCCCTGTGGACACTGACTCAGCGTCAGCAAGCCGCTTGCAGCTCATTCTCAATGAGATCCAGCCACGGGATACTAACGATTACTTCAACCACACCAAAATACTGAAAGAATGTGATAGCTTTGATTTGGAGGACTTGAGTACCAGTGTCTCAAATATTGATAGTTCTGCAGAAGTTAAA
Peptide sequence:
MENLEDEISQAENSLLQAASSFPMYGRVHCITRAFQRLPLNDLRLASEWRPLLGRLLLLSYRLSTVVAPVIQSSSPEGLIPVDTDSASASRLQLILNEIQPRDTNDYFNHTKILKECDSFDLEDLSTSVSNIDSSAEVKVIKCLMENLEDEISQAENSLLQAASSFPMYGRVHCITRAFQRLPLNDLRLASEWRPLLGRLLLLSYRLSTVVAPVIQSSSPEGLIPVDTDSASASRLQLILNEIQPRDTNDYFNHTKILKECDSFDLEDLSTSVSNIDSSAEVK
IRESfinder result
IRESfinder score
0.707171614
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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