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mmu_circTaf5_737
Basic information of circular RNA
PanCircBase ID:
mmu_circTaf5_737
mm39_Chr. Position ID:
chr19|47063251|47065833|+
Gene name:
Taf5
Transcript:
ENSMUST00000026027.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
3,4,5,6
Exon/intron offsets:
0,923,1184,2461
Genomic length:
2582
Exon/intron sizes in nt:
3,16,16,41,36,121
Flanking exon/introns:
chr19:47059657-47063251|chr19:47065833-47067206
Splice length:
737
Other database IDs:
CIRCpedia: MMU_CIRCpedia_21030, circAtlas: mmu-Taf5_0001
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_76985
Human Ortholog IDs:
Mature spliced Sequence:
GTTCCATGGAGATCAGGAATGTTACTACCAGGATGACCTACGAGTGTTATCTAGTCTTACCAAAAAGGAGCACATGAAAGGGAACGAGACCATGCTGGACTTTCGGACGAGTAAATTCGTTCTCCGCATTTCCCGGGACTCGTACCAGCTCTTGAAGCGGCATCTCCAGGAGAAGCAGAACAATCAGATCTGGAACATCGTGCAGGAGCACCTCTACATCGACATCTTCGACGGGATGCCTCGCAGTAAGCAACAGATCGACGCCATGGTGGGCAGCCTGGCAGGAGAGGCGAAACGAGAGGCGAACAAATCGAAGGTATTTTTTGGTTTACTAAAAGAACCAGAAATTGAGGTGCCTTTGGATGATGAGGACGAAGAAGGAGAAAATGAAGAAGGAAAACCTAAAAAGAAGAAGCCTAAGAAGGATAGTATTGGCTCTAAAAGCAAAAAACAAGATCCCAATGCCCCGCCTCAAAACAGAATTCCTCTTCCTGAGTTAAAAGATTCAGACAAACTGGACAAAATAATGAATATGAAAGAAACTACCAAACGAGTGCGCCTCGGGCCAGACTGTTTACCCTCCATTTGTTTCTATACATTCCTTAATGCTTACCAGGGCCTCACTGCAGTGGATGTCACTGATGACTCTAGTCTGATTGCTGGAGGTTTTGCTGATTCAACTGTCAGAGTGTGGTCTGTGACCCCTAAAAAGCTTCGTAGTGTCAAACAAGCATCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
ACTGTCAGAGTGTGGTCTGTG
59.59
CAGCATGGTCTCGTTCCCTT
60.04
155
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CTGTCAGAGTGTGGTCTGTGA
59.32
CAGCATGGTCTCGTTCCCTT
60.04
154
PCR template sequence:
ACTGATGACTCTAGTCTGATTGCTGGAGGTTTTGCTGATTCAACTGTCAGAGTGTGGTCTGTGACCCCTAAAAAGCTTCGTAGTGTCAAACAAGCATCAGGTTCCATGGAGATCAGGAATGTTACTACCAGGATGACCTACGAGTGTTATCTAGTCTTACCAAAAAGGAGCACATGAAAGGGAACGAGACCATGCTGGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
12
Interacting miRNAs (#binding sites)
mmu-miR-1a-3p(1), mmu-miR-375-3p(1), mmu-miR-206-3p(1), mmu-miR-30e-5p(1), mmu-miR-211-5p(1), mmu-miR-30a-5p(1), mmu-miR-324-5p(1), mmu-miR-181b-5p(1), mmu-miR-96-5p(1), mmu-miR-129-5p(1), mmu-miR-207(1), mmu-miR-1904(1)
siRNAs for circular RNA silencing
Junction sequence
AAACAAGCATCAGGTTCCATGGAGAT
siRNA sets
CTCCATGGAACCTGATGCT, TCTCCATGGAACCTGATGC, CATGGAACCTGATGCTTGT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTAF5_737_ORF_1
0.9574
0.273349631
0.951096014
741
ORF sequence:
ATGAAAGGGAACGAGACCATGCTGGACTTTCGGACGAGTAAATTCGTTCTCCGCATTTCCCGGGACTCGTACCAGCTCTTGAAGCGGCATCTCCAGGAGAAGCAGAACAATCAGATCTGGAACATCGTGCAGGAGCACCTCTACATCGACATCTTCGACGGGATGCCTCGCAGTAAGCAACAGATCGACGCCATGGTGGGCAGCCTGGCAGGAGAGGCGAAACGAGAGGCGAACAAATCGAAGGTATTTTTTGGTTTACTAAAAGAACCAGAAATTGAGGTGCCTTTGGATGATGAGGACGAAGAAGGAGAAAATGAAGAAGGAAAACCTAAAAAGAAGAAGCCTAAGAAGGATAGTATTGGCTCTAAAAGCAAAAAACAAGATCCCAATGCCCCGCCTCAAAACAGAATTCCTCTTCCTGAGTTAAAAGATTCAGACAAACTGGACAAAATAATGAATATGAAAGAAACTACCAAACGAGTGCGCCTCGGGCCAGACTGTTTACCCTCCATTTGTTTCTATACATTCCTTAATGCTTACCAGGGCCTCACTGCAGTGGATGTCACTGATGACTCTAGTCTGATTGCTGGAGGTTTTGCTGATTCAACTGTCAGAGTGTGGTCTGTGACCCCTAAAAAGCTTCGTAGTGTCAAACAAGCATCAGGTTCCATGGAGATCAGGAATGTTACTACCAGGATGACCTACGAGTGTTATCTAGTCTTACCAAAAAGGAGCACATGA
Peptide sequence:
MKGNETMLDFRTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQEHLYIDIFDGMPRSKQQIDAMVGSLAGEAKREANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKPKKKKPKKDSIGSKSKKQDPNAPPQNRIPLPELKDSDKLDKIMNMKETTKRVRLGPDCLPSICFYTFLNAYQGLTAVDVTDDSSLIAGGFADSTVRVWSVTPKKLRSVKQASGSMEIRNVTTRMTYECYLVLPKRST
IRESfinder result
IRESfinder score
0.482565548
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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