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mmu_circTanc2_264
Basic information of circular RNA
PanCircBase ID:
mmu_circTanc2_264
mm39_Chr. Position ID:
chr11|105689386|105689650|+
Gene name:
Tanc2
Transcript:
ENSMUST00000100330.10
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
7
Exon/intron offsets:
0
Genomic length:
264
Exon/intron sizes in nt:
264
Flanking exon/introns:
chr11:105670951-105689386|chr11:105689650-105701297
Splice length:
264
Other database IDs:
CIRCpedia: MMU_CIRCpedia_1567, circAtlas: mmu-Tanc2_0045
circAtlas: hsa-TANC2_0047
Human Ortholog IDs:
Mature spliced Sequence:
CAACATTAACAAATTATTCTGAAAACATGGAGCGAACAAAATATGTTGGAGAAGGCAGTAAAGAATTAGGATCTGGAGGAAACCTAAAACCATGGCAGTCTCAAAAATCCAGCATGGACTCTTGTCTGTACCGAGTGGATGAAAACATGGCTGCATCCACATACAGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CGGAGCGAAATCTGGAAACA
58.56
GCCATGGTTTTAGGTTTCCTCC
59.5
180
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GAACAAGATCCCGGAGCGAA
60.11
TCCAACATATTTTGTTCGCTCCA
58.92
145
PCR template sequence:
AGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCAGCAACATTAACAAATTATTCTGAAAACATGGAGCGAACAAAATATGTTGGAGAAGGCAGTAAAGAATTAGGATCTGGAGGAAACCTAAAACCATGGCAGTC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-375-3p(1), mmu-miR-297a-5p(1), mmu-miR-335-3p(1)
siRNAs for circular RNA silencing
Junction sequence
AATTCTGTAGCAGCAACATTAACAAA
siRNA sets
GTTAATGTTGCTGCTACAG, TTGTTAATGTTGCTGCTAC, TTAATGTTGCTGCTACAGA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTANC2_264_ORF_1
0.7069
0.113785693
0.534867769
501
ORF sequence:
ATGGAGCGAACAAAATATGTTGGAGAAGGCAGTAAAGAATTAGGATCTGGAGGAAACCTAAAACCATGGCAGTCTCAAAAATCCAGCATGGACTCTTGTCTGTACCGAGTGGATGAAAACATGGCTGCATCCACATACAGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCAGCAACATTAACAAATTATTCTGAAAACATGGAGCGAACAAAATATGTTGGAGAAGGCAGTAAAGAATTAGGATCTGGAGGAAACCTAAAACCATGGCAGTCTCAAAAATCCAGCATGGACTCTTGTCTGTACCGAGTGGATGAAAACATGGCTGCATCCACATACAGTCTGAACAAGATCCCGGAGCGAAATCTGGAAACAGTTTTATCGCAATCAGTACAGTCTATTCCGTTGTATCTCATGCCTCGGCCAAATTCTGTAGCA
Peptide sequence:
MERTKYVGEGSKELGSGGNLKPWQSQKSSMDSCLYRVDENMAASTYSLNKIPERNLETVLSQSVQSIPLYLMPRPNSVAATLTNYSENMERTKYVGEGSKELGSGGNLKPWQSQKSSMDSCLYRVDENMAASTYSLNKIPERNLETVLSQSVQSIPLYLMPRPNSVA
IRESfinder result
IRESfinder score
0.411839012
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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