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mmu_circSpg11_415
Basic information of circular RNA
PanCircBase ID:
mmu_circSpg11_415
mm39_Chr. Position ID:
chr2|121902535|121903486|-
Gene name:
Spg11
Transcript:
ENSMUST00000036450.8
CircRNA type:
Exonic circular (circ)RNA
Exon/Intron count:
2
Exon/intron indexes:
25,24
Exon/intron offsets:
0,791
Genomic length:
951
Exon/intron sizes in nt:
2,55,160
Flanking exon/introns:
chr2:121901546-121902535|chr2:121903486-121904984
Splice length:
415
Other database IDs:
CIRCpedia: MMU_CIRCpedia_5690, circAtlas: mmu-Spg11_0005
Human Ortholog IDs:
Mature spliced Sequence:
GTTGTCCAGTGAGTCCAGCAGCCAGTGGGCATTAGTGCTTCAGTTCTGCATGCTCCATGACAGGAAGCTGAGCGTGTCCTACCTTAGAGAATGTGCCAAAGCCAATGACTGGCTGCAGTTCCTGGTCCACAGCCAGCTGCATAACTACCACCCAGCAGAGGTGGAATCTCTTCTTCAGTATTTCAGTCCTGTCCTTCAGAGCCACTTGAAGCTGGCTTCTGAGAAACTGTCATCAGGGTCCATCTCTAGGGATGACAGCTGCCTCCAGGAACTCCAGAAAAACAAAGGAGAAACGAGTAATTTCTTTGAAATTCTCCACCGGTGCTCAGATGAGTCCACCTCTTGGAGCTGGCTCCTGGCTGAGGCAGTGAGACATCGGGCTCCCATCCTCAGTGTCCTGGCCTCGTGTGTGCAG
Divergent primers for circular RNA PCR
Primer Set-1
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGCTCAGATGAGTCCACC
60.11
CAGCTTCCTGTCATGGAGCA
60.04
165
Primer Set-2
Forward primer
Tm
Reverse primer
Tm
Product size
GGTGCTCAGATGAGTCCACC
60.11
CCTGTCATGGAGCATGCAGA
60.11
159
PCR template sequence:
CCACCGGTGCTCAGATGAGTCCACCTCTTGGAGCTGGCTCCTGGCTGAGGCAGTGAGACATCGGGCTCCCATCCTCAGTGTCCTGGCCTCGTGTGTGCAGGTTGTCCAGTGAGTCCAGCAGCCAGTGGGCATTAGTGCTTCAGTTCTGCATGCTCCATGACAGGAAGCTGAGCGTGTCCTACCTTAGAGAATGTGCCAAA
Note: Users can design additional primers for provided template sequence using Primer3 or NCBI primer-blast.
miRNAs associated with circular RNAs
No of miRNAs
1
Interacting miRNAs (#binding sites)
mmu-miR-324-3p(1)
siRNAs for circular RNA silencing
Junction sequence
CTCGTGTGTGCAGGTTGTCCAGTGAG
siRNA sets
GACAACCTGCACACACGAG
Protein-coding potential of circular RNAs
CPAT analysis
CPAT ORF ID
CPAT Fickett
CPAT Hexamer
Coding probabilty
ORF length
CIRCSPG11_415_ORF_1
0.9522
0.195408566
0.508734487
378
ORF sequence:
ATGCTCCATGACAGGAAGCTGAGCGTGTCCTACCTTAGAGAATGTGCCAAAGCCAATGACTGGCTGCAGTTCCTGGTCCACAGCCAGCTGCATAACTACCACCCAGCAGAGGTGGAATCTCTTCTTCAGTATTTCAGTCCTGTCCTTCAGAGCCACTTGAAGCTGGCTTCTGAGAAACTGTCATCAGGGTCCATCTCTAGGGATGACAGCTGCCTCCAGGAACTCCAGAAAAACAAAGGAGAAACGAGTAATTTCTTTGAAATTCTCCACCGGTGCTCAGATGAGTCCACCTCTTGGAGCTGGCTCCTGGCTGAGGCAGTGAGACATCGGGCTCCCATCCTCAGTGTCCTGGCCTCGTGTGTGCAGGTTGTCCAGTGA
Peptide sequence:
MLHDRKLSVSYLRECAKANDWLQFLVHSQLHNYHPAEVESLLQYFSPVLQSHLKLASEKLSSGSISRDDSCLQELQKNKGETSNFFEILHRCSDESTSWSWLLAEAVRHRAPILSVLASCVQVVQ
IRESfinder result
IRESfinder score
0.599224624
IRES/non IRES
IRES
riboCIRC information
riboCIRC ID
NA
NA
Evidence
Peptide sequence:
NA
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