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mmu_circTacc1_1293
Basic information of circular RNA
PanCircBase ID:
mmu_circTacc1_1293
mm39_Chr. Position ID:
chr8|25665157|25672946|-
Gene name:
Tacc1
Transcript:
ENSMUST00000084030.6
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
3
Exon/intron indexes:
5,4,3
Exon/intron offsets:
0,2792,6681
Genomic length:
7789
Exon/intron sizes in nt:
12,46,11,108
Flanking exon/introns:
chr8:25661340-25665157|chr8:25672946-25690106
Splice length:
1293
Other database IDs:
circAtlas: mmu-Tacc1_0046
Human Ortholog IDs:
Mature spliced Sequence:
AATCTCGAGAAGCCGATGAACAGTTGGTAGCAGAAGTAATTGAAAAATGTTCACCCGATACATGTTCTCGATCTTCAGAAAATGAAGCGCCACAGGCGACCGTCGATTCTCACCCAGTCAAGGATGTCAGAGGGAAGGCGGAACACGATGTTAGCAAGATCTCAGTAGTGAGGCCGTTTTCAATAGAAACCAGGAATTGTACAGATGACCCTGCGGCTCTGGGGACAGCAGCAGCCCATGGCTGTGTCCCCGTGCTCCCAGGCATGGCCTTGCCTTCCACCACACCGGAAGCCACTCAGGATGAGCCAGTGATGGACAGAGGCATGGGGGTTACATTGGAGGCTTTTACAGAAGCCAGTCTGAAGACTGGAGGACCCTGTCCAGAGCCCGTGGCCAGCAGAAGCAAGCTAAGAAAGCCCAAACCTGTCTCCCTGAGGAAGAAGATGGCCCCTGAACCGGAAATGTTGATGGAGGGTTCACCTCTCCCTAAGGCTTCCTCCCCATGGCTTCCTGATGGCTTGGATCAGAACGCGAATCCTAGTGTGCTGAGAGGCTCGGGGGCCCAGAGATCTCCCCTTAACCTTAAAGAAACAGCTGGTGTTCTCAGTAACGACACCAGTGACTCGGGGGTCGAGCTGCAGGGTGGGTCCAGGGACCCACCTCTCCAACTTGAGGATGATTTTACAGAAGATGGGGAGAATGTCAAGATCAGGAGCGCCCTTCCGAAGCAATCTGGCAGGAAACCCAGTAACAAACTGGCACCCAGTATACGAAAAGATGGCGTCAGTAAGCCAGTAGGTGTTGAGCAGCCTTCAGACCCAACAGTTCAAGATGCCTTGCTCGACCAGATGTCTCCTAAGCTAGATCCGAGTAAACGGAGTCATCCCCCCGCTAATTTCTTCGGGAGTGGCCCCATTCTGCAGAACTCCCCACCCCTCTCCTCCAAATGCTCCCACCACTTTGACCCAAATAACATTAACACTGATGACTCCGGGGATCCCTGCAAGCCAACCCCAGCTTTGACAAGCAGCGGCTTTTGTCCCGCCACTGGTAATCATGTTAATGAAATCTTAGACTCACCCAAGAAGGCAAAGTCACGTTTAATAACGAGCGGCTGCAAAGTGAAGAAATACGAAGCTCAGCCTCTCGATTTGGACGCGTGTTCCCAGGATGAAGGAGCCGTGATCTCGAAGATCTCAGAGATTCCCAACAGGGACGGCCACGCGACTGATGAGGAGAAGCTGGCCTCCACGTCGTCGTGTGCTCAGAAGTCAGCGGGGGCTGGGGTCAAAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GATGAGGAGAAGCTGGCCTC
59.89
GCCTGTGGCGCTTCATTTTC
60.73
