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mmu_circSupt3_982
Basic information of circular RNA
PanCircBase ID:
mmu_circSupt3_982
mm39_Chr. Position ID:
chr17|45234065|45359279|+
Gene name:
Supt3
Transcript:
ENSMUST00000050630.14
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
9
Exon/intron indexes:
3,4,5,6,7,8,9,10,11
Exon/intron offsets:
0,68555,71138,79911,110059,113533,114897,118037,125103
Genomic length:
125214
Exon/intron sizes in nt:
85,87,91,14,07,61,13,10,00,00,000
Flanking exon/introns:
chr17:45097597-45234065|chr17:45359279-45430029
Splice length:
982
Other database IDs:
circAtlas: mmu-Supt3_0016
Human Ortholog IDs:
Mature spliced Sequence:
GTTTTCTCTAGGTGATGCTAGGAGGCCTCTCCATGAAACTGCAGTTTTGGTGGAAGATGTGGTTCATACACAACTAATTAACCTGTTACAGCAAGCTGCAGAAGTCTCTCAGCTTCGGGGAGCAAGAGTGATCTCTGCTGAAGATCTTCTGTTCTTGATGAGAAAAGATAAGAAAAAACTTAGAAGACTTCTAAAGTACATGTTTATCCGAGACTACAAGTCAAAGATCATCAAAGGCATTGACGAGGATGACCTCCTGGAAGAAAAACTGAGTGGCAGCAGCGGTACAAACAAGAGACAGAAGATCGCTCAGGACTTGATCAACTCTATCGACCAGACAGGAGAGCTGCTGGCAATGTTTGAAGACAACGAGCTTGATGATGTCAAACAGGAGAGAATGGAGAGAGCAGAAAGACAGACTCGTACTATGGATTCAGCTCAATATGCAGAATTCTGTGAAAGCCGACAGTTAAGTTTCTCCAAAAAAGCTTCCAAATTCCGAGACTGGCTGGACTGCAGCAGCATGGAGATAAAACCCAATGTCATAGCTATGGAAATCCTGGCATATCTGGCATACGAAACCGTGGCCCAGCTGGTGGATCTGGCTCTTCTAGTGAGGCAAGATATGGTGTCCAAGGCAGGGGACCCCTTCAGCCATGCCATTTCTGCAACCTTCATTCAGTACCACAACTCTGCTGAGGGGTCCTCATGTTTGAAGTCCAGCCCAGACTCTCCTGAGAACACACCTCCTCCTACACCAACAGCTCCCTCTTCTGGATCACAGCATTCAGGTAGAGCTGCATCTGGTTCTTTGGGGAATGGGAACACAGGACAAGACACTGCCAAAACCAAGCAGCGGAAGAGGAAAAAGAGCACTGCGGCCTGTGGTGTCGAGGCTCACAGCGATGCCATCCAGCCCTGCCACATCAGAGAGGCCATTCGACGCTACGGCCACAAGATTGGCCCTCTCTCCCCATTCACA
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCCACATCAGAGAGGCCATT
60.11
TGCAGCTTGCTGTAACAGGT
60.18
162
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATCAGAGAGGCCATTCGACG
59.61
TGCAGCTTGCTGTAACAGGT
60.18
157
PCR template sequence:
CTGTGGTGTCGAGGCTCACAGCGATGCCATCCAGCCCTGCCACATCAGAGAGGCCATTCGACGCTACGGCCACAAGATTGGCCCTCTCTCCCCATTCACAGTTTTCTCTAGGTGATGCTAGGAGGCCTCTCCATGAAACTGCAGTTTTGGTGGAAGATGTGGTTCATACACAACTAATTAACCTGTTACAGCAAGCTGCA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
9
