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mmu_circTbc1d5_131
Basic information of circular RNA
PanCircBase ID:
mmu_circTbc1d5_131
mm39_Chr. Position ID:
chr17|51332101|51332232|-
Gene name:
Tbc1d5
Transcript:
ENSMUST00000024717.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
3
Exon/intron offsets:
0
Genomic length:
131
Exon/intron sizes in nt:
131
Flanking exon/introns:
chr17:51291702-51332101|chr17:51332232-51450386
Splice length:
131
Other database IDs:
circBase: hsa_circ_0064553, CIRCpedia: HSA_CIRCpedia_349999
Human Ortholog IDs:
Mature spliced Sequence:
ACAATTACTTCGGTGACTGTCTCCACTCTATGCAATGTATAAGTCTGTGTCTGAAACGAGACATCCTCTGCAGTCAGAAGAACAAGAAGTAGGCATTGACCCCTTGTTCAGTTATTCAAATAAAACAAGAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
TCTGCAGTCAGAAGAACAAGA
56.9
TGTCTCGTTTCAGACACAGACT
59.31
128
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGCAGTCAGAAGAACAAGAAGT
58.87
TGTCTCGTTTCAGACACAGACT
59.31
127
PCR template sequence:
TCTGCAGTCAGAAGAACAAGAAGTAGGCATTGACCCCTTGTTCAGTTATTCAAATAAAACAAGAGACAATTACTTCGGTGACTGTCTCCACTCTATGCAATGTATAAGTCTGTGTCTGAAACGAGACATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-298-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AATAAAACAAGAGACAATTACTTCGG
siRNA sets
TAATTGTCTCTTGTTTTAT, AATTGTCTCTTGTTTTATT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBC1D5_131_ORF_1
0.5462
0.018896684
0.05773646
189
ORF sequence:
ATGTATAAGTCTGTGTCTGAAACGAGACATCCTCTGCAGTCAGAAGAACAAGAAGTAGGCATTGACCCCTTGTTCAGTTATTCAAATAAAACAAGAGACAATTACTTCGGTGACTGTCTCCACTCTATGCAATGTATAAGTCTGTGTCTGAAACGAGACATCCTCTGCAGTCAGAAGAACAAGAAGTAG
Peptide sequence:
MYKSVSETRHPLQSEEQEVGIDPLFSYSNKTRDNYFGDCLHSMQCISLCLKRDILCSQKNKK
IRESfinder result
IRESfinder score
0.780621154
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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