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mmu_circSupt16_96
Basic information of circular RNA
PanCircBase ID:
mmu_circSupt16_96
mm39_Chr. Position ID:
chr14|52414405|52414501|-
Gene name:
Supt16
Transcript:
ENSMUST00000046709.9
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
1
Exon/intron indexes:
12
Exon/intron offsets:
0
Genomic length:
96
Exon/intron sizes in nt:
96
Flanking exon/introns:
chr14:52414182-52414405|chr14:52414501-52414611
Splice length:
96
Other database IDs:
circBase: hsa_circ_0031166
Human Ortholog IDs:
Mature spliced Sequence:
AATGAGGATGATGAAGAGGAGGAGGAAGAGAAAGATGAGGCAGAGGACCTTTTGGGAAGAGGCTCTAGGGCGGCATTACTTACTGAAAGAACAAGG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCTAGGGCGGCATTACTT
59.89
TGCCTCATCTTTCTCTTCCTCC
59.5
77
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GCTCTAGGGCGGCATTACTT
59.89
TCCTCCTCCTCTTCATCATCCT
59.48
61
PCR template sequence:
CTTTTGGGAAGAGGCTCTAGGGCGGCATTACTTACTGAAAGAACAAGGAATGAGGATGATGAAGAGGAGGAGGAAGAGAAAGATGAGGCAGAGGAC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
NA
Interacting miRNAs (#binding sites)
NA
siRNAs for circular RNA silencing
Junction sequence
TGAAAGAACAAGGAATGAGGATGATG
siRNA sets
CATCATCCTCATTCCTTGT,ATCATCCTCATTCCTTGTT,CATCCTCATTCCTTGTTCT
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSUPT16_96_ORF_1
1.1965
0.011911342
0.166597548
189
ORF sequence:
ATGAGGATGATGAAGAGGAGGAGGAAGAGAAAGATGAGGCAGAGGACCTTTTGGGAAGAGGCTCTAGGGCGGCATTACTTACTGAAAGAACAAGGAATGAGGATGATGAAGAGGAGGAGGAAGAGAAAGATGAGGCAGAGGACCTTTTGGGAAGAGGCTCTAGGGCGGCATTACTTACTGAAAGAACAA
Peptide sequence:
MRMMKRRRKRKMRQRTFWEEALGRHYLLKEQGMRMMKRRRKRKMRQRTFWEEALGRHYLLKEQ
IRESfinder result
IRESfinder score
0.167605212
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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