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mmu_circTcirg1_389
Basic information of circular RNA
PanCircBase ID:
mmu_circTcirg1_389
mm39_Chr. Position ID:
chr19|3953339|3954295|-
Gene name:
Tcirg1
Transcript:
ENSMUST00000145791.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,169,877
Genomic length:
956
Exon/intron sizes in nt:
89,221,79
Flanking exon/introns:
chr19:3953172-3953339|chr19:3954295-3954584
Splice length:
389
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
CTCAACGAATCCGTGAGCGCCTTCCAGAGACGCTTCGTGGTAGACGTCCGGCGCTGCGAGGAACTGGAGAAGACGTTTACCTTCTTGCGGGAAGAAGTGCAGCGGGCAGGTCTGACGCTGGCCCCACCTGAGGGGACACTGCCAGCACCACCTCCCCGAGACCTGCTGCGCATCCAGGAGGAAACGGATCGCTTGGCCCAGGAGCTTCGGGATGTTCGTGGCAATCAGCAGGCACTGCGGGCCCAGCTGCACCAGCTGCGGCTTCACTCGGCTGTGCTGGGCCAGAGCCACAGCCCGCCGGTGGCAGCTGACCATACTGAAGGACCCTTCTCTGAGACAACACCCCTGCTTCCAGGCACCCGGGGGCCACATTCAGACCTGAAGGTCAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGACAACACCCCTGCTTC
59.89
TCCCGCAAGAAGGTAAACGT
59.61
149
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGAGACAACACCCCTGCTT
59.89
TCCCGCAAGAAGGTAAACGT
59.61
150
PCR template sequence:
ACAGCCCGCCGGTGGCAGCTGACCATACTGAAGGACCCTTCTCTGAGACAACACCCCTGCTTCCAGGCACCCGGGGGCCACATTCAGACCTGAAGGTCAACTCAACGAATCCGTGAGCGCCTTCCAGAGACGCTTCGTGGTAGACGTCCGGCGCTGCGAGGAACTGGAGAAGACGTTTACCTTCTTGCGGGAAGAAGTGC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-199a-5p(1), mmu-miR-34a-5p(1), mmu-miR-207(1)
siRNAs for circular RNA silencing
Junction sequence
ACCTGAAGGTCAACTCAACGAATCCG
siRNA sets
TTCGTTGAGTTGACCTTCA, TCGTTGAGTTGACCTTCAG, ATTCGTTGAGTTGACCTTC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.238076945
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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