Interacting miRNAs (#binding sites)
mmu-miR-34c-5p(1), mmu-miR-34a-5p(1), mmu-miR-185-5p(1), mmu-miR-136-5p(1), mmu-miR-127-5p(1), mmu-miR-34b-5p(1), mmu-miR-5101(1), mmu-miR-1904(1), mmu-miR-296-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCCCCATTCACAGTTTTCTCTAGGT
siRNA sets
AGAGAAAACTGTGAATGGG, GAGAAAACTGTGAATGGGG, TAGAGAAAACTGTGAATGG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSUPT3_982_ORF_1
0.8938
0.253849421
0.996601157
1146
ORF sequence:
ATGGGAACACAGGACAAGACACTGCCAAAACCAAGCAGCGGAAGAGGAAAAAGAGCACTGCGGCCTGTGGTGTCGAGGCTCACAGCGATGCCATCCAGCCCTGCCACATCAGAGAGGCCATTCGACGCTACGGCCACAAGATTGGCCCTCTCTCCCCATTCACAGTTTTCTCTAGGTGATGCTAGGAGGCCTCTCCATGAAACTGCAGTTTTGGTGGAAGATGTGGTTCATACACAACTAATTAACCTGTTACAGCAAGCTGCAGAAGTCTCTCAGCTTCGGGGAGCAAGAGTGATCTCTGCTGAAGATCTTCTGTTCTTGATGAGAAAAGATAAGAAAAAACTTAGAAGACTTCTAAAGTACATGTTTATCCGAGACTACAAGTCAAAGATCATCAAAGGCATTGACGAGGATGACCTCCTGGAAGAAAAACTGAGTGGCAGCAGCGGTACAAACAAGAGACAGAAGATCGCTCAGGACTTGATCAACTCTATCGACCAGACAGGAGAGCTGCTGGCAATGTTTGAAGACAACGAGCTTGATGATGTCAAACAGGAGAGAATGGAGAGAGCAGAAAGACAGACTCGTACTATGGATTCAGCTCAATATGCAGAATTCTGTGAAAGCCGACAGTTAAGTTTCTCCAAAAAAGCTTCCAAATTCCGAGACTGGCTGGACTGCAGCAGCATGGAGATAAAACCCAATGTCATAGCTATGGAAATCCTGGCATATCTGGCATACGAAACCGTGGCCCAGCTGGTGGATCTGGCTCTTCTAGTGAGGCAAGATATGGTGTCCAAGGCAGGGGACCCCTTCAGCCATGCCATTTCTGCAACCTTCATTCAGTACCACAACTCTGCTGAGGGGTCCTCATGTTTGAAGTCCAGCCCAGACTCTCCTGAGAACACACCTCCTCCTACACCAACAGCTCCCTCTTCTGGATCACAGCATTCAGGTAGAGCTGCATCTGGTTCTTTGGGGAATGGGAACACAGGACAAGACACTGCCAAAACCAAGCAGCGGAAGAGGAAAAAGAGCACTGCGGCCTGTGGTGTCGAGGCTCACAGCGATGCCATCCAGCCCTGCCACATCAGAGAGGCCATTCGACGCTACGGCCACAAGATTGGCCCTCTCTCCCCATTCACA
Peptide sequence:
MGTQDKTLPKPSSGRGKRALRPVVSRLTAMPSSPATSERPFDATATRLALSPHSQFSLGDARRPLHETAVLVEDVVHTQLINLLQQAAEVSQLRGARVISAEDLLFLMRKDKKKLRRLLKYMFIRDYKSKIIKGIDEDDLLEEKLSGSSGTNKRQKIAQDLINSIDQTGELLAMFEDNELDDVKQERMERAERQTRTMDSAQYAEFCESRQLSFSKKASKFRDWLDCSSMEIKPNVIAMEILAYLAYETVAQLVDLALLVRQDMVSKAGDPFSHAISATFIQYHNSAEGSSCLKSSPDSPENTPPPTPTAPSSGSQHSGRAASGSLGNGNTGQDTAKTKQRKRKKSTAACGVEAHSDAIQPCHIREAIRRYGHKIGPLSPFT
IRESfinder result
IRESfinder score
0.502034246
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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