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mmu_circSrrm2_225
Basic information of circular RNA
PanCircBase ID:
mmu_circSrrm2_225
mm39_Chr. Position ID:
chr17|24028942|24030055|+
Gene name:
Srrm2
Transcript:
ENSMUST00000190686.7
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
4
Exon/intron indexes:
5,6,7,8
Exon/intron offsets:
0,444,770,1062
Genomic length:
1113
Exon/intron sizes in nt:
78,63,33,51
Flanking exon/introns:
chr17:24027492-24028942|chr17:24030055-24030612
Splice length:
225
Other database IDs:
circAtlas: mmu-Srrm2_0008
Human Ortholog IDs:
Mature spliced Sequence:
CCTTGTTCGAGAGACCAGCAGTTCTCGCTCACCAACTCCAAAGCAAAAGAAGAAGAAAAAGAAGAAAGATAGAGGACGCAGGTCAGAGAGCAGTTCTCCTCGACGAGAGAGGAAGAAGAGTTCAAAGAAGAAGAAGCACAGGTCAGAATCTGAGTCCAAGAAACGGAAACACAGGTCTCCTACTCCAAAGAGCAAACGCAAATCAAAGGACAAGAAGAGGAAACG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
AACGGAAACACAGGTCTCCT
58.87
AGAACTGCTCTCTGACCTGC
59.39
161
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
AACGGAAACACAGGTCTCCT
58.87
TCTCTGACCTGCGTCCTCTA
59.38
153
PCR template sequence:
AGAAGAAGAAGCACAGGTCAGAATCTGAGTCCAAGAAACGGAAACACAGGTCTCCTACTCCAAAGAGCAAACGCAAATCAAAGGACAAGAAGAGGAAACGCCTTGTTCGAGAGACCAGCAGTTCTCGCTCACCAACTCCAAAGCAAAAGAAGAAGAAAAAGAAGAAAGATAGAGGACGCAGGTCAGAGAGCAGTTCTCCT
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
2
Interacting miRNAs (#binding sites)
mmu-miR-146a-5p(1), mmu-miR-146b-5p(1)
siRNAs for circular RNA silencing
Junction sequence
AGAAGAGGAAACGCCTTGTTCGAGAG
siRNA sets
NA
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
NA
NA
NA
NA
NA
ORF sequence:
NA
Peptide sequence:
NA
IRESfinder result
IRESfinder score
0.268956364
IRES/non IRES
non-IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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