top of page
mmu_circTcea1_762
Basic information of circular RNA
PanCircBase ID:
mmu_circTcea1_762
mm39_Chr. Position ID:
chr1|4937692|4963786|+
Gene name:
Tcea1
Transcript:
ENSMUST00000081551.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
7
Exon/intron indexes:
2,3,4,5,6,7,8
Exon/intron offsets:
0,10557,19274,21987,23270,24445,25947
Genomic length:
26094
Exon/intron sizes in nt:
6,31,06,88,14,65,71,55,000
Flanking exon/introns:
chr1:4928199-4937692|chr1:4963786-4965156
Splice length:
762
Other database IDs:
circBase: hsa_circ_0084522, CIRCpedia: HSA_CIRCpedia_61363
Human Ortholog IDs:
Mature spliced Sequence:
GCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTCAAAGAGATGAGGAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACTTATACACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GTGGGACCCAGACTGACTTG
59.96
GCGAAGGGCATTGACAGACA
60.96
164
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGACCCAGACTGACTTGT
60.4
GCGAAGGGCATTGACAGACA
60.96
163
PCR template sequence:
AGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACTTATACACAGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-143-3p(1), mmu-miR-188-3p(1), mmu-miR-670-5p(1), mmu-miR-3105-3p(1), mmu-miR-7a-2-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CACTTATACACAGGCTGGCGCATTGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTCEA1_762_ORF_1
1.0188
0.135278623
0.99961565
1482
ORF sequence:
ATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTCAAAGAGATGAGGAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACTTATACACAGGCTGGCGCATTGGATTTGCTGAAAGAGCTTAAGAATATTCCCATGACCCTAGAATTGCTACAGTCCACAAGAATTGGAATGTCTGTCAATGCCCTTCGCAAGCAGAGCACAGATGAAGAAGTCACCTCTCTAGCCAAGTCTCTCATCAAATCCTGGAAAAAATTATTAGATGGACCATCAACTGATAAAGACCCTGAAGAAAAGAAAAAAGAGCCTGCAATTTCATCACAGAATAGCCCTGAAGCAAGAGAAGAAAGTAGTTCCAGCAGCAATGTAAGCAGCAGAAAGGATGAGACAAATGCTCGAGATACATATGTTTCATCTTTTCCTCGGGCACCAAGCACTTCTGATTCTGTGCGATTAAAATGTAGGGAGATGCTTGCTGCAGCTCTTCGGACAGGAGATGATTATGTTGCAATTGGAGCTGATGAAGAAGAACTGGGATCTCAGATTGAGGAAGCTATATATCAAGAAATAAGGAATACAGACATGAAATACAAAAACAGAGTACGAAGTAGGATATCAAATCTTAAAGATGCAAAGAATCCAAATTTAAGGAAAAATGTGCTGTGTGGGAATATTCCTCCTGATCTATTTGCTAGAATGACAGCAGAGGAAATGGCTAGTGATGAGCTCAAAGAGATGAGGAAAAACCTGACCAAAGAAGCCATCAGGGAGCATCAGATGGCCAAGACTGGTGGGACCCAGACTGACTTGTTCACTTGTGGCAAATGTAAAAAGAAGAACTGCACTTATACACAG
Peptide sequence:
MTLELLQSTRIGMSVNALRKQSTDEEVTSLAKSLIKSWKKLLDGPSTDKDPEEKKKEPAISSQNSPEAREESSSSSNVSSRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYVAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQAGALDLLKELKNIPMTLELLQSTRIGMSVNALRKQSTDEEVTSLAKSLIKSWKKLLDGPSTDKDPEEKKKEPAISSQNSPEAREESSSSSNVSSRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYVAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQ
IRESfinder result
IRESfinder score
0.443938741
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
bottom of page