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mmu_circTanc2_533
Basic information of circular RNA
PanCircBase ID:
mmu_circTanc2_533
mm39_Chr. Position ID:
chr11|105731444|105754909|+
Gene name:
Tanc2
Transcript:
ENSMUST00000100330.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
10,11,12
Exon/intron offsets:
0,16778,23298
Genomic length:
23465
Exon/intron sizes in nt:
13,42,32,167
Flanking exon/introns:
chr11:105726056-105731444|chr11:105754909-105757992
Splice length:
533
Other database IDs:
Human Ortholog IDs:
Mature spliced Sequence:
ATGTGGACGCCAACAGAGAGCTACCACTCACCCAAGCACCTTCAGCTCACTCATCTATTACCAGTGGAAGCTGCCCAGGAACTCCAGAAATGCGCAGGCGGCAAGAGGAAGCCATGCGAAGACTTGCCTCACAGGTGGTTGCCTATCACTATTGCCAGGCAGATAATGCCTACACCTGCCTGGTACCAGAATTCGTCCACAATGTTGCTGCCCTGCTCTGCCGCTCACCTCAGCTGACAGCTTACCGGGAACAGCTTCTTCGGGAGCCTCACCTGCAGAGCATGCTAAGCCTCCGGTCCTGTGTTCAAGATCCCATGGCCTCCTTCCGGAGAGGAGTCCTAGAGCCCTTGGAGAACCTCCACAAAGAGAGAAAAATCCCAGATGAAGATTTCATCATTTTAATTGATGGATTAAATGAAGCAGAATTTCACAAACCGGATTATGGGGATACAATTGTATCATTTCTAAGTAAAATGATTGGAAATTTTCCTTCTTGGCTCAAACTAATTGTAACAGTTAGGACCAGTTTACAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACCGGATTATGGGGATACAATTGT
59.89
GGGCAGCTTCCACTGGTAAT
60.03
176
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ACCGGATTATGGGGATACAATTGT
59.89
CTGAAGGTGCTTGGGTGAGT
59.89
145
PCR template sequence:
ACCGGATTATGGGGATACAATTGTATCATTTCTAAGTAAAATGATTGGAAATTTTCCTTCTTGGCTCAAACTAATTGTAACAGTTAGGACCAGTTTACAGATGTGGACGCCAACAGAGAGCTACCACTCACCCAAGCACCTTCAGCTCACTCATCTATTACCAGTGGAAGCTGCCCAGGAACTCCAGAAATGCGCAGGCG
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-135a-5p(1), mmu-miR-382-5p(1), mmu-miR-27a-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GACCAGTTTACAGATGTGGACGCCAA
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTANC2_533_ORF_1
0.6518
0.143422676
0.809191754
681
ORF sequence:
ATGCGCAGGCGGCAAGAGGAAGCCATGCGAAGACTTGCCTCACAGGTGGTTGCCTATCACTATTGCCAGGCAGATAATGCCTACACCTGCCTGGTACCAGAATTCGTCCACAATGTTGCTGCCCTGCTCTGCCGCTCACCTCAGCTGACAGCTTACCGGGAACAGCTTCTTCGGGAGCCTCACCTGCAGAGCATGCTAAGCCTCCGGTCCTGTGTTCAAGATCCCATGGCCTCCTTCCGGAGAGGAGTCCTAGAGCCCTTGGAGAACCTCCACAAAGAGAGAAAAATCCCAGATGAAGATTTCATCATTTTAATTGATGGATTAAATGAAGCAGAATTTCACAAACCGGATTATGGGGATACAATTGTATCATTTCTAAGTAAAATGATTGGAAATTTTCCTTCTTGGCTCAAACTAATTGTAACAGTTAGGACCAGTTTACAGATGTGGACGCCAACAGAGAGCTACCACTCACCCAAGCACCTTCAGCTCACTCATCTATTACCAGTGGAAGCTGCCCAGGAACTCCAGAAATGCGCAGGCGGCAAGAGGAAGCCATGCGAAGACTTGCCTCACAGGTGGTTGCCTATCACTATTGCCAGGCAGATAATGCCTACACCTGCCTGGTACCAGAATTCGTCCACAATGTTGCTGCCCTGCTCTGCCGCTCACCTCAGCTGA
Peptide sequence:
MRRRQEEAMRRLASQVVAYHYCQADNAYTCLVPEFVHNVAALLCRSPQLTAYREQLLREPHLQSMLSLRSCVQDPMASFRRGVLEPLENLHKERKIPDEDFIILIDGLNEAEFHKPDYGDTIVSFLSKMIGNFPSWLKLIVTVRTSLQMWTPTESYHSPKHLQLTHLLPVEAAQELQKCAGGKRKPCEDLPHRWLPITIARQIMPTPAWYQNSSTMLLPCSAAHLS
IRESfinder result
IRESfinder score
0.645312514
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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