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mmu_circStat3_425
Basic information of circular RNA
PanCircBase ID:
mmu_circStat3_425
mm39_Chr. Position ID:
chr11|100795732|100796754|-
Gene name:
Stat3
Transcript:
ENSMUST00000103114.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
8,7,6,5
Exon/intron offsets:
0,297,686,926
Genomic length:
1022
Exon/intron sizes in nt:
152,95,82,96
Flanking exon/introns:
chr11:100794588-100795732|chr11:100796754-100798816
Splice length:
425
Other database IDs:
circAtlas: mmu-Stat3_0003
circAtlas: hsa-SCAF8_0001, CIRCpedia: HSA_CIRCpedia_365332
Human Ortholog IDs:
Mature spliced Sequence:
CAAGGGGGCCAGGCCAACCACCCAACAGCCGCCGTAGTGACAGAGAAGCAGCAGATGTTGGAGCAGCATCTTCAGGATGTCCGGAAGCGAGTGCAGGATCTAGAACAGAAAATGAAGGTGGTGGAGAACCTCCAGGACGACTTTGATTTCAACTACAAAACCCTCAAGAGCCAAGGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGACCAGACAGAAGATGCAGCAGCTGGAACAGATGCTCACAGCCCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCTCTTGTCAGCAATGGAGTACGTGCAGAAGACACTGACTGATGAAGAGCTGGCTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATCGGAGGCCCTCCCAACATCTGCCTGGACCGTCTGGAAAACTG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GAGCTGGCTGACTGGAAGAG
60.11
ATGCTGCTCCAACATCTGCT
60.03
152
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
ATGAAGAGCTGGCTGACTGG
59.75
ATGCTGCTCCAACATCTGCT
60.03
157
PCR template sequence:
AGACACTGACTGATGAAGAGCTGGCTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATCGGAGGCCCTCCCAACATCTGCCTGGACCGTCTGGAAAACTGCAAGGGGGCCAGGCCAACCACCCAACAGCCGCCGTAGTGACAGAGAAGCAGCAGATGTTGGAGCAGCATCTTCAGGATGTCCGGAAGCGAGTGCAGGATC
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
3
Interacting miRNAs (#binding sites)
mmu-miR-676-3p(2), mmu-miR-207(1), mmu-miR-697(1)
siRNAs for circular RNA silencing
Junction sequence
GTCTGGAAAACTGCAAGGGGGCCAGG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSTAT3_425_ORF_1
1.2486
0.513446096
0.891144825
408
ORF sequence:
ATGTTGGAGCAGCATCTTCAGGATGTCCGGAAGCGAGTGCAGGATCTAGAACAGAAAATGAAGGTGGTGGAGAACCTCCAGGACGACTTTGATTTCAACTACAAAACCCTCAAGAGCCAAGGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGACCAGACAGAAGATGCAGCAGCTGGAACAGATGCTCACAGCCCTGGACCAGATGCGGAGAAGCATTGTGAGTGAGCTGGCGGGGCTCTTGTCAGCAATGGAGTACGTGCAGAAGACACTGACTGATGAAGAGCTGGCTGACTGGAAGAGGCGGCAGCAGATCGCGTGCATCGGAGGCCCTCCCAACATCTGCCTGGACCGTCTGGAAAACTGCAAGGGGGCCAGGCCAACCACCCAACAGCCGCCGTAG
Peptide sequence:
MLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENCKGARPTTQQPP
IRESfinder result
IRESfinder score
0.272830774
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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