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
CACGCGACTGATGAGGAGAA
59.83
GCCTGTGGCGCTTCATTTTC
60.73
170
PCR template sequence:
ATCTCAGAGATTCCCAACAGGGACGGCCACGCGACTGATGAGGAGAAGCTGGCCTCCACGTCGTCGTGTGCTCAGAAGTCAGCGGGGGCTGGGGTCAAAGAATCTCGAGAAGCCGATGAACAGTTGGTAGCAGAAGTAATTGAAAAATGTTCACCCGATACATGTTCTCGATCTTCAGAAAATGAAGCGCCACAGGCGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
7
Interacting miRNAs (#binding sites)
mmu-miR-211-5p(1), mmu-miR-204-5p(1), mmu-miR-320-3p(1), mmu-miR-326-3p(1), mmu-miR-138-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-362-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCTGGGGTCAAAGAATCTCGAGAAGC
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCTACC1_1293_ORF_1
1.0774
0.220610911
0.999999419
2322
ORF sequence:
ATGGCCTTGCCTTCCACCACACCGGAAGCCACTCAGGATGAGCCAGTGATGGACAGAGGCATGGGGGTTACATTGGAGGCTTTTACAGAAGCCAGTCTGAAGACTGGAGGACCCTGTCCAGAGCCCGTGGCCAGCAGAAGCAAGCTAAGAAAGCCCAAACCTGTCTCCCTGAGGAAGAAGATGGCCCCTGAACCGGAAATGTTGATGGAGGGTTCACCTCTCCCTAAGGCTTCCTCCCCATGGCTTCCTGATGGCTTGGATCAGAACGCGAATCCTAGTGTGCTGAGAGGCTCGGGGGCCCAGAGATCTCCCCTTAACCTTAAAGAAACAGCTGGTGTTCTCAGTAACGACACCAGTGACTCGGGGGTCGAGCTGCAGGGTGGGTCCAGGGACCCACCTCTCCAACTTGAGGATGATTTTACAGAAGATGGGGAGAATGTCAAGATCAGGAGCGCCCTTCCGAAGCAATCTGGCAGGAAACCCAGTAACAAACTGGCACCCAGTATACGAAAAGATGGCGTCAGTAAGCCAGTAGGTGTTGAGCAGCCTTCAGACCCAACAGTTCAAGATGCCTTGCTCGACCAGATGTCTCCTAAGCTAGATCCGAGTAAACGGAGTCATCCCCCCGCTAATTTCTTCGGGAGTGGCCCCATTCTGCAGAACTCCCCACCCCTCTCCTCCAAATGCTCCCACCACTTTGACCCAAATAACATTAACACTGATGACTCCGGGGATCCCTGCAAGCCAACCCCAGCTTTGACAAGCAGCGGCTTTTGTCCCGCCACTGGTAATCATGTTAATGAAATCTTAGACTCACCCAAGAAGGCAAAGTCACGTTTAATAACGAGCGGCTGCAAAGTGAAGAAATACGAAGCTCAGCCTCTCGATTTGGACGCGTGTTCCCAGGATGAAGGAGCCGTGATCTCGAAGATCTCAGAGATTCCCAACAGGGACGGCCACGCGACTGATGAGGAGAAGCTGGCCTCCACGTCGTCGTGTGCTCAGAAGTCAGCGGGGGCTGGGGTCAAAGAATCTCGAGAAGCCGATGAACAGTTGGTAGCAGAAGTAATTGAAAAATGTTCACCCGATACATGTTCTCGATCTTCAGAAAATGAAGCGCCACAGGCGACCGTCGATTCTCACCCAGTCAAGGATGTCAGAGGGAAGGCGGAACACGATGTTAGCAAGATCTCAGTAGTGAGGCCGTTTTCAATAGAAACCAGGAATTGTACAGATGACCCTGCGGCTCTGGGGACAGCAGCAGCCCATGGCTGTGTCCCCGTGCTCCCAGGCATGGCCTTGCCTTCCACCACACCGGAAGCCACTCAGGATGAGCCAGTGATGGACAGAGGCATGGGGGTTACATTGGAGGCTTTTACAGAAGCCAGTCTGAAGACTGGAGGACCCTGTCCAGAGCCCGTGGCCAGCAGAAGCAAGCTAAGAAAGCCCAAACCTGTCTCCCTGAGGAAGAAGATGGCCCCTGAACCGGAAATGTTGATGGAGGGTTCACCTCTCCCTAAGGCTTCCTCCCCATGGCTTCCTGATGGCTTGGATCAGAACGCGAATCCTAGTGTGCTGAGAGGCTCGGGGGCCCAGAGATCTCCCCTTAACCTTAAAGAAACAGCTGGTGTTCTCAGTAACGACACCAGTGACTCGGGGGTCGAGCTGCAGGGTGGGTCCAGGGACCCACCTCTCCAACTTGAGGATGATTTTACAGAAGATGGGGAGAATGTCAAGATCAGGAGCGCCCTTCCGAAGCAATCTGGCAGGAAACCCAGTAACAAACTGGCACCCAGTATACGAAAAGATGGCGTCAGTAAGCCAGTAGGTGTTGAGCAGCCTTCAGACCCAACAGTTCAAGATGCCTTGCTCGACCAGATGTCTCCTAAGCTAGATCCGAGTAAACGGAGTCATCCCCCCGCTAATTTCTTCGGGAGTGGCCCCATTCTGCAGAACTCCCCACCCCTCTCCTCCAAATGCTCCCACCACTTTGACCCAAATAACATTAACACTGATGACTCCGGGGATCCCTGCAAGCCAACCCCAGCTTTGACAAGCAGCGGCTTTTGTCCCGCCACTGGTAATCATGTTAATGAAATCTTAGACTCACCCAAGAAGGCAAAGTCACGTTTAATAACGAGCGGCTGCAAAGTGAAGAAATACGAAGCTCAGCCTCTCGATTTGGACGCGTGTTCCCAGGATGAAGGAGCCGTGATCTCGAAGATCTCAGAGATTCCCAACAGGGACGGCCACGCGACTGATGAGGAGAAGCTGGCCTCCACGTCGTCGTGTGCTCAGAAGTCAGCGGGGGCTGGGGTCAAA
Peptide sequence:
MALPSTTPEATQDEPVMDRGMGVTLEAFTEASLKTGGPCPEPVASRSKLRKPKPVSLRKKMAPEPEMLMEGSPLPKASSPWLPDGLDQNANPSVLRGSGAQRSPLNLKETAGVLSNDTSDSGVELQGGSRDPPLQLEDDFTEDGENVKIRSALPKQSGRKPSNKLAPSIRKDGVSKPVGVEQPSDPTVQDALLDQMSPKLDPSKRSHPPANFFGSGPILQNSPPLSSKCSHHFDPNNINTDDSGDPCKPTPALTSSGFCPATGNHVNEILDSPKKAKSRLITSGCKVKKYEAQPLDLDACSQDEGAVISKISEIPNRDGHATDEEKLASTSSCAQKSAGAGVKESREADEQLVAEVIEKCSPDTCSRSSENEAPQATVDSHPVKDVRGKAEHDVSKISVVRPFSIETRNCTDDPAALGTAAAHGCVPVLPGMALPSTTPEATQDEPVMDRGMGVTLEAFTEASLKTGGPCPEPVASRSKLRKPKPVSLRKKMAPEPEMLMEGSPLPKASSPWLPDGLDQNANPSVLRGSGAQRSPLNLKETAGVLSNDTSDSGVELQGGSRDPPLQLEDDFTEDGENVKIRSALPKQSGRKPSNKLAPSIRKDGVSKPVGVEQPSDPTVQDALLDQMSPKLDPSKRSHPPANFFGSGPILQNSPPLSSKCSHHFDPNNINTDDSGDPCKPTPALTSSGFCPATGNHVNEILDSPKKAKSRLITSGCKVKKYEAQPLDLDACSQDEGAVISKISEIPNRDGHATDEEKLASTSSCAQKSAGAGVK
IRESfinder result
IRESfinder score
0.306427906
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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