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mmu_circSuz12_696
Basic information of circular RNA
PanCircBase ID:
mmu_circSuz12_696
mm39_Chr. Position ID:
chr11|79901383|79913095|+
Gene name:
Suz12
Transcript:
ENSMUST00000163272.2
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
5
Exon/intron indexes:
5,6,7,8,9
Exon/intron offsets:
0,2806,4585,9121,11534
Genomic length:
11712
Exon/intron sizes in nt:
86,23,29,41,06,178
Flanking exon/introns:
chr11:79898388-79901383|chr11:79913095-79915735
Splice length:
696
Other database IDs:
CIRCpedia: MMU_CIRCpedia_214780, circAtlas: mmu-Suz12_0027
circBase: hsa_circ_0042975, circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_116444
Human Ortholog IDs:
Mature spliced Sequence:
ATAAGCCATCACAAAACTCAGAAAATGAACAAAATTCTGTTACCCTGGAAGTCCTGCTTGTGAAAGTTTGCCACAAAAAAAGGAAGGATGTAAGTTGTCCAATAAGACAAGTCCCTACTGGTAAAAAGCAGGTGCCTTTGAATCCTGACCTCAATCAAACAAAACCAGGAAATTTTCCATCCCTGGCAGTTTCCAGTAATGAATTTGAACCTAGTAACAGCCATATGGTGAAGTCCTACTCGTTGCTGTTTAGAGTAACTCGTCCAGGAAGAAGAGAATTTAATGGAATGATTAATGGAGAAACCAATGAAAATATTGATGTCAGTGAAGAACTTCCAGCCAGAAGAAAACGAAATCGCGAAGATGGAGAAAAGACATTTGTTGCACAAATGACAGTTTTTGACAAAAACAGGCGTTTACAGCTTTTAGATGGGGAATATGAAGTGGCCATGCAGGAAATGGAAGAATGTCCAATAAGTAAGAAAAGAGCAACATGGGAGACAATTCTTGATGGGAAGAGGCTGCCTCCATTTGAGACATTTTCTCAGGGACCTACATTACAATTTACTCTTCGATGGACAGGAGAAACCAACGATAAGTCTACAGCTCCTGTTGCCAAGCCTCTTGCCACTAGAAATTCAGAGAGCCTTCATCAGGAAAATAAGCCTGGTTCTGTTAAACCTGCACAAACTATTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
CTCCTGTTGCCAAGCCTCTT
60.25
TCACAAGCAGGACTTCCAGG
59.6
153
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GTCTACAGCTCCTGTTGCCA
59.68
TCACAAGCAGGACTTCCAGG
59.6
161
PCR template sequence:
AAGTCTACAGCTCCTGTTGCCAAGCCTCTTGCCACTAGAAATTCAGAGAGCCTTCATCAGGAAAATAAGCCTGGTTCTGTTAAACCTGCACAAACTATTGATAAGCCATCACAAAACTCAGAAAATGAACAAAATTCTGTTACCCTGGAAGTCCTGCTTGTGAAAGTTTGCCACAAAAAAAGGAAGGATGTAAGTTGTCC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
8
Interacting miRNAs (#binding sites)
mmu-miR-31-5p(1), mmu-miR-136-5p(1), mmu-miR-351-5p(1), mmu-miR-29b-1-5p(1), mmu-miR-182-5p(1), mmu-miR-29b-2-5p(1), mmu-miR-335-3p(1), mmu-miR-670-5p(1)
siRNAs for circular RNA silencing
Junction sequence
GCACAAACTATTGATAAGCCATCACA
siRNA sets
ATGGCTTATCAATAGTTTG, TGTGATGGCTTATCAATAG, GCTTATCAATAGTTTGTGC
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSUZ12_696_ORF_1
0.9698
0.046684872
0.994177399
1167
ORF sequence:
ATGGTGAAGTCCTACTCGTTGCTGTTTAGAGTAACTCGTCCAGGAAGAAGAGAATTTAATGGAATGATTAATGGAGAAACCAATGAAAATATTGATGTCAGTGAAGAACTTCCAGCCAGAAGAAAACGAAATCGCGAAGATGGAGAAAAGACATTTGTTGCACAAATGACAGTTTTTGACAAAAACAGGCGTTTACAGCTTTTAGATGGGGAATATGAAGTGGCCATGCAGGAAATGGAAGAATGTCCAATAAGTAAGAAAAGAGCAACATGGGAGACAATTCTTGATGGGAAGAGGCTGCCTCCATTTGAGACATTTTCTCAGGGACCTACATTACAATTTACTCTTCGATGGACAGGAGAAACCAACGATAAGTCTACAGCTCCTGTTGCCAAGCCTCTTGCCACTAGAAATTCAGAGAGCCTTCATCAGGAAAATAAGCCTGGTTCTGTTAAACCTGCACAAACTATTGATAAGCCATCACAAAACTCAGAAAATGAACAAAATTCTGTTACCCTGGAAGTCCTGCTTGTGAAAGTTTGCCACAAAAAAAGGAAGGATGTAAGTTGTCCAATAAGACAAGTCCCTACTGGTAAAAAGCAGGTGCCTTTGAATCCTGACCTCAATCAAACAAAACCAGGAAATTTTCCATCCCTGGCAGTTTCCAGTAATGAATTTGAACCTAGTAACAGCCATATGGTGAAGTCCTACTCGTTGCTGTTTAGAGTAACTCGTCCAGGAAGAAGAGAATTTAATGGAATGATTAATGGAGAAACCAATGAAAATATTGATGTCAGTGAAGAACTTCCAGCCAGAAGAAAACGAAATCGCGAAGATGGAGAAAAGACATTTGTTGCACAAATGACAGTTTTTGACAAAAACAGGCGTTTACAGCTTTTAGATGGGGAATATGAAGTGGCCATGCAGGAAATGGAAGAATGTCCAATAAGTAAGAAAAGAGCAACATGGGAGACAATTCTTGATGGGAAGAGGCTGCCTCCATTTGAGACATTTTCTCAGGGACCTACATTACAATTTACTCTTCGATGGACAGGAGAAACCAACGATAAGTCTACAGCTCCTGTTGCCAAGCCTCTTGCCACTAGAAATTCAGAGAGCCTTCATCAGGAAAATAAGCCTGGTTCTGTTAAACCTGCACAAACTATT
Peptide sequence:
MVKSYSLLFRVTRPGRREFNGMINGETNENIDVSEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPVAKPLATRNSESLHQENKPGSVKPAQTIDKPSQNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVSEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPVAKPLATRNSESLHQENKPGSVKPAQTI
IRESfinder result
IRESfinder score
0.598690076
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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