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mmu_circTbp_443
Basic information of circular RNA
PanCircBase ID:
mmu_circTbp_443
mm39_Chr. Position ID:
chr17|15727604|15735972|+
Gene name:
Tbp
Transcript:
ENSMUST00000014911.12
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,6,7,8
Exon/intron offsets:
0,6187,6887,8273
Genomic length:
8368
Exon/intron sizes in nt:
88,92,168,95
Flanking exon/introns:
chr17:15724909-15727604|chr17:15735972-15736965
Splice length:
443
Other database IDs:
circAtlas: mmu-Tbp_0001
Human Ortholog IDs:
Mature spliced Sequence:
AAATATTGTATCTACCGTGAATCTTGGCTGTAAACTTGACCTAAAGACCATTGCACTTCGTGCAAGAAATGCTGAATATAATCCCAAGCGATTTGCTGCAGTCATCATGAGAATAAGAGAGCCACGGACAACTGCGTTGATTTTCAGTTCTGGAAAAATGGTGTGCACAGGAGCCAAGAGTGAAGAACAATCCAGACTAGCAGCAAGAAAATATGCTAGAGTTGTGCAGAAGTTGGGCTTCCCAGCTAAGTTCTTAGACTTCAAGATCCAGAACATGGTGGGGAGCTGTGATGTGAAGTTCCCTATAAGGCTGGAAGGCCTTGTGCTGACCCACCAGCAGTTCAGTAGCTATGAGCCAGAATTATTTCCTGGATTAATCTACAGAATGATCAAACCCAGAATTGTTCTCCTTATTTTTGTTTCTGGAAAAGTTGTATTAACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGCCAGAATTATTTCCTGGA
57.14
CGAAGTGCAATGGTCTTTAGGT
58.93
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGAGCCAGAATTATTTCCTGGA
57.14
ACGAAGTGCAATGGTCTTTAGG
58.93
153
PCR template sequence:
AGTAGCTATGAGCCAGAATTATTTCCTGGATTAATCTACAGAATGATCAAACCCAGAATTGTTCTCCTTATTTTTGTTTCTGGAAAAGTTGTATTAACAGAAATATTGTATCTACCGTGAATCTTGGCTGTAAACTTGACCTAAAGACCATTGCACTTCGTGCAAGAAATGCTGAATATAATCCCAAGCGATTTGCTGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
6
Interacting miRNAs (#binding sites)
mmu-miR-130a-3p(1), mmu-miR-138-5p(1), mmu-miR-130b-3p(1), mmu-miR-301b-3p(1), mmu-miR-301a-3p(1), mmu-miR-673-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GTTGTATTAACAGAAATATTGTATCT
siRNA sets
CAATATTTCTGTTAATACA, AATATTTCTGTTAATACAA, ACAATATTTCTGTTAATAC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBP_443_ORF_1
0.796
-0.028438104
0.210220054
357
ORF sequence:
ATGAGAATAAGAGAGCCACGGACAACTGCGTTGATTTTCAGTTCTGGAAAAATGGTGTGCACAGGAGCCAAGAGTGAAGAACAATCCAGACTAGCAGCAAGAAAATATGCTAGAGTTGTGCAGAAGTTGGGCTTCCCAGCTAAGTTCTTAGACTTCAAGATCCAGAACATGGTGGGGAGCTGTGATGTGAAGTTCCCTATAAGGCTGGAAGGCCTTGTGCTGACCCACCAGCAGTTCAGTAGCTATGAGCCAGAATTATTTCCTGGATTAATCTACAGAATGATCAAACCCAGAATTGTTCTCCTTATTTTTGTTTCTGGAAAAGTTGTATTAACAGAAATATTGTATCTACCGTGA
Peptide sequence:
MRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTEILYLP
IRESfinder result
IRESfinder score
0.652566999
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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