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mmu_circTbc1d14_311
Basic information of circular RNA
PanCircBase ID:
mmu_circTbc1d14_311
mm39_Chr. Position ID:
chr5|36662368|36665775|-
Gene name:
Tbc1d14
Transcript:
ENSMUST00000146430.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
12,11,10
Exon/intron offsets:
0,2553,3335
Genomic length:
3407
Exon/intron sizes in nt:
110,129,72
Flanking exon/introns:
chr5:36652620-36662368|chr5:36665775-36669797
Splice length:
311
Other database IDs:
CIRCpedia: MMU_CIRCpedia_18112, circAtlas: mmu-Tbc1d14_0012
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_117722
Human Ortholog IDs:
Mature spliced Sequence:
GGTGGTCCGTATCATGACATGTTGCACAGTATTTTGGGCGCTTATACTTGTTACCGGCCGGATGTGGGTTATGTTCAGGGCATGTCCTTTATAGCTGCAGTGTTGATCTTGAACCTCGATACTGCAGATGCCTTTATTGCTTTTTCTAATCTTTTGAATAAACCCTGTCAAATGGCGTTTTTCCGAGTGGACCATGGCCTTATGTTGACTTACTTTGCTGCATTTGAGGTATTCTTTGAAGAAAACTTGCCGAAATTGTTTGCGCATTTCAAGAAAAACAACTTAACTGCAGATATCTACCTAATCGATTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTTGCCGAAATTGTTTGCGC
59.84
AACATAACCCACATCCGGCC
60.4
141
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
TTGCCGAAATTGTTTGCGCA
60.53
AACATAACCCACATCCGGCC
60.4
140
PCR template sequence:
ACTTTGCTGCATTTGAGGTATTCTTTGAAGAAAACTTGCCGAAATTGTTTGCGCATTTCAAGAAAAACAACTTAACTGCAGATATCTACCTAATCGATTGGGTGGTCCGTATCATGACATGTTGCACAGTATTTTGGGCGCTTATACTTGTTACCGGCCGGATGTGGGTTATGTTCAGGGCATGTCCTTTATAGCTGCAG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-200c-3p(1), mmu-miR-203-3p(1), mmu-miR-382-5p(1), mmu-miR-340-5p(1), mmu-miR-680(1)
siRNAs for circular RNA silencing
Junction sequence
ACCTAATCGATTGGGTGGTCCGTATC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBC1D14_311_ORF_1
0.9634
-0.062355709
0.283270874
387
ORF sequence:
ATGTTGCACAGTATTTTGGGCGCTTATACTTGTTACCGGCCGGATGTGGGTTATGTTCAGGGCATGTCCTTTATAGCTGCAGTGTTGATCTTGAACCTCGATACTGCAGATGCCTTTATTGCTTTTTCTAATCTTTTGAATAAACCCTGTCAAATGGCGTTTTTCCGAGTGGACCATGGCCTTATGTTGACTTACTTTGCTGCATTTGAGGTATTCTTTGAAGAAAACTTGCCGAAATTGTTTGCGCATTTCAAGAAAAACAACTTAACTGCAGATATCTACCTAATCGATTGGGTGGTCCGTATCATGACATGTTGCACAGTATTTTGGGCGCTTATACTTGTTACCGGCCGGATGTGGGTTATGTTCAGGGCATGTCCTTTATAG
Peptide sequence:
MLHSILGAYTCYRPDVGYVQGMSFIAAVLILNLDTADAFIAFSNLLNKPCQMAFFRVDHGLMLTYFAAFEVFFEENLPKLFAHFKKNNLTADIYLIDWVVRIMTCCTVFWALILVTGRMWVMFRACPL
IRESfinder result
IRESfinder score
0.739890686
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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