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mmu_circTanc2_815
Basic information of circular RNA
PanCircBase ID:
mmu_circTanc2_815
mm39_Chr. Position ID:
chr11|105725774|105754909|+
Gene name:
Tanc2
Transcript:
ENSMUST00000100330.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
9,10,11,12
Exon/intron offsets:
0,5670,22448,28968
Genomic length:
29135
Exon/intron sizes in nt:
2,82,13,42,32,167
Flanking exon/introns:
chr11:105701423-105725774|chr11:105754909-105757992
Splice length:
815
Other database IDs:
circAtlas: mmu-Tanc2_0027
circAtlas: hsa-SCAF8_0001
Human Ortholog IDs:
Mature spliced Sequence:
TTCGCTTTGCACCTTACAGACCTCCAGACATCTCCTTGAAGCCCCTACTCTTCGAAGTGCCCAGCATTACCACGGAGTCAGTGTTTGTTGGTCGAGATTGGGTATTCCATGAGATTGATGCTCAGCTTCAGAGTTCTAATGCCAGCGTGAACCAAGGAGTAGTGATTGTGGGAAACATTGGGTTCGGCAAAACTGCCATCATCTCCAGACTGGTGGCCCTCAGCTGCCATGGTACACGGATGAGACAGATCGCCTCTGACAGCCCACACGCCTCCCCCAAACATGTGGACGCCAACAGAGAGCTACCACTCACCCAAGCACCTTCAGCTCACTCATCTATTACCAGTGGAAGCTGCCCAGGAACTCCAGAAATGCGCAGGCGGCAAGAGGAAGCCATGCGAAGACTTGCCTCACAGGTGGTTGCCTATCACTATTGCCAGGCAGATAATGCCTACACCTGCCTGGTACCAGAATTCGTCCACAATGTTGCTGCCCTGCTCTGCCGCTCACCTCAGCTGACAGCTTACCGGGAACAGCTTCTTCGGGAGCCTCACCTGCAGAGCATGCTAAGCCTCCGGTCCTGTGTTCAAGATCCCATGGCCTCCTTCCGGAGAGGAGTCCTAGAGCCCTTGGAGAACCTCCACAAAGAGAGAAAAATCCCAGATGAAGATTTCATCATTTTAATTGATGGATTAAATGAAGCAGAATTTCACAAACCGGATTATGGGGATACAATTGTATCATTTCTAAGTAAAATGATTGGAAATTTTCCTTCTTGGCTCAAACTAATTGTAACAGTTAGGACCAGTTTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCGGATTATGGGGATACAATTGT
59.89
AATGCTGGGCACTTCGAAGA
59.96
168
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCGGATTATGGGGATACAATTGT
59.89
GGCACTTCGAAGAGTAGGGG
59.82
161
PCR template sequence:
ACCGGATTATGGGGATACAATTGTATCATTTCTAAGTAAAATGATTGGAAATTTTCCTTCTTGGCTCAAACTAATTGTAACAGTTAGGACCAGTTTACAGTTCGCTTTGCACCTTACAGACCTCCAGACATCTCCTTGAAGCCCCTACTCTTCGAAGTGCCCAGCATTACCACGGAGTCAGTGTTTGTTGGTCGAGATTG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-200a-3p(1), mmu-miR-141-3p(1), mmu-miR-429-3p(1), mmu-miR-200b-3p(1), mmu-miR-135a-5p(1), mmu-miR-200c-3p(1), mmu-miR-143-3p(1), mmu-miR-495-3p(1), mmu-miR-127-5p(1), mmu-miR-382-5p(1), mmu-miR-27a-5p(1), mmu-miR-3065-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACCAGTTTACAGTTCGCTTTGCACC
siRNA sets
AAGCGAACTGTAAACTGGT, AAAGCGAACTGTAAACTGG, CAAAGCGAACTGTAAACTG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTANC2_815_ORF_1
0.8018
0.153329695
0.781559285
615
ORF sequence:
ATGAGACAGATCGCCTCTGACAGCCCACACGCCTCCCCCAAACATGTGGACGCCAACAGAGAGCTACCACTCACCCAAGCACCTTCAGCTCACTCATCTATTACCAGTGGAAGCTGCCCAGGAACTCCAGAAATGCGCAGGCGGCAAGAGGAAGCCATGCGAAGACTTGCCTCACAGGTGGTTGCCTATCACTATTGCCAGGCAGATAATGCCTACACCTGCCTGGTACCAGAATTCGTCCACAATGTTGCTGCCCTGCTCTGCCGCTCACCTCAGCTGACAGCTTACCGGGAACAGCTTCTTCGGGAGCCTCACCTGCAGAGCATGCTAAGCCTCCGGTCCTGTGTTCAAGATCCCATGGCCTCCTTCCGGAGAGGAGTCCTAGAGCCCTTGGAGAACCTCCACAAAGAGAGAAAAATCCCAGATGAAGATTTCATCATTTTAATTGATGGATTAAATGAAGCAGAATTTCACAAACCGGATTATGGGGATACAATTGTATCATTTCTAAGTAAAATGATTGGAAATTTTCCTTCTTGGCTCAAACTAATTGTAACAGTTAGGACCAGTTTACAGTTCGCTTTGCACCTTACAGACCTCCAGACATCTCCTTGA
Peptide sequence:
MRQIASDSPHASPKHVDANRELPLTQAPSAHSSITSGSCPGTPEMRRRQEEAMRRLASQVVAYHYCQADNAYTCLVPEFVHNVAALLCRSPQLTAYREQLLREPHLQSMLSLRSCVQDPMASFRRGVLEPLENLHKERKIPDEDFIILIDGLNEAEFHKPDYGDTIVSFLSKMIGNFPSWLKLIVTVRTSLQFALHLTDLQTSP
IRESfinder result
IRESfinder score
0.601038343
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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