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mmu_circTbc1d14_225
Basic information of circular RNA
PanCircBase ID:
mmu_circTbc1d14_225
mm39_Chr. Position ID:
chr5|36676616|36677905|-
Gene name:
Tbc1d14
Transcript:
ENSMUST00000146430.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
7,6
Exon/intron offsets:
0,1171
Genomic length:
1289
Exon/intron sizes in nt:
1,07,118
Flanking exon/introns:
chr5:36671463-36676616|chr5:36677905-36680270
Splice length:
225
Other database IDs:
circAtlas: mmu-Tbc1d14_0030
circBase: hsa_circ_0069113, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_49004
Human Ortholog IDs:
Mature spliced Sequence:
AGCTTAAAGAAGCCCAGCGTCGGAGGAAGCAGCTGGAGGAAAGGTGCAAGGTTGAAGAGAGCATTGGGAACGCTGTCCTCACCTGGAACAATGAGATCTTGCCTAACTGGGAAACCATGTGGTGCTCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGTTGAACATCACTCACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTTGTGGTGGCAGGGAATC
60.25
TTGTTCCAGGTGAGGACAGC
59.89
175
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GACTTGTGGTGGCAGGGAAT
60.25
TTGTTCCAGGTGAGGACAGC
59.89
176
PCR template sequence:
TCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGTTGAACATCACTCACGAGCTTAAAGAAGCCCAGCGTCGGAGGAAGCAGCTGGAGGAAAGGTGCAAGGTTGAAGAGAGCATTGGGAACGCTGTCCTCACCTGGAACAATGAGATCTT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
4
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-29b-3p(1), mmu-miR-541-5p(1), mmu-miR-29b-2-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AACATCACTCACGAGCTTAAAGAAGC
siRNA sets
TTCTTTAAGCTCGTGAGTG, TTTAAGCTCGTGAGTGATG, TTAAGCTCGTGAGTGATGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTBC1D14_225_ORF_1
0.8108
0.239255783
0.409843756
333
ORF sequence:
ATGTGGTGCTCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGTTGAACATCACTCACGAGCTTAAAGAAGCCCAGCGTCGGAGGAAGCAGCTGGAGGAAAGGTGCAAGGTTGAAGAGAGCATTGGGAACGCTGTCCTCACCTGGAACAATGAGATCTTGCCTAACTGGGAAACCATGTGGTGCTCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGAGGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGTTGAACATCACTCAC
Peptide sequence:
MWCSKKVRDLWWQGIPPSVRGKVWSLAIGNELNITHELKEAQRRRKQLEERCKVEESIGNAVLTWNNEILPNWETMWCSKKVRDLWWQGIPPSVRGKVWSLAIGNELNITH
IRESfinder result
IRESfinder score
0.320344446
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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