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mmu_circTbc1d14_484
Basic information of circular RNA
PanCircBase ID:
mmu_circTbc1d14_484
mm39_Chr. Position ID:
chr5|36669797|36680353|-
Gene name:
Tbc1d14
Transcript:
ENSMUST00000146430.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
9,8,7,6,5
Exon/intron offsets:
0,1585,6819,7990,10473
Genomic length:
10556
Exon/intron sizes in nt:
95,81,107,118,83
Flanking exon/introns:
chr5:36665775-36669797|chr5:36680353-36682273
Splice length:
484
Other database IDs:
circAtlas: mmu-Tbc1d14_0034
circBase: hsa_circ_0069112, circAtlas: hsa-TBC1D14_0024, CIRCpedia: HSA_CIRCpedia_147405
Human Ortholog IDs:
Mature spliced Sequence:
AAATCTCCCAGCCAAGCCAGCTGAAGAAGCTCAAAAGCACAGACAGCAGTATGAAGAGATGGTGCTGCAGGCCAAGAAACGAGAGCTTAAAGAAGCCCAGCGTCGGAGGAAGCAGCTGGAGGAAAGGTGCAAGGTTGAAGAGAGCATTGGGAACGCTGTCCTCACCTGGAACAATGAGATCTTGCCTAACTGGGAAACCATGTGGTGCTCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGTTGAACATCACTCACGAGCTCTTTGACATCTGTCTTGCTCGGGCCAAGGAGCGGTGGCGGTCCCTCAGCACTGGAGGCTCAGAAGTGGAGAATGAAGATGCTGGGTTTTCAGCAGCAGACAGAGAGGCCAGTCTGGAGCTTATTAAACTGGACATTTCTAGAACATTTCCTAATCTCTGCATTTTCCAGCAA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AGAGGCCAGTCTGGAGCTTA
59.96
CCTGCAGCACCATCTCTTCA
60.04
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TGGGTTTTCAGCAGCAGACA
60.11
CCTGCAGCACCATCTCTTCA
60.04
162
PCR template sequence:
TGAAGATGCTGGGTTTTCAGCAGCAGACAGAGAGGCCAGTCTGGAGCTTATTAAACTGGACATTTCTAGAACATTTCCTAATCTCTGCATTTTCCAGCAAAAATCTCCCAGCCAAGCCAGCTGAAGAAGCTCAAAAGCACAGACAGCAGTATGAAGAGATGGTGCTGCAGGCCAAGAAACGAGAGCTTAAAGAAGCCCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-29b-3p(2), mmu-miR-31-5p(1), mmu-miR-29a-3p(1), mmu-miR-29c-3p(1), mmu-miR-541-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-335-3p(1), mmu-miR-338-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CATTTTCCAGCAAAAATCTCCCAGCC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBC1D14_484_ORF_1
0.8386
0.222818945
0.806324084
585
ORF sequence:
ATGAAGATGCTGGGTTTTCAGCAGCAGACAGAGAGGCCAGTCTGGAGCTTATTAAACTGGACATTTCTAGAACATTTCCTAATCTCTGCATTTTCCAGCAAAAATCTCCCAGCCAAGCCAGCTGAAGAAGCTCAAAAGCACAGACAGCAGTATGAAGAGATGGTGCTGCAGGCCAAGAAACGAGAGCTTAAAGAAGCCCAGCGTCGGAGGAAGCAGCTGGAGGAAAGGTGCAAGGTTGAAGAGAGCATTGGGAACGCTGTCCTCACCTGGAACAATGAGATCTTGCCTAACTGGGAAACCATGTGGTGCTCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGTTGAACATCACTCACGAGCTCTTTGACATCTGTCTTGCTCGGGCCAAGGAGCGGTGGCGGTCCCTCAGCACTGGAGGCTCAGAAGTGGAGAATGAAGATGCTGGGTTTTCAGCAGCAGACAGAGAGGCCAGTCTGGAGCTTATTAAACTGGACATTTCTAGAACATTTCCTAATCTCTGCATTTTCCAGCAA
Peptide sequence:
MKMLGFQQQTERPVWSLLNWTFLEHFLISAFSSKNLPAKPAEEAQKHRQQYEEMVLQAKKRELKEAQRRRKQLEERCKVEESIGNAVLTWNNEILPNWETMWCSKKVRDLWWQGIPPSVRGKVWSLAIGNELNITHELFDICLARAKERWRSLSTGGSEVENEDAGFSAADREASLELIKLDISRTFPNLCIFQQ
IRESfinder result
IRESfinder score
0.442932131
